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Capillary electrophoresis method for detecting medications and poisons in blood and urine at the same time

A capillary electrophoresis and poison technology, which is used in the preparation of test samples, measuring devices, color/spectral characteristics measurement, etc. Simple, easy to operate, strong detection specificity

Inactive Publication Date: 2007-02-21
上海市公安局刑事侦查总队
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its lack of versatility, the practicability and comparability of unknown drug toxicity analysis are poor

Method used

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  • Capillary electrophoresis method for detecting medications and poisons in blood and urine at the same time
  • Capillary electrophoresis method for detecting medications and poisons in blood and urine at the same time
  • Capillary electrophoresis method for detecting medications and poisons in blood and urine at the same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1 material

[0053] 1.1 Instruments Beckman P / ACE MDQ capillary electrophoresis instrument (Beckman Company, USA); uncoated quartz capillary column (75μm×50.2cm, effective separation length is 40cm, Beckman Company); oasis HLB column (1mL / 30mg, waters company); Vortex mixer XW-80A; Electric constant temperature water bath.

[0054] 1.2 Reagents and medicines

[0055] Opioids and their synthetics: Pholcodine, Thebaine, Codeine Phosphate, Morphine Sulfate, O 6 - Monoacetylmorphine (O 6 -monoacetylmorphine), Heroin Hydrochloride, Noscapine, Pethidine Hydrochloride, Methadone Hydrochloride, Acetylcodeine, O 3 - Monoacetylmorphine (O 3 -monoacetylmorphine), Fentanyl Citrate, Papaverine Hydrochloride, Diphenoxylate Hydrochloride, Buprenorphine Hydrochloride, Naloxone Hydrochloride.

[0056] Cocaine and synthetic substitutes: Cocaine Hydrochloride, Tetracaine, Procaine Hydrochloride, Lidocaine, Bupivacaine.

[0057] Amphetamines and blends: Amphetamine Sulfate, Methamphe...

Embodiment 2

[0141] Example 2 Drug analysis in the urine sample of the person taking ecstasy

[0142] Source of urine sample: Shanghai Drug Testing Center, the appearance is clear.

[0143] The blank urine sample and other medicines, reagents and used instrument materials are the same as those in Example 1.

[0144] Working curve: with embodiment 1.

[0145] Sample processing: with embodiment 1.

[0146] The instrument conditions are the same as in Example 1.

[0147] MDA was detected in the urine sample of the suspect taking ecstasy, with a concentration of 4120ng·mL -1 . Chromatogram see Figure 6 .

Embodiment 3

[0148] Example 3 Analysis of Drugs in the Urine Samples of Drug Addicts

[0149] Source of urine sample: Shanghai Drug Testing Center, the appearance is clear.

[0150] The blank urine sample and other medicines, reagents and used instrument materials are the same as those in Example 1.

[0151] Working curve: with embodiment 1.

[0152] Sample processing: with embodiment 1.

[0153] The instrument conditions are the same as in Example 1.

[0154]The results showed that the MDMA content in the urine sample exceeded the range of the working curve. In order to obtain correct quantitative data, the urine sample was diluted 5 times with water and measured again. Methamphetamine, MDMA and ketamine were detected in the urine samples of drug addicts , the concentrations were 2917ng·mL -1 , 8512ng·mL -1 and 887ng·mL -1 . Chromatogram see Figure 7 .

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Abstract

A capillary cataphoresis method for detecting multiple medicinal toxic in blood and in urine simultaneously includes carrying out pretreatment by using similar solid phase to extract basic and acidic medicinal toxic, applying high efficiency of capillary cataphoresis detection and internal standard means of quantitative for enabling to analyze out 55 types of medicinal toxics contained possibly in one blood sample or in one urine sample within dozens of ten minutes.

Description

technical field [0001] The present invention relates to a method for simultaneously detecting multiple drugs and poisons in blood or urine, and more particularly relates to a high-performance capillary electrophoresis (HPCE) method capable of simultaneously quantitatively determining 55 drugs and poisons in blood or urine . Background technique [0002] The main methods currently used in the analysis of drugs and toxicants are: gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC) and enzyme-linked immunoassay ( EMIT) and so on. The application of HPCE to the analysis of drugs and poisons has developed rapidly in recent years. Domestic and foreign literatures have reported the application of HPCE in poison analysis, but they mainly focus on the research of the same kind of drug poison or the mixture of a few varieties. Zhang Yahai et al. used HPCE to detect the content of morph...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/34G01N27/447
Inventor 张玉荣梁晨汪蓉金琦芸张润生龚飞君
Owner 上海市公安局刑事侦查总队
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