Application of cantharidin for preventing and treating osteosarcoma as miR-214-3p/Wnt/beta-Catenin signal pathway inhibitor

A signaling pathway and osteosarcoma technology, applied in the field of biomedicine, can solve the problems of complex pathogenic factors, lack of clinically effective treatment drugs, high degree of malignancy of osteosarcoma, and achieve the effect of solving high mortality

Pending Publication Date: 2020-07-14
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0004] Due to the high degree of malignancy of osteosarcoma, complex pathogenic factors, unclear pathogenesis, lack of clinically effective therapeutic drugs, a

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  • Application of cantharidin for preventing and treating osteosarcoma as miR-214-3p/Wnt/beta-Catenin signal pathway inhibitor
  • Application of cantharidin for preventing and treating osteosarcoma as miR-214-3p/Wnt/beta-Catenin signal pathway inhibitor
  • Application of cantharidin for preventing and treating osteosarcoma as miR-214-3p/Wnt/beta-Catenin signal pathway inhibitor

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[0037] The present invention will be further elaborated below in conjunction with the accompanying drawings and specific embodiments. These examples should be understood as only for illustrating the present invention but not for limiting the protection scope of the present invention. After reading the contents of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent changes and modifications also fall within the scope defined by the claims of the present invention.

[0038] (1) xCELLigence real-time cell detector detects proliferation ability

[0039] Using E-Plate plate, add 100 μl of serum-containing complete medium containing different concentrations of cantharidin (1.25 μM, 2.5 μM, 5 μM, 7.5 μM, 10 μM, 15 μM) to each well, and DMSO (1 / 10000 V / V) as a blank control, 0.5 μM doxorubicin is used as positive control; set up the program, measure the background; prepare cells, inoculate U2OS and 1...

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Abstract

The invention discloses an application of cantharidin in preparation of a drug for preventing and treating osteosarcoma growth and metastases as an miR-214-3p/Wnt/beta-Catenin signal pathway inhibitor. The invention provides a novel method which can be used for preventing and treating osteosarcoma occurrence, development, metastases and drug resistance. The problems of a high death rate and shortsurvival time of a patient with osteosarcoma can be effectively solved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the use of cantharidin as an inhibitor of miR-214-3p / Wnt / β-Catenin signaling pathway in the prevention and treatment of growth and metastasis of osteosarcoma. Background technique [0002] Osteosarcoma (OS) is the most common primary malignant bone tumor. There are two peaks of incidence, which are children / adolescents aged 10-14 and the elderly over 65 years old. Children / adolescents frequently occur . The main clinical symptoms of osteosarcoma are pain, mass, and limb movement impairment. The clinical and pathological changes are bone destruction, which is characterized by osteolysis. The course of the disease is characterized by high malignancy, high heterogeneity, rapid growth, and early metastasis. . [0003] Osteosarcoma is often misdiagnosed and the diagnosis and treatment of the disease are delayed because the pain is not obvious in the early stage and the changes ...

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Application Information

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IPC IPC(8): A61K31/365A61P35/00A61P35/04
CPCA61K31/365A61P35/00A61P35/04
Inventor 杨燕萍胡少朴王拥军常君丽阮红峰王雯艺支文兰王晓波梁倩倩刘书芬
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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