25 results about "Penaeus monodon" patented technology

The invention discloses a breeding pressure simulation-based high-density-resistant penaeus monodon breeding method and system. The method comprises the following steps: determining pressure influence factors of penaeus monodon breeding through data retrieval, and extracting key environment variables by using principal component analysis; setting a plurality of groups of breeding pressure gradients, constructing a plurality of test groups by adopting an orthogonal test method, and collecting environment and phenotype data to evaluate the breeding effect and form a test data set. And constructing a breeding analysis model, and analyzing breeding evolution characteristics by constructing a correlation path and a topological structure chart of the breeding pressure gradient, the breeding effect and the monitoring data. And analyzing breeding demand information, inputting the breeding demand information into the model to obtain candidate breeding schemes, performing breeding feature extraction and cost evaluation on the candidate schemes, and finally recommending an optimal breeding scheme. The overall breeding benefit and production safety are improved, and the breeding period and complexity are reduced.

The invention relates to the technical field of prawn culture, in particular to a high-density culture optimization method for penaeus monodon based on multi-source culture environment analysis. A scale evaluation system of different preset cultivation environment condition combinations is built based on high-density penaeus monodon cultivation to evaluate the trend radiation diagram, whether the current cultivation density scale of the penaeus monodon is reasonable or not is analyzed through thermal polymerization after evaluation, and a multi-source cultivation environment analysis result is obtained; if one or more high-density abnormal breeding areas exist in a multi-source breeding environment analysis result, a simplex table model is constructed with the maximum bearing force as a constraint and the expected breeding density coefficient as a target function, and the optimal solution of the simplex table model is solved to determine the optimal breeding density scale of the high-density abnormal breeding areas. According to the method, the multi-source culture environment data can be fused to reasonably and scientifically analyze the high-density culture of the penaeus monodon and make an accurate and optimized culture strategy, so that the yield of the penaeus monodon per unit area is increased, and the low survival rate of the high-density culture is improved.

The invention relates to a penaeus monodon high temperature resistance character related SNP marker primer based on whole genome association analysis and application, and belongs to the field of molecular biology. The nucleotide sequence of the primer pair is shown in SEQ ID NO.1-2. An SNP molecular marker is located at the 27494780 site of the 27th chromosome of a penaeus monodon genome and is shown as Cgt; a mutation, and the A mutation is associated with a high temperature resistant character. The invention further provides a kit for detecting the high-temperature-resistant character of penaeus monodon, and high-temperature-resistant individuals can be screened by utilizing the primers and are used for breeding new varieties.

The invention discloses a method for increasing the survival rate of penaeus monodon in the storage and transportation process, and belongs to the field of alive-keeping transportation of aquatic products. The method comprises the steps of temporary rearing, gradient cooling dormancy treatment, oxygenation packaging and room-temperature storage. Wherein the cooling final temperature in the gradient cooling dormancy treatment step is 12 DEG C; a carrier used in oxygenation packaging is a foam box (the inner diameter is 390 mm * 270 mm * 200 mm; and the wall thickness is 20mm). By means of the method, physiological activities in the keep-alive process of the penaeus monodon can be effectively reduced, the stress response of the penaeus monodon can be reduced, the keep-alive survival rate of the penaeus monodon can be remarkably increased, meanwhile, equipment is simple, operation is easy, and certain economic benefits are achieved.

The invention discloses a penaeus monodon sperm ultralow-temperature preservation regulation and control method based on sperm motility detection, and relates to the technical field of aquatic product germplasm resources and genetic breeding, and the method comprises the following steps: collecting a penaeus monodon sperm sample, preprocessing the sample to carry out sperm motility detection, and utilizing deep learning to assist sperm motility analysis to obtain sperm motility data; collecting multiple omics data of the preprocessed sperm sample, and screening key molecular markers significantly related to sperm motility by using a knowledge graph to obtain omics characteristics; constructing a freezing injury prediction model according to the improved graph neural network, and inputting sperm motility data and omics characteristics to predict the freezing injury risk grade of the sperm sample; and dynamically adjusting a cryopreservation scheme based on the cryogenic injury risk grade of the sperm sample. According to the method, through a whole chain strategy of sperm motility detection, multi-omics analysis, artificial intelligence prediction and dynamic regulation and control, precise optimization of cryopreservation of the sperms of the penaeus monodon is realized.

The application discloses a strain of Sideropenaeus paramamosain bio-floc high-pond Shajia external sulfur red spirillum HDES1 and application of the strain in purifying inorganic nitrogen and phosphorus in aquaculture water, and belongs to the technical field of microorganisms. The Shajia external sulfur red spirillum HDES1 in the application is screened from a Sideropenaeus paramamosain bio-floc aquaculture high-pond, and has a preservation number of CCTCC NO: M 20191007. It is found through experiments that the strain has a remarkable purifying effect on high-concentration inorganic nitrogen and phosphorus in seawater aquaculture water or tail water, has no adverse effect on cultured Sideropenaeus paramamosain and seawater tilapia, and is helpful to improving the yield of fish and shrimp culture and realizing zero-water-change green ecological culture.

The application discloses a meteorological data-based penaeus monodon cultivation water body regulation method and system, and relates to the water quality regulation field.The method comprises the following steps: collecting penaeus monodon samples in different development states, determining corresponding development stages and corresponding water quality parameters, and generating a sample database; collecting the development state parameters of target penaeus monodon in a to-be-regulated cultivation tank, comparing the development state parameters with the sample database, determining the development stage and corresponding water quality requirements of the target penaeus monodon, obtaining cultivation water quality parameters under different meteorological conditions, and establishing a neural network model; inputting real-time meteorological data into the trained neural network model, outputting water quality parameter prediction values, judging whether the water quality meets the water quality requirements of the target penaeus monodon, and adaptively regulating the water quality that does not meet the requirements, so that the water quality parameters are maintained within a target range.The method effectively improves the accuracy and adaptability of penaeus monodon cultivation water quality management.

The invention discloses a penaeus monodon NFAT5 gene and application thereof, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the NFAT5 gene is as shown in SEQ ID NO: 2. The invention further discloses a recombinant expression vector containing the penaeus monodon NFAT5 gene, a host cell containing the recombinant expression vector, and an application of the NFAT5 gene. The invention further discloses application of the NFAT5 gene of the penaeus monodon, the protein, the recombinant expression vector containing the gene or the host cell containing the expression vector to preparation of a gene preparation or a feed additive for improving the low-salt tolerance of the penaeus monodon.

The invention relates to a decomposition flora performance evaluation method and system for penaeus monodon culture, and belongs to the technical field of penaeus monodon culture. Organic waste decomposed by decomposition flora is subjected to a simulation test, a plurality of groups of three-dimensional models of organic waste changes are obtained, the maximum degradation rate is calculated according to the three-dimensional models of the organic waste changes, and the degradation rate is calculated according to the maximum degradation rate. And constructing a performance evaluation model to evaluate and judge the optimal organic waste degradation rate to obtain a final decomposition flora performance evaluation result, obtaining the quantity of the currently put decomposition flora, and calculating by combining the final decomposition flora performance evaluation result to obtain the degradation rate of the organic waste. The actual degradation amount of the current decomposition flora on the organic waste is obtained, the difference between the actual degradation amount and the predicted amount generated by the organic waste is judged, and a penaeus monodon breeding optimization scheme is generated. According to the method, the performance of the decomposition flora for penaeus monodon culture can be efficiently and accurately evaluated, and the culture quality and efficiency are improved.

The invention discloses a penaeus monodon DNA (deoxyribonucleic acid) fingerprint spectrum which comprises at least 209 specific SNP (single nucleotide polymorphism) sites and realizes efficient and accurate distinguishing of different culture populations, so that technical guarantee is provided for identification and traceability work of penaeus monodon germplasm, and meanwhile, an accurate molecular technology platform for germplasm resource development, utilization and protection is built. The invention further discloses a construction method of the DNA fingerprint spectrum of the penaeus monodon and application of the DNA fingerprint spectrum of the penaeus monodon in identification of germplasm resources of the penaeus monodon and assistant breeding.

The present application relates to a method and system for breeding of Marsupenaeus japonicus based on selection of uniformity of growth traits, belonging to the technical field of genetic breeding of Marsupenaeus japonicus. A plurality of breeding base parents of Marsupenaeus japonicus are obtained, a plurality of the breeding base parents are identified and evaluated by genotyping technology, and a plurality of the genomic combination information is introduced into a blank pairing table to obtain a uniformity pairing table of growth traits of Marsupenaeus japonicus. The coefficient of variation of the simulated growth record information is calculated based on the pairing table, the abnormality determination result of the growth traits is generated, the correlation degree of the operating mechanism and the environmental data is calculated based on the abnormality determination result of the growth traits to generate a correlation degree ranking table, the regulation scheme of the breeding equipment is formulated according to the correlation degree ranking table, the influence degree of the seedlings after regulation is calculated by establishing a linear regression model, and the final breeding scheme is obtained. The present application can solve the problem of genetic gene mutation caused by environmental differences in the breeding process of Marsupenaeus japonicus and improve the uniformity of growth traits.

This invention discloses a Penaeus monodon NFAT5 gene and its application. The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the amino acid sequence of the protein encoded by the NFAT5 gene is shown in SEQ ID NO:2. This invention also discloses a recombinant expression vector containing the Penaeus monodon NFAT5 gene, a host cell containing the recombinant expression vector, and the use of the Penaeus monodon NFAT5 gene, protein, recombinant expression vector containing the gene, or host cell containing the expression vector in the preparation of gene preparations or feed additives for improving the low salt tolerance of Penaeus monodon.

The invention relates to a method for cultivating a new variety of high-temperature-resistant fast-growing penaeus monodon, which belongs to the field of aquatic breeding and comprises the following steps of: performing extreme high-temperature acute stress for 4-7 days at a juvenile penaeus monodon stage of a batch constructed family and performing high-temperature long-term cultivation stress for 45-60 days at a juvenile penaeus monodon stage; performing weighted comprehensive evaluation on growth and survival rates of different families under a high-temperature condition by combining growth parameters including average survival time of individuals of the extreme high-temperature acute stress families and average survival rates and average weights of the high-temperature long-term bred families; and screening out family individuals which grow quickly and have strong high-temperature tolerance, carrying out breed conservation and cultivation to obtain parent parents, and carrying out passage and generation breeding to obtain the new high-temperature-resistant fast-growing penaeus monodon variety.

This invention relates to a method and system for hatching shrimp larvae based on multimodal data fusion, belonging to the field of tiger prawn hatching technology. The invention constructs a reinforcement learning framework based on digital twins and twin model mechanisms, ultimately acquiring image data information of the current target aquaculture area within a preset time period. The reinforcement learning framework is then used to analyze this image data information to find the action sequence that maximizes cumulative rewards. This invention systematically solves the technical challenges of extensive perception, lagging regulation, and experience-dependent decision-making during shrimp larvae hatching through the deep integration of multimodal perception, digital twin modeling, and reinforcement learning decision-making. It significantly improves the survival rate and growth uniformity of larvae during the hatching stage, while reducing the risk of water quality deterioration and energy and material consumption, providing reliable technical support for intelligent aquaculture management.

The present application relates to a kind of Penaeus monodon intelligent transport device and method, belong to Penaeus monodon transport technical field, including transport car and transport box, the box inner layer is provided with communication module and first sensor, the communication module is used to obtain the geographical position of current transport car, to obtain the weather information corresponding to the geographical position of current transport car by big data network, the first sensor is used to obtain the temperature value of current box inner layer, to adjust the temperature of the box inner layer according to the weather information and temperature value, and the box inner layer is divided into multiple placement areas, and adjusting mechanism is provided in the placement area. It can be automatically adjusted according to the actual weather information of the transport environment of Penaeus monodon, so that it is not adjusted under the survival environment of Penaeus monodon, so that the transport process is more in line with intelligence, so that the survival environment of Penaeus monodon is more in line with the survival environment of Penaeus monodon in the transport process.

The invention belongs to the field of multi-nutrition-level polyculture ponds, and particularly relates to a trachinotus ovatus-penaeus monodon-blue crab multi-nutrition-level polyculture pond which comprises a pond body, a lower-layer separation net is slidably connected to the lower middle portion of the inner wall of the pond body, and an upper-layer separation net is slidably connected to the upper middle portion of the inner wall of the pond body. The lower end of the upper-layer separation net is fixedly connected with a plurality of evenly-distributed supporting legs, and the upper end of the upper-layer separation net is fixedly connected with a supporting rod. The upper-layer separation net, the lower-layer separation net, the dismounting and mounting mechanism and other structures are additionally arranged in the pond body, in the using process, the pond body can be divided into three layers for breeding through the upper-layer separation net and the lower-layer separation net, independent species can be separated, mutual predation among bred objects can be effectively avoided, and the breeding efficiency is improved. In addition, in the breeding process, the bottom of the pond body can be oxygenated through an air conveying pipe, a rotary oxygenation frame and other structures at the bottom, and oxygenation of the water body in the pond body can be more uniform.

This patent discloses a cryopreservation solution and method for improving the sperm survival rate of Penaeus monodon, comprising a base solution and combined cryoprotectants. The base solution is a Penaeus monodon sperm cell culture medium containing Penaeus monodon serum; the combined cryoprotectants include permeable and non-permeable cryoprotectants. By using Penaeus monodon sperm cell culture medium instead of traditional calcium-free saline as the base solution for cryopreservation, an optimized environmental basis is established for cryopreservation, significantly improving cryoprotection efficiency. The cryopreservation method provided in this application enables Penaeus monodon sperm to achieve a survival rate of over 89% after 10 months of cryopreservation, achieving long-term stable preservation of sperm under ultra-low temperature conditions; its key biological activity indicators show no significant difference compared to before cryopreservation, successfully achieving artificial insemination. This provides key technical support for the long-term preservation of Penaeus monodon germplasm resources and lays the foundation for the construction of germplasm banks.

This invention relates to the field of aquaculture technology, specifically a feed formulation and preparation method for improving the reproductive performance of broodstock tiger prawns. The method includes: determining the specific nutritional requirements of broodstock tiger prawns during gonadal development; screening core animal and plant protein sources and determining their basic mass ratios accordingly; performing targeted biological treatment on the core raw materials to enrich gonadotropin-active small molecules; compounding these with phospholipids, attractants, vitamins, and minerals to form a functional additive combination; mixing the core raw materials and additive combination in proportion, granulating, and adding antioxidants during processing; and finally verifying the formulation effect and confirming the final compound feed through standardized aquaculture trials. The compound feed obtained based on this method can effectively replace traditional live feed, improve broodstock tiger spawning, hatching rate, and larval quality, and has advantages such as balanced nutrition, stable quality, and suitability for large-scale production, solving the industry problems of live feed being prone to pathogens, supply fluctuations, and high costs.

This invention provides a method for improving the stress resistance of Penaeus monodon in aquaculture, comprising the following steps: ① disinfection and water filling of the culture pond; ② water quality adjustment and cultivation; ③ introduction of anti-stress supplementary pellets; ④ feed cultivation; ⑤ release of shrimp larvae; ⑥ feeding management; and ⑦ post-cultivation management. This invention improves the stress resistance of Penaeus monodon by introducing anti-stress supplementary pellets and adding anti-stress components to the feed, thereby achieving a shrimp larvae survival rate of 40.9%, a body length of 30.6 mm, and a weight of 1.35 g, resulting in higher aquaculture yields and greater economic benefits for farmers.

The invention discloses a penaeus monodon cultivation management method and system suitable for industrial aquaculture, and belongs to the field of aquaculture intelligent management, and the method specifically comprises the steps: collecting environment parameters and penaeus monodon activity data in real time through a water quality sensor and a camera unit in a plurality of penaeus monodon cultivation cycles; performing regional clustering and grouping based on regional environment parameter change analysis and breeding density statistics to form a plurality of regional clusters; performing multivariate linear fitting and long-short period feature analysis on the breeding environment parameters and density of the regional cluster to generate an environment-density association record table, and constructing a decision tree prediction model according to the association table; real-time environment data is imported into the model to predict the breeding density, prawn activity migration prediction is carried out, and then the breeding environment is regulated and controlled. According to the method, the problems of single environment monitoring dimension and lack of multi-parameter coupling and culture density prediction analysis in traditional prawn culture are solved, the problem of decision hysteresis is solved, and the culture efficiency is effectively improved.

The invention discloses a cryopreservation solution for improving the survival rate of sperms of penaeus monodon and a cryopreservation method thereof.The cryopreservation solution comprises a basic solution and a combined protective agent, and the basic solution is a penaeus monodon sperm cell culture medium and contains penaeus monodon serum; the combined protective agent comprises a permeable protective agent and a non-permeable protective agent. A prawn sperm cell culture medium is used for replacing traditional calcium-free normal saline to serve as a basic solution of the cryopreservation solution, an optimized environment basis is established for cryopreservation, and the cryopreservation efficiency is remarkably improved. According to the cryopreservation method provided by the invention, the survival rate of the freeze-thawed sperms of the penaeus monodon cryopreserved for 10 months can be greater than 89%, and long-term stable preservation of the sperms under an ultralow temperature condition can be realized; the key biological activity index is not obviously different from that before cryopreservation, and artificial insemination is successfully realized. The method provides a key technical support for long-term preservation of Penaeus monodon germplasm resources, and lays a foundation for construction of a germplasm bank.

The invention provides enterococcus faecium R8 and application thereof, and relates to the technical field of microorganisms, the enterococcus faecium R8 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.15229, and the preservation date is January 17, 2018. The enterococcus faecium R8 is named as enterococcus faecium R8. The invention further provides a preparation method of the enterococcus faecium R8. According to the method, the optimal effective concentration of enterococcus faecium is defined, and the conversion and utilization rate of prawns to the feed can be effectively increased, so that the growth of the prawns is accelerated while the culture cost is saved. Meanwhile, the multi-enhancement effect of the enterococcus faecium on the immune system of the penaeus monodon is effectively improved, the immune defense system of the penaeus monodon is activated and enhanced, and the anti-oxidative stress capability of the penaeus monodon is enhanced, so that the resistance of the penaeus monodon to diseases is possibly improved.

The application discloses umami peptides derived from Penaeus monodon and application thereof, and belongs to the technical field of bioactive peptides. The umami peptides are 11 in number, and the amino acid sequences are respectively ACWVPCEK, QRMMQ, DAKKACW, DRM, RMM, PDPDPT and the like, as shown in SEQ ID NO. 1-11. The umami peptides are applied in umami agents. The umami peptides are obtained from the myoglobin of Penaeus monodon by using a rapid screening technology, wherein the umami intensity of QRMMQ is the strongest, the umami threshold of PDPDPT is the lowest, and ACWVPCEK has a synergistic umami effect. The application enriches the umami peptide library of marine origin, improves the economic value of Penaeus monodon, and simultaneously studies key amino acids and an action mechanism of umami peptide binding with umami receptors, thereby providing a reference for studying the umami mechanism of umami peptides.

The present invention relates to a feed composition suitable for feeding crustaceans comprising mannose oligosaccharides. The invention also relates to the use of mannose oligosaccharide compositions for feeding crustaceans. The invention also relates to crustaceans as a food source, in particular shrimp of the species Litopenaeus vannamei or Penaeus monodon, fed with the feed composition comprising mannose oligosaccharides.

The present invention belongs to the field of aquaculture, and specifically relates to a method for intensive seedling rearing of large-sized Penaeus monodon in chloride-type saline-alkali water medium in mid-latitude regions. The method includes steps such as the design and construction of intensive seedling rearing ponds, preparations before fry stocking, fry stocking, intensive rearing management, feeding management, bottom substrate management, growth detection, disease prevention and control, thinning and grading of fry, etc. The present invention makes full use of chloride-type saline-alkali ponds in mid-latitude regions. By building a multi-arch greenhouse, the cost is reduced and the firmness is enhanced; various nutrient salts in the soil are used to cultivate beneficial bacteria to decompose the organic matter generated by residual baits and feces, improving the self-purification ability of the pond; biological baits are inoculated and cultivated before fry stocking, reducing the bait cost and improving the immunity of the prawns; attachment substrates are set up to increase the attachment area of juvenile prawns, reducing the probability of fry cannibalism and increasing the survival rate of intensive seedling rearing. The present invention has low breeding cost, effectively increases economic benefits, and is of great significance for increasing export earnings and solving labor employment, etc.