13 results about "Penaeus monodon" patented technology

The application discloses a strain of Sideropenaeus paramamosain bio-floc high-pond Shajia external sulfur red spirillum HDES1 and application of the strain in purifying inorganic nitrogen and phosphorus in aquaculture water, and belongs to the technical field of microorganisms. The Shajia external sulfur red spirillum HDES1 in the application is screened from a Sideropenaeus paramamosain bio-floc aquaculture high-pond, and has a preservation number of CCTCC NO: M 20191007. It is found through experiments that the strain has a remarkable purifying effect on high-concentration inorganic nitrogen and phosphorus in seawater aquaculture water or tail water, has no adverse effect on cultured Sideropenaeus paramamosain and seawater tilapia, and is helpful to improving the yield of fish and shrimp culture and realizing zero-water-change green ecological culture.

The invention relates to a decomposition flora performance evaluation method and system for penaeus monodon culture, and belongs to the technical field of penaeus monodon culture. Organic waste decomposed by decomposition flora is subjected to a simulation test, a plurality of groups of three-dimensional models of organic waste changes are obtained, the maximum degradation rate is calculated according to the three-dimensional models of the organic waste changes, and the degradation rate is calculated according to the maximum degradation rate. And constructing a performance evaluation model to evaluate and judge the optimal organic waste degradation rate to obtain a final decomposition flora performance evaluation result, obtaining the quantity of the currently put decomposition flora, and calculating by combining the final decomposition flora performance evaluation result to obtain the degradation rate of the organic waste. The actual degradation amount of the current decomposition flora on the organic waste is obtained, the difference between the actual degradation amount and the predicted amount generated by the organic waste is judged, and a penaeus monodon breeding optimization scheme is generated. According to the method, the performance of the decomposition flora for penaeus monodon culture can be efficiently and accurately evaluated, and the culture quality and efficiency are improved.

The invention discloses a penaeus monodon DNA (deoxyribonucleic acid) fingerprint spectrum which comprises at least 209 specific SNP (single nucleotide polymorphism) sites and realizes efficient and accurate distinguishing of different culture populations, so that technical guarantee is provided for identification and traceability work of penaeus monodon germplasm, and meanwhile, an accurate molecular technology platform for germplasm resource development, utilization and protection is built. The invention further discloses a construction method of the DNA fingerprint spectrum of the penaeus monodon and application of the DNA fingerprint spectrum of the penaeus monodon in identification of germplasm resources of the penaeus monodon and assistant breeding.

The present application relates to a method and system for breeding of Marsupenaeus japonicus based on selection of uniformity of growth traits, belonging to the technical field of genetic breeding of Marsupenaeus japonicus. A plurality of breeding base parents of Marsupenaeus japonicus are obtained, a plurality of the breeding base parents are identified and evaluated by genotyping technology, and a plurality of the genomic combination information is introduced into a blank pairing table to obtain a uniformity pairing table of growth traits of Marsupenaeus japonicus. The coefficient of variation of the simulated growth record information is calculated based on the pairing table, the abnormality determination result of the growth traits is generated, the correlation degree of the operating mechanism and the environmental data is calculated based on the abnormality determination result of the growth traits to generate a correlation degree ranking table, the regulation scheme of the breeding equipment is formulated according to the correlation degree ranking table, the influence degree of the seedlings after regulation is calculated by establishing a linear regression model, and the final breeding scheme is obtained. The present application can solve the problem of genetic gene mutation caused by environmental differences in the breeding process of Marsupenaeus japonicus and improve the uniformity of growth traits.

This invention discloses a Penaeus monodon NFAT5 gene and its application. The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the amino acid sequence of the protein encoded by the NFAT5 gene is shown in SEQ ID NO:2. This invention also discloses a recombinant expression vector containing the Penaeus monodon NFAT5 gene, a host cell containing the recombinant expression vector, and the use of the Penaeus monodon NFAT5 gene, protein, recombinant expression vector containing the gene, or host cell containing the expression vector in the preparation of gene preparations or feed additives for improving the low salt tolerance of Penaeus monodon.

This invention relates to a method and system for hatching shrimp larvae based on multimodal data fusion, belonging to the field of tiger prawn hatching technology. The invention constructs a reinforcement learning framework based on digital twins and twin model mechanisms, ultimately acquiring image data information of the current target aquaculture area within a preset time period. The reinforcement learning framework is then used to analyze this image data information to find the action sequence that maximizes cumulative rewards. This invention systematically solves the technical challenges of extensive perception, lagging regulation, and experience-dependent decision-making during shrimp larvae hatching through the deep integration of multimodal perception, digital twin modeling, and reinforcement learning decision-making. It significantly improves the survival rate and growth uniformity of larvae during the hatching stage, while reducing the risk of water quality deterioration and energy and material consumption, providing reliable technical support for intelligent aquaculture management.

The invention belongs to the field of multi-nutrition-level polyculture ponds, and particularly relates to a trachinotus ovatus-penaeus monodon-blue crab multi-nutrition-level polyculture pond which comprises a pond body, a lower-layer separation net is slidably connected to the lower middle portion of the inner wall of the pond body, and an upper-layer separation net is slidably connected to the upper middle portion of the inner wall of the pond body. The lower end of the upper-layer separation net is fixedly connected with a plurality of evenly-distributed supporting legs, and the upper end of the upper-layer separation net is fixedly connected with a supporting rod. The upper-layer separation net, the lower-layer separation net, the dismounting and mounting mechanism and other structures are additionally arranged in the pond body, in the using process, the pond body can be divided into three layers for breeding through the upper-layer separation net and the lower-layer separation net, independent species can be separated, mutual predation among bred objects can be effectively avoided, and the breeding efficiency is improved. In addition, in the breeding process, the bottom of the pond body can be oxygenated through an air conveying pipe, a rotary oxygenation frame and other structures at the bottom, and oxygenation of the water body in the pond body can be more uniform.

This patent discloses a cryopreservation solution and method for improving the sperm survival rate of Penaeus monodon, comprising a base solution and combined cryoprotectants. The base solution is a Penaeus monodon sperm cell culture medium containing Penaeus monodon serum; the combined cryoprotectants include permeable and non-permeable cryoprotectants. By using Penaeus monodon sperm cell culture medium instead of traditional calcium-free saline as the base solution for cryopreservation, an optimized environmental basis is established for cryopreservation, significantly improving cryoprotection efficiency. The cryopreservation method provided in this application enables Penaeus monodon sperm to achieve a survival rate of over 89% after 10 months of cryopreservation, achieving long-term stable preservation of sperm under ultra-low temperature conditions; its key biological activity indicators show no significant difference compared to before cryopreservation, successfully achieving artificial insemination. This provides key technical support for the long-term preservation of Penaeus monodon germplasm resources and lays the foundation for the construction of germplasm banks.

This invention relates to the field of aquaculture technology, specifically a feed formulation and preparation method for improving the reproductive performance of broodstock tiger prawns. The method includes: determining the specific nutritional requirements of broodstock tiger prawns during gonadal development; screening core animal and plant protein sources and determining their basic mass ratios accordingly; performing targeted biological treatment on the core raw materials to enrich gonadotropin-active small molecules; compounding these with phospholipids, attractants, vitamins, and minerals to form a functional additive combination; mixing the core raw materials and additive combination in proportion, granulating, and adding antioxidants during processing; and finally verifying the formulation effect and confirming the final compound feed through standardized aquaculture trials. The compound feed obtained based on this method can effectively replace traditional live feed, improve broodstock tiger spawning, hatching rate, and larval quality, and has advantages such as balanced nutrition, stable quality, and suitability for large-scale production, solving the industry problems of live feed being prone to pathogens, supply fluctuations, and high costs.

This invention provides a method for improving the stress resistance of Penaeus monodon in aquaculture, comprising the following steps: ① disinfection and water filling of the culture pond; ② water quality adjustment and cultivation; ③ introduction of anti-stress supplementary pellets; ④ feed cultivation; ⑤ release of shrimp larvae; ⑥ feeding management; and ⑦ post-cultivation management. This invention improves the stress resistance of Penaeus monodon by introducing anti-stress supplementary pellets and adding anti-stress components to the feed, thereby achieving a shrimp larvae survival rate of 40.9%, a body length of 30.6 mm, and a weight of 1.35 g, resulting in higher aquaculture yields and greater economic benefits for farmers.

The invention provides enterococcus faecium R8 and application thereof, and relates to the technical field of microorganisms, the enterococcus faecium R8 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.15229, and the preservation date is January 17, 2018. The enterococcus faecium R8 is named as enterococcus faecium R8. The invention further provides a preparation method of the enterococcus faecium R8. According to the method, the optimal effective concentration of enterococcus faecium is defined, and the conversion and utilization rate of prawns to the feed can be effectively increased, so that the growth of the prawns is accelerated while the culture cost is saved. Meanwhile, the multi-enhancement effect of the enterococcus faecium on the immune system of the penaeus monodon is effectively improved, the immune defense system of the penaeus monodon is activated and enhanced, and the anti-oxidative stress capability of the penaeus monodon is enhanced, so that the resistance of the penaeus monodon to diseases is possibly improved.

The application discloses umami peptides derived from Penaeus monodon and application thereof, and belongs to the technical field of bioactive peptides. The umami peptides are 11 in number, and the amino acid sequences are respectively ACWVPCEK, QRMMQ, DAKKACW, DRM, RMM, PDPDPT and the like, as shown in SEQ ID NO. 1-11. The umami peptides are applied in umami agents. The umami peptides are obtained from the myoglobin of Penaeus monodon by using a rapid screening technology, wherein the umami intensity of QRMMQ is the strongest, the umami threshold of PDPDPT is the lowest, and ACWVPCEK has a synergistic umami effect. The application enriches the umami peptide library of marine origin, improves the economic value of Penaeus monodon, and simultaneously studies key amino acids and an action mechanism of umami peptide binding with umami receptors, thereby providing a reference for studying the umami mechanism of umami peptides.

The present invention relates to a feed composition suitable for feeding crustaceans comprising mannose oligosaccharides. The invention also relates to the use of mannose oligosaccharide compositions for feeding crustaceans. The invention also relates to crustaceans as a food source, in particular shrimp of the species Litopenaeus vannamei or Penaeus monodon, fed with the feed composition comprising mannose oligosaccharides.