Quick joint inspection test strip for Marek disease virus (MDV) and reticuloendotheliosis virus (REV)

Abstract

The invention discloses a test strip for quickly detecting Marek disease virus (MDV) and reticuloendotheliosis virus (REV) through a one-step method. The test strip comprises a water repellent supporting layer and an adsorbed layer adhered to the supporting layer, wherein the adsorbed layer is composed of a sample adsorption fiber layer, a gold-labeled antibody fiber layer, a cellulose membrane layer and a water accepting layer, which are spliced in sequence; controlled imprinting of anti-goat IgG or anti-mouse IgG and antibody detection imprinting containing anti-MDV and anti-REV are marked on the cellulose membrane layer; an antibody of the anti-MDV and anti-REV is a monoclonal antibody or a polyclonal antibody of the anti-MDV and anti-REV; and a mixed polyclonal antibody or a monoclonal antibody of the anti-MDV and anti-REV corresponding to the detection imprinting and marked by colloidal gold is adhered to the gold-labeled antibody fiber layer. The test strip is high in detection specificity, high in sensitivity, simple and convenient to operate, quick to detect and intuitive in result, so that the test strip is suitable for onsite quick joint inspection on MDV and REV and can be also applied to differential diagnosis on separate infection or mixed infection of the two viruses. The test strip has a wide application range and is convenient to generalize and apply.

Description

[0001] technical field [0002] The invention relates to a device for detecting Marek's disease and poultry reticuloendotheliosis, in particular to a colloidal gold test strip for rapid detection of Marek's disease virus and poultry reticuloendotheliosis virus in one step. technical background [0003] Marek's disease (Marek's disease, MD) is a highly contagious, contagious, lymphoproliferative neoplastic disease of poultry caused by Marek's disease virus (MDV), mainly harming chickens, quail and pheasant also sickness. The disease can cause neuroswelling of lymphocytomas of internal organs, muscles, and peripheral nerves. Clinically, MD-affected chicken flocks show symptoms such as uneven growth, extreme emaciation, and death. The most common lesions are nerve damage and lymphatic tumors of various solid organs, with liver and spleen tumors being the most common. Asymmetric progressive paralysis, eventually leading to complete paralysis of one or more limbs. MDV belongs ...

AI Technical Summary

Problems solved by technology

The detection cost of this method is relatively high, and because the REV infection in some cases is "transient", the viremia time is short, and the virus content is low, the detection rate is low

Molecular biology methods such as PCR, fluorescent quantitative PCR and LAMP are mainly used for the detection of viral nucleic acids. Although these technologies can accurately differentiate MDV and REV, the operation is complicated and requires specific reagents and expensive equipment. The operation requirements are high, and it is difficult to meet the needs of rapid differential diagnosis on the production line or on the scene of epidemic diseases

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