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Apparatus and method for clinical or non-clinical acquired immuno-deficiency syndrome virus detection by using saliva

A technology of HIV and saliva, which is applied in the field of laboratory medicine, can solve the problems of insufficient total immunoglobulin content and the inability to detect specific antibodies, and achieve the effects of safe and easy collection, fast detection, and simple and convenient use

Inactive Publication Date: 2009-02-18
GUANGZHOU YIXI DRINK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Third, the content of total immunoglobulin in oral secretions may not be high enough to detect specific antibodies

Method used

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  • Apparatus and method for clinical or non-clinical acquired immuno-deficiency syndrome virus detection by using saliva
  • Apparatus and method for clinical or non-clinical acquired immuno-deficiency syndrome virus detection by using saliva
  • Apparatus and method for clinical or non-clinical acquired immuno-deficiency syndrome virus detection by using saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: The device for clinical or non-clinical detection of HIV using saliva according to the present invention

[0054] The saliva detection kit for AIDS virus diagnosis of the present invention, such as figure 1 shown, including:

[0055] A strip-shaped detection box 1, one end is designed as a hand-held end 11, and the other end is provided with a round hole 12, and a ring-shaped fixing seat 13 protrudes upwards from the round hole, and the surface of the box body between the two ends is opened. A long and narrow window 14; the box body of the detection box 1 is sequentially equipped with an anti-overflow adsorption layer, a sponge medium, a cellulose adsorption layer and a nitrocellulose membrane (not shown in the figure); the bottom of the round hole 12 A rectangular window is provided on the nitrocellulose membrane, on which a positive control substance and a recombinant type I and type II HIV virus peptide mixture are immobilized;

[0056] Cylindrical li...

Embodiment 2

[0066] Embodiment 2: The detection method of the device for clinical or non-clinical detection of HIV using saliva according to the present invention

[0067] Place the device and collected saliva samples at room temperature 20-30 minutes before starting the procedure.

[0068] The inspection process, once started, must continue to the end, any interruption may lead to undesired results.

[0069] Such as figure 2 As shown, install the liquid collection filter 2 on the detection box 1, specifically align the bottom end of the liquid collection filter 2 with the protruding ring-shaped fixing seat on the detection box 1, and then plug the bottom end of the liquid collection filter 2 Into the annular fixed seat, because the size of the bottom of the liquid collection filter 2 is consistent with the size of the annular fixed seat on the detection box 1, the liquid collection filter 2 can be stably fixed on the detection box 1.

[0070] Such as image 3 As shown, buffer reagent ...

Embodiment 3

[0083] Embodiment three: Utilize saliva according to the present invention to carry out the sensitivity and specificity determination of the device of clinical or non-clinical detection HIV

[0084]The saliva detection kit and detection method for AIDS virus diagnosis in the present invention are obtained by detecting 437 samples. These saliva samples and corresponding serum samples were collected from research institutes or hospitals and clinics in the Petit Cameroon area of ​​Paris, France, where immigrants from India, Thailand and West Africa live. All serum samples were tested by the following three FDA-approved HIV diagnostic kits: Abbott's ELISA kit, Wellcozyme's ELISA kit, and Cambridge Biotech's western blot kit . Each corresponding saliva sample is detected by the saliva detection kit and detection method for HIV diagnosis of the present invention.

[0085] The test results are shown in Table 1, Table 2 and Table 3:

[0086] Table 1

[0087] Serum enzy...

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Abstract

The invention belongs to the laboratory medicine technology field, which relates to a device and a method of utilizing saliva to execute clinical or non-clinical detecting HIV / AIDS virus. The device of utilizing saliva to execute clinical or non-clinical detecting HIV / AIDS virus includes a strip shape test kit, a columned liquid collecting filter, a piston, a sampling cup, a measuring pipette, phosphate buffer reagent and antihuman IgG antibody-gold colloid conjugate reagent. The invention also provides a method of using the device to execute clinical or non-clinical detecting for saliva. Through detecting the saliva sample, the invention can achieve the requirements of accurate, quick, convenient and secure clinical or non-clinical detecting of HIV / AIDS virus.

Description

technical field [0001] The invention belongs to the technical field of laboratory medicine, and relates to a saliva detection kit and a detection method, in particular to a device and a method for clinically or non-clinically detecting HIV using saliva. Background technique [0002] AIDS is a sexually transmitted disease with no cure and a high fatality rate. With the rapid spread of AIDS around the world in recent years, the infection rate of the disease in China is on the rise year by year. If calculated according to the annual growth rate of about 25%-30%, the total number of HIV carriers in China will exceed 10 million by 2010. If effective measures are not taken immediately, China will become one of the countries with the largest number of HIV infections in the world. Therefore, China has reached a critical moment in preventing and controlling the spread of AIDS. [0003] Accurate, fast, simple and safe HIV testing methods are the prerequisite for immediate preventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/544
Inventor 易丁萍
Owner GUANGZHOU YIXI DRINK
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