The invention discloses an ant colony optimization processing method of large-scale multi-target intelligent moving route selection; after data of NTSP target logistics delivery addresses, distances between every two addresses, and M price of cost for passing through each route are obtained, a route planning unit is solved by ant colony optimization technology so as to obtain a specific walking route for intelligent mobile-agent delivery, and the route is outputted to an executive mechanism for realization. When the method is used to solve the problem of large-scale multi-target intelligent moving route selection, the invention has good optimization performance, and has the advantages of parallelism, self-organization, strong robustness, and the like, and the obtained solutions are large in quantity, high in quality, and have strong approximation capability to the real Pareto solution set; the obtained solution set has uniform distribution; the calculation speed is high. The inventioncan be used in intelligent processing units of route planning systems in fields of logistics distribution, intelligent traffic, internet, robots, etc.