153 results about "Penaeus monodon" patented technology

The invention relates to a fingerling desalination method for the freshwater culture of a tiger prawn, in particular to the culturing of the seawater economic prawn in freshwater, belonging to the aquaculture technical field. The invention is characterized in that the method comprises the following technique steps: preparing a temporary prawn larva breeding pond; cleaning up the temporary prawn larva breeding pond; sterilizing the temporary prawn larva breeding pond; fertilizing; artificial oxygen increasing facility; the pond affusion and salinity regulating; temporary prawn larva breeding; prawn larva stocking and breeding; prawn larva desalting and salinity regulating; prawn larva cultivating; cleaning up a main breeding pond; communicating the main breeding pond and the temporary prawn larva breeding pond; the freshwater breeding of the prawn larva. The invention can prevent the prawn larva from dying resulted from the sudden change of an osmotic pressure after directly entering the freshwater through the desalting process during the temporary breeding period of the prawn larva, leading the tiger prawn to be able to grow from the seawater into the freshwater after artificial desalting and breeding, which is beneficial to reduce the incidence of the tiger prawn, improve the surviving rate of stocking and breeding of the prawn larva and increase the culturing yield.

The invention discloses a wild-imitation breeding method for river eel. The method comprises the following steps: A, preparing for pond conditions and other work; B, stocking seeds, wherein the seeds include river eel, penaeus monodon, chub and bighead; C, feedstuff feeding; D, daily management: (1) aerating by adopting an aerator; (2) disinfecting a water body; (3) applying a microbial preparation; (4) changing water; (5) controlling diseases: preventing, controlling and treating diseases by using Chinese Herbal medicines; E, modifying an artificial wild-imitation environment. According to the method, river eel, penaeus monodon, chub and bighead are bred in a breeding pond in a mixed way, so that the stability of a breeding system can be improved; meanwhile, the water quality environment of the pond can be improved, and the breeding benefit of unit area is increased; a marine drug prepared by compounding natural Chinese herbal medicines has the advantages of natural property, multi-functionality, small toxic and side effects and no drug resistance, the quality of marine products is improved remarkably, and the immunologic functions of aquatic animals are improved.

The invention discloses a penaeus monodon larva stage microparticle feed comprising protein source raw materials, fat source raw materials, sugar source raw materials, antioxidants, phagostimulant, multi vitamins and complex mineral salts. The feed can improve survival rate of penaeus monodon at a larva stage, enables the larvae to be produced fast and stable under water, and rapid in ingestion, has the advantages of being convenient to use and low in the pollution of the environment and the like, and solves the problems that current organism baits are unstable in quality and quantity, prawn slices on the market are unbalanced in nutrition, water contamination is too serious to control at a larva breeding stage and the like; accordingly, healthy development of penaeus monodon larva production and breeding is promoted, and the feed is of great significance.

The invention provides a penaeus monodon industrialized high-density cultivating technology, comprising a young shrimp coarse standard pond, an intermediate cultivating pond, an adult shrimp cultivating pond, a rotary-drum microstrainer, an air-sifter type protein separator, a positive-negative biological processor, a purifying device, a sedimentation tank, an algae biological processor and a controller. According to the penaeus monodon industrialized high-density cultivating technology, the problems that the prawn survival rate is low, the benefit and yield are not prominent, the occupied area is overlarge, and the environmental pollution is severe are fundamentally solved through cultivating penaeus monodon by utilization of industrialized circulating water; by employment of grading multistage cultivation, the aquaculture water space can be sufficiently utilized, the water quality is guaranteed, the possibility of disease occurrence is reduced, the cultivating success rate is improved, drugs are prevented from being used, and the food safety is guaranteed; organic carbon is added in the aquaculture water through a biological floccules device so as to adjust the proportion of carbon to nitrogen, and sufficient oxygenation is combined at the same time, so that the growth of heterotrophic bacteria in the water can be promoted, ammonia nitrogen and nitrite nitrogen are reduced up to 98-100%, the feed quantity required for cultivating creatures is reduced, and the benefit is increased.

This invention discloses an ecological polyculturing method of sea cucumber, Penaeus monodon and Portunus trituberculatus, which comprises the following steps: choosing the Portunus trituberculatus and Penaeus monodon with quick growth speed and high economic value based on the biological characteristics that the sea cucumber and decapod crustacean have different growth time and nutrition levels;and making full use of the idle water and abundant natural baits in the pond during the aestivation of the sea cucumbers to polyculture sea cucumbers, shrimps and crabs. The culturing method is characterized by optimizing the ecological environment of the pond, reducing pollution discharge and disease spread, using the water space effectively, reducing culturing and producing cost, increasing culturing output, etc.

The present invention relates to a composition useful as prophylactic and / or therapeutic agent for the management of viral and bacterial diseases in aquatic animals, said composition containing effective amount of extract obtained from the plants Lantena camera, Aegle marmelos, Ocimum sanctum, Mimosa pudica, Cynodon dactylon, Curcuma longa, and Allium sativum, optionally in combination with a pharmaceutically acceptable carrier, diluents or excipients.

The invention discloses a penaeus monodon breeding method. The method includes the steps of firstly, selecting a breeding pool, wiping the inner wall of the breeding pool through kaempferia galanga slices and then exposing the breeding pool after exposing the breeding pool, coating the inner wall of the breeding pool with liquid medicine and then exposing the breeding pool, repeating the coating and exposing steps, mixing quicklime with water, spraying the mixture into the breeding pool, and then exposing the breeding pool; secondly, controlling the pool water temperature, the pH value and the salinity, throwing shrimp seeds into the breeding pool, throwing shrimp seed fodders two to three times a day from the first day to the twentieth day, and throwing adult shrimp fodders seven to eight times a day twenty days later; thirdly, injecting oxygen into pool water of the breeding pool every two to three days, replacing water of the breeding pool every four to five days, and throwing virustat into the breeding pool every thirty to forty days. By means of the method, the autoimmunity and resistance of penaeus monodon can be enhanced, growth speed of the penaeus monodon is increased, and the survival rate and meat quality are increased.

The invention discloses compound feed special for penaeus monodon breeding season nutrient enrichment and a preparation method thereof. The compound feed comprises Peru fish powder, sleeve-fish meat powder, shrimp meat powder, pork liver powder and the like, can replace conventional fresh baits, solves the problems that source and quality of the fresh baits are unstable, also can promote gonad development of parental shrimps, and improves egg laying amount, egg hatchability and larva abnormal rate of penaeus monodon parental shrimps; spread of viral diseases among penaeus monodon is reduced, production of healthy penaeus monodon larvae is ensured, and stability of the compound feed under water is good, the compound feed is easy for the parental shrimps to absorb, water environment pollution in aquiculture areas is reduced, and therefore the compound feed has popularization and application value.

The invention discloses microsatellite marker primers used for penaeus monodon microsatellite family identification. The microsatellite marker primers include six primer pairs in total, and the six primer pairs are PM-38, PM-69, PM-92, PM-84, PM-102 and PM-114 respectively. The invention further discloses an identification method for the penaeus monodon microsatellite family and application of the microsatellite marker primers to penaeus monodon microsatellite family identification. By the adoption of the microsatellite marker primers, a paternity test platform is set up on penaeus monodon with polymorphic microsatellite markers marked by fluorescence for the first time, and the identification accuracy reaches 99%; different families and sources of the penaeus monodon can be effectively and fast identified, and a basis is provided for breeding, reproduction matching and enhancement and releasing assessment of the penaeus monodon.

The invention discloses a combined method of a stereo ecological polyculture mode and the prevention and control of hepatopancreatic necrosis disease and white spot syndrome. Specifically, 3 days before adding prawn seeds, 200 scatophagus argus fries with length of 3-5cm and 300 siganids fries with length of 2-3cm are added into every Mu of a breeding pond; when prawn seeds are to be added, a prawn seed addition amount is 80000-100000 seeds / Mu; when prawn length reaches 4-5cm, 5-6 Nile perch with length of 8-10cm and 10-15 hybrid giant tiger groupers with length of 8-10cm are added into every Mu of the pond. According to the invention, fishes with different feeding habits are cultured with prawns at a same time, such that diseases and weak prawns are eliminated in time, prawn disease infection source is eliminated, and a water cleaning function is realized. A polyculture mode of prawns and fishes with suitable specifications is started from a breeding early-mid stage, such that a prawn disease infection source is cut off, and hepatopancreatic necrosis disease and white spot syndrome outbreak is prevented. The breeding mode is optimized, such that prawn breeding success rate is improved. The method provided by the invention is suitable for the breeding of prawns such as litopenaeus vannamei, marsupenaeus japonicas, penaeus monodon and the like.

This invention relates to the methods of detecting Penaeus monodon baculovirus (MBV). Two methods are established: the first one is a polymerase chain reaction (PCR) and the second one is an ELISA. For the PCR method, two sets of primers are designed. The first set of primers is designed from the conserved sequences of nuclear polyhedrosis viruses (NPVs) DNA polymerase genes. The second set of primers is designed from the genomic DNA of MBV. The antibody for ELISA is an antiserum against the occlusion bodies of MBV.

The invention discloses a layered fish and shrimp circulating water polyculture system. The system is composed of a culture pond I (1), a culture pond II (3), a culture pond III (5), a culture pond IV (6), a settling pond I (8), a purifying pond I (10), a clear water pond I (11), a settling pond II (17), a purifying pond II (26) and a clear water pond II (23), wherein the culture pond I (1), the culture pond II (3), the culture pond III (5), the settling pond II (17), the purifying pond II (26) and the clear water pond II (23) are connected in series to form a penaeus monodon culture system, and the culture pond IV (6), the settling pond I (8), the purifying pond I (10) and the clear water pond I (11) are connected in series to form a tilapia mossambica culture system. The system mainly has the advantages that high-density culture of penaeus monodon is achieved; water purifying capacity is high, and drainage rate is low; synchronous culture of penaeus monodon and carnivorous fishes is achieved; separate control of fish and shrimp circulating water can be conducted easily.

The invention relates to preparation and application methods of penaeus monodon fermented feed. The preparation method comprises the following steps: respectively carrying out enlarged culture on bacillus licheniformis, lactobacillus plantarum and candida utilis, then mixing in a volume ratio of 1:1:1, adding brown sugar (2g / 1000mL) and running water into the mixed bacterial solution under the condition that the ratio of the culture solution to water is 1:80, uniformly mixing, uniformly stirring with penaeus monodon compound feed in a ratio of 2:5, sealing with a plastic film, and fermenting for 18-28 hours, so that the fermented feed is obtained. The invention also provides the application method of the fermented feed. The prepared fermented feed is used for feeding penaeus monodon once a day, and the feeding amount is 20-30% of the daily total feeding amount. The prepared fermented feed can obviously improve nitric oxide synthase activity, superoxide dismutase activity, catalase activity, phenoloxidase activity, glutathione peroxidase activity and total antioxidant capacity of penaeus monodon and obviously reduce the malondialdehyde content; and composition of florae in intestines of the penaeus monodon is obviously improved, and the survival rate of penaeus monodon culture is obviously increased.

The invention discloses a shell solidifying mixed feed for penaeus monodon and a preparation method thereof. Raw materials of the feed consist of the following components in percentage by weight: 15-30 percent of fish meal, 5-10 percent of meat and bone meal, 1-5 percent of chiken meat meal, 1-5 percent of housefly larvae powder, 10-20 percent of fermented soybean meal, 10-15 percent of peanut bran, 1-5 percent of beer yeast, 1-5 percent of cuttlefish cream, 1-5 percent of shrimp head meal, 15-25 percent of strong flour, 1-5 percent of sea fish oil, 1-3 percent of soybean lecithin, 0.5-1.5 percent of calcium dihydrogen phosphate, 0.1-0.5 percent of calcium amino acid chelate, 0.1-0.5 percent of cholesterol, 0.01-0.1 percent of bacillus subtilis, 0.1-0.5 percent of spirulina powder, 0.1-0.5 percent of vitamin C-phosphate ester (35 percent), 0.05-0.1 percent of vitamin D3, 0.1-0.5 percent of composite multivitamins and 0.1-0.5 percent of composite mineral salt. The shell solidifying mixed feed for the penaeus monodon effectively meets the complete demand of the shrimps on nutritive materials at special stages of castoff and renew; the situations such as soft shell or failure of castoff of the shrimps are obviously improved; and the immunity of the penaeus monodon is obviously improved.

The invention discloses a jellyfish breeding method including subjecting jellyfishes to mixed culture with ruditapes philippinarum, sinonovacula constrictas, penaeus chinensis, penaeus monodons and goby; controlling ecological breeding technologies including a stocking system based on breeding species and density, a feeding system based on a match between the species and number and a water quality control system based on a principle that firstly applying fertilizer into water, secondly maintaining fertilizer content and thirdly controlling the water quality. In this way, the jellyfishes and other species are rapid to grow and high in output and economic benefit. By the use of the jellyfish breeding method, the jellyfish output reaches 200-400kg / mu, the outputs of the ruditapes philippinarum and the sinonovacula constrictas reach 200-300kg / mu, and the outputs of the penaeus chinensis and the penaeus monodons reach 10-30kg / mu, and the goby output reaches 20-30kg / mu.

The invention relates to the technical field of aquaculture and specifically relates to a penaeus monodon feed. The feed is prepared from the following raw materials in parts by weight: 25-30 parts of maize meal, 12-15 parts of fried bean powder, 10-12 parts of rapeseed cake, 4-5 parts of pumpkin seeds, 6-8 parts of wheat middling, 4-5 parts of watermelon peel, 4-5 parts of viviparous powder, 2-3 parts of fish meal, 4-5 parts of snail powder, 2-3 parts of medical stone powder, 5-6 parts of sudangrass, 2-3 parts of shrimp chaff, 4-5 parts of fermented soya beans, 0.2-0.5 parts of radix bupleuri, 0.2-0.5 parts of astragalus membranaceus, 0.2-0.5 parts of salviae miltiorrhizae, 0.2-0.5 parts of golden cypress, 0.5-1 part of donkey-hide gelatin, a proper amount of salt, and 4-5 parts of phagostimulant. The feed prepared in the invention is rich in material type, satisfies the omnivorous characteristics of the penaeus monodon, and is rich in various fine protein materials; active ingredients in the traditional Chinese medicine components such as radix bupleuri, astragalus membranaceus, salviae miltiorrhizae, golden cypress and donkey-hide gelatin are combined, so that the growth and development of the penaeus monodon can be effectively promoted, weight gain can be quickened up and culture benefits can be improved; moreover, all materials of the feed are pure natural green materials, and a drug residue problem is avoided.

The invention discloses a method for carrying out integrated aquaculture of ictalurus punctatus in a pond industrial aquaculture system. The method is scientific and feasible in principle, easy to operate and implement in production, and capable of being adopted and applied by farmers easily in economic benefit. The method comprises the steps of preparation before planting and aquaculture, fry stocking, purification zone floating bed rice planting, planting and aquaculture management, aquatic species capturing and rice harvesting. Accordingly, by means of the proper stocking density of ictalurus punctatus fingerlings in an aquaculture water tank, the stocking size is improved, and by means of the annual aquaculture, commercial longsnout catfish with the best-selling size being about 1.5 kg / fish are sold at a high price at different times; by properly stocking a proportion of silver carp, bighead carp, myxocyprinus asiaticus, penaeus monodon and snails and planting 5-8% of floating bedrice in an ecological purification zone simultaneously, and by means of the integrated planting and aquaculture measure, the in-situ remediation effect on aquaculture water is better exerted, the ecological benefit, comprehensive output and economical benefit of the whole breeding system are significantly increased, and the method has strong practical application and popularization value.

The invention discloses a compound feed capable of quickly recovering body color of blue-body penaeus monodon. The compound feed comprises the following components in parts by weight of 30-45 parts offish meal, 3-6 parts of chicken meat powder, 17-20 parts of soybean meal, 3-10 parts of peanut cakes, 17-20 parts of low-gluten flour, 1-3 parts of shrimp paste, 1-3 parts of squid paste, 2-5 parts of soybean phospholipid oil, 5-10 parts of crayfish powder, 5-10 parts of euphausia superba powder, 0.2-0.4 part of xanthophyll, 1-2 parts of bentonite and 1-2 parts of a mixture of multiple vitamins and multiple minerals. The compound feed further comprises the following components of 0.2-0.5 part of choline chloride, 0.2-0.5 part of sodium chloride, 1-2 parts of calcium bis(dihydrogenphosphate),0.01-0.03 part of an antioxidant and 0.05-0.1 part of a feed mildewproof agent. The compound feed disclosed by the invention has good color mixing effects, can replace a compound feed with astaxanthin, can quickly recover the body color of the blue-body penaeus monodon, is low in cost and can effectively increase breeding benefits. The invention further provides a preparation method of the compound feed. The preparation method is simple, and large-scale production is convenient to realize.

The invention provides a compound enzyme feed additive capable of improving growth rate and non-specific immunity of prawns and application of the compound enzyme feed additive. The additive comprises mannase, xylanase, cellulase, a saccharifying enzyme, lipase, alpha-amylase, beta-amylase and neutral protease. The components are compounded and proportioned with a special carrier and are added into prawn feed by an additive amount of 0.2-0.3%. The product is environmentally friendly, can be used for industrialized production on a large scale, and a novel growth feed additive is provided for aquatic feed. The compound enzyme feed additive provided by the invention can remarkably improve growth of penaeus monodon, has the purposes of improving true nutritive value and reducing the food coefficient, and has promoting effects to different extents to activities of intestinal amylase, lipase of hepatopancreas and serum SOD (Superoxide Dismutase). Compared with additives with similar functions, the additive provided by the invention has the advantages of safety, specifity, no residues, no side effects and the like.

The invention discloses a traditional Chinese herbal medicinal feed additive for preventing balantidiasis of penaeus monodon. The body immunity of penaeus monodon can be effectively reinforced when 0.5-1 percent of the additive is added into the feed for penaeus monodon, and occurrence of balantidiasis in the process of breeding penaeus monodon can be effectively prevented, and the additive does not have adverse influence to the palatability of feed, and does not have pollution, residue or side effect when being used in penaeus monodon breeding, and is safe and environment-friendly. The additive has the advantages that 1, a mode that extracting Chinese herbal medicines and then adding is adopted, and the digestive tract of penaeus monodon is short, so that the additive can play a better effect in preventing balantidiasis; 2, the functional components in the traditional Chinese herbal medicines are extracted by a 'ultrasonic-assisted two-step extraction method', so that the function of the traditional Chinese herbal medicinal feed additive for preventing balantidiasis of penaeus monodon is further improved; 3, the raw materials in the additive are wide in source and low in prices, and the production processing process is simple.

The invention discloses a high-efficient culture method for prawns. The method comprises the following steps: before putting young prawns, sufficiently sunning a pond and carrying out disinfection, then filtering water sources, fertilizing water, and diluting fertilized water when the young prawns are placed; when the young prawns are put, pouring powerful effective microorganisms (EM) and beneficial bacteria prawn protecting drugs, wherein the use amounts of EM and the beneficial bacteria prawn protecting drugs are 1 kilogram per mu (a unit of area equal to 0.0667 hectares); while managing young prawns, feeding powdered high nutrient feeds three times a day at regular hours and in fixed quantities, and observing materials on a material platform and lengths of the prawns; during culture of the prawns, within the first month after the prawns are thrown, changing water every five days, wherein 5-10 cm deep water is changed each time, within the first to third months after the prawns are thrown, changing water every seven days, wherein 10-20 cm deep water is changed each time, and starting a waterwheel aerator everyday, wherein the waterwheel aerator operates for 4 to 5 hours in rainy days and 2 to 3 hours in sunny days. Compared with the prior art, the prawns are fed by the powdered high nutrient feeds, thereby being capable of effectively improving the growth and enhancing the immune function of Penaeus monodon to shorten the growth periods of the prawns so as to improve the culture efficiency.

The invention discloses an indoor multilayer three-dimensional circulating aquaculture system for penaeus monodon. The system is characterized in that a culture pond, a water circulating treatment pond, a biological purification pond and a water storage pond communicate to form a circulating water path; the water circulating treatment pond is provided with a water treatment mechanism for purifying water, the biological purification pond is provided with a biological filtration mechanism for further purifying water, and the water storage pond is provided with a water body adjusting mechanism for adjusting water body parameters; and plurality of layers of culture planes formed by bolting silk mesh cloth are arranged in the culture pond, a plurality of culture planes which are arranged at intervals are contained in the same layer, and the front ends of the culture planes incline downwards, so that a plurality of culture water layers are formed in the culture pond. The system has the characteristics of being flexible, capable of fully utilizing culture water, low in operation cost, energy-saving and environmentally friendly, and can effectively increase the habitat and culture area of the penaeus monodon and increase the culture yield. The invention further discloses an aquaculture method using the aquaculture system.

The invention discloses a technique for sustainable aquaculture of litopenaeus vannamei and penaeus monodon in ecological ponds. The area of each shrimp pond is not fixed and is generally 20 mu, in regard to each pond, the pond bottom is sandy mud or silty, the water quality is better, the pollution is less, planktons are rich, the proportion of seawater is about 1.024, the pH value is about 8.0,each shrimp pond has the water depth of 2 meters and is provided with a water inlet and a water outlet which are independent, and the water inlets and the water outlets are preferably separated independently, so that the irrigation and drainage requirements of shrimp aquaculture are met, and automatic irrigation and drainage cannot be carried out in a neap season. According to the technique for sustainable aquaculture of litopenaeus vannamei and penaeus monodon in the ecological ponds, solar exposure for pond bottoms and the comprehensive pond disinfection enable germs and bacteria in the shrimp ponds are eradicated, and harm during aquaculture of litopenaeus vannamei and penaeus monodon is avoided; regular water quality detection enables data in a water body convenient to control, accordingly the prevention effect is achieved, diseases during shrimp aquaculture are minimized, litopenaeus vannamei aquaculture in summer is avoided, and the disease frequency during aquaculture is minimized.

The invention discloses three-dimensional cultivation technology for a seawater shoal pond, which is characterized by comprising the following steps: drying the pond after shrimps are produced in winter, and removing 10 to 20 centimeters of silt on the bottom surface of the pond; feeding water to soak the pond in the middle of February, and meanwhile performing biological agent sterilization; feeding water into the pond until the depth of water is 1.2 to 1.5 meters in the middle of March; fertilizing 1 kilogram of diammonium phosphate to water in each mu, propagating photosynthetic bacteria and seaweed to form biological baits, and controlling the salinity to between 2 to 3 percent and the pH value to between 7 and 8.6; putting seedlings of sinonovacula constricta in the middle of February, seedlings of penaeus chinesis or Japanese penaeus monodon between April 20 and May 10 and seedlings of swimming crabs in the middle of May; and enriching the water once in the middle of each month in a mode of feeding fresh small conches serving as the main baits and fish and shrimps serving as the auxiliary baits, and performing little water exchange every ten days. The technology solves the problems of single variety, low yield and poor benefit of the current seawater shoal pond cultivation, and is suitable for three-dimensional cultivation for the sinonovacula constricta, the swimming crabs, the penaeus chinesis or the Japanese penaeus monodon in the seawater shoal pond.

The invention belongs to the field of prawn farming and breeding, and discloses a method for the semi-closed ecological desalination cultivation of penaeus monodon. The method comprises the steps: building an ecological cultivation cement pit in a semi-closed ecological cultivation workshop, and then carrying out the ecological desalination cultivation of penaeus monodon. The method comprises the specific steps: building the ecological cultivation workshop, building an ecological desalination cultivation cement pit, clearing the bottom of the cement pit, carrying out water planting, placing seedlings, carrying out desalination management and water quality regulation and control, carrying out feeding management, and collecting young shrimps. Through the building of the semi-closed cultivation cement pit, the method achieves the desalination cultivation of the penaeus monodon, and creates a natural growth environment for the young penaeus monodon. After the desalination cultivation, the young penaeus monodon is healthy and strong and is high in adaptive capacity. The size of the young penaeus monodon can be greater than 1.5cm, and the rate of survival is improved by 25%, thereby facilitating the reduction of the cost in big water ecological cultivation of the penaeus monodon, and improving the cultivation benefits.

The invention provides a special compound feed for breeding parent prawns of penaeus monodon. The feed comprises the following components in percentage by weight: 25%-40% of fish meal, 5%-9.5% of artemia powder, 3%-6% of enzymolysis fish soluble pulp powder, 3%-9% of schizochytrium limacinum powder, 1%-3% of kelp powder, 2%-8% of hermetia illucens powder, 2%-5% of shrimp shell powder, 6%-12% of mussel powder, 2%-5% of marine red yeast dry powder, 10%-15% of flour, 1.5%-3% of lysolecithin, 0.8%-1.4% of ARA purified oil, 1.5% of DHA purified oil, 1.5% of EPA purified oil, 3.1%-3.7% of stearic acid triglyceride, 1%-2% of fish oil, 1%-5% of monocalcium phosphate, 0.9%-1.22% of vitamin premix and 1.08%-1.4% of mineral premix. According to the special compound feed, the growth performance of the parent prawns of penaeus monodon in the breeding period is remarkably improved, gonad development of the penaeus monodon is promoted, and the egg laying amount, the egg laying rate, the continuous egg laying rate, the fertilized egg hatching rate, the metamorphosis rate and the egg diameter of the parent prawns are increased.

The invention provides a gene diagnosis reagent box and its detecting method for aquatic animal (like cyprinoid, Penaeus monodon, Epinephelus awoara, ormer, etc) and human pathogens-Vibrio fluvialis, designed by using a pair of primers designed by the sequence in conservative region of Vibrio fluvialis gene to act as main body. It adopts polyase chain reaction (PCR) technique to qualitatively detect the specific DNA fragments of the Vibrio fluvialis, simply and conveniently, and rapidly, good-specificity, and high-sensitivity; can be used in bacteria tracking detection of aquatic animal pathogens in the course of breeding in each period, and also the clinic detection of human intestinal acute infections, as well as environmental monitoring, avoiding the pathogens infecting and popularizing and it has very great practical value.

The invention discloses a low-salt-resistant penaeus monodon new species breeding method. Low-salt-resistant penaeus monodon species can be better sorted through breeding in a salinity regulation modewithin multiple stepped ranges. According to the low-salt-resistant penaeus monodon new species breeding method, a stepped range mode, rather than a mode in which a certain low salinity value maintains invariable fundamentally, is adopted, in this way, the process in which the salinity of a breeding water body changes to a low salinity direction naturally can be simulated better, and thus the sorted penaeus monodon species has higher capability to adapt to the situation that the salinity is influenced because river water rises suddenly or rainstorm is heavy in a natural state.

The invention discloses a penaeus monodon test device. The penaeus monodon test device comprises a wall, a plurality of monitoring assemblies embedded in the wall and a test assembly arranged at one side of the wall, wherein the test assembly comprises a plurality of test tubes which are horizontally arranged; the heads and tails of adjacent test tubes communicate through tee joints; the pluralityof test tubes are sequentially arranged in a layered manner along a vertical direction; the plurality of test tubes can simulate sea areas with different depths; a simulation groove is arranged at the bottom part of each test tube; a seabed material is arranged in each simulation groove; the test assembly comprises supports and a plurality of probes distributed in the length direction of each support at intervals; the probes can move in the length direction of the supports; and the test assembly can monitor penaeus monodon.

The invention discloses a breeding method for promoting turtle growth. The breeding method comprises the following steps of S1, preparation before breeding, wherein a pond of 4-6 mu is selected as a breeding pond, and potamogeton crispus and waterweed are planted at the bottom of the breeding pond after disinfection treatment; S2, disinfection putting, wherein penaeus monodon, fish fry, escargots and little turtle are put into the breeding pond after disinfection; S3, feed putting and daily management, wherein feed is fed twice at a fixed point every day, and fishing is conducted after feeding is conducted for 28-30 months; in the breeding process, the water body change is observed every day, water is replaced timely, oxygenation is conducted for 2-4 hours every day in summer, and disease prevention is conducted once every half a month. Through the method of combining stocking and artificial breeding, the water body resource is made full use of, the turtle is excellent in meat quality, good in condition and fast in weight increasement, the come-out time of turtles is shortened, fishes and shrimps can be harvested simultaneously, and the overall economic efficiency of turtle breeding is increased.