Electrochemical luminescence adapter sensor as well as preparation method and usage thereof

An aptamer sensor, electrochemical technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., to achieve the effects of long luminescence lifetime, high quantum yield, and high sensitivity

Active Publication Date: 2015-02-25
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the development of carbon nanomaterials with both carrier function and luminescent function is of great significance in life science and material sc

Method used

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  • Electrochemical luminescence adapter sensor as well as preparation method and usage thereof

Examples

Experimental program
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Example Embodiment

[0038] Example 1 Preparation of electrochemiluminescence aptamer sensor

[0039] (1) Synthesis of Eu(III)-MWCNTs nanomaterials

[0040] Rare earth complex Eu(TTA) 3 Synthesis of: Mix 9 mL of ethanol solution containing 1.0 mol / L KOH and 1.28 g (3 mmol) of europium nitrate pentahydrate, then add 15 mL of ethanol and 2 g of 2-thenoyl trifluoroacetone (9 mmol), The mixed solution was heated to 60 ℃ under stirring for 6 h. After cooling to room temperature, add water and continue stirring for 30 min. The obtained solid was recrystallized with ethanol, dried in the air and dried in a phosphorus pentoxide dryer for 24 hours to obtain a white rare earth complex Eu(TTA) 3 ;

[0041] Synthesis of Eu(III)-MWCNTs: Mix 100 mg of carboxylated multi-walled carbon nanotubes, 20 mL of anhydrous dichloromethane (DCM) and 0.5 mL of oxalyl chloride in a 50 mL round bottom flask. After stirring for 12 h at room temperature, DCM was removed by distillation under reduced pressure to obtain acid chlorid...

Example Embodiment

[0052] Example 2 Detection of Thrombin

[0053] The electrochemiluminescence aptamer sensor was prepared according to the steps described in Example 1 for the detection of thrombin. The linear range of thrombin is 5×10 -9 ~1.0×10 -12 mol / L, the detection limit is 0.23 pmol / L.

[0054] The results obtained in Example 2 were compared with the literature. Through comparison, it can be found that the electrochemiluminescence aptamer sensor of the present invention has a lower detection limit, a wider linear range, and a higher sensitivity, which lays a foundation for the actual determination of thrombin.

Example Embodiment

[0055] Example 3 Detection of Thrombin in Human Serum

[0056] The thrombin in human serum was measured and spiked and recovered experiments. According to the working curve obtained in Example 2, the content of thrombin in the serum was calculated. The three determination results showed that the three determination results of human blood samples were 0.589, 0.618, 0.603 nmol / L, and the average thrombin content was 0.603 nmol / L. At the same time, the standard addition method was adopted, and the addition amount of thrombin was 0.500 nmol / L, and the average recovery rate was 96.8%, and the relative standard deviation was 2.4%. It shows that the electrochemiluminescence aptamer sensor obtained by the preparation method of the present invention has high accuracy and precision.

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Abstract

The invention relates to an electrochemical luminescence adapter sensor as well as a preparation method and the usage thereof, and in particular relates to an electrochemical luminescence adapter sensor established by taking Eu (III)-MWCNTs as luminescent material, a preparation method of the electrochemical luminescence adapter sensor, and the usage of the electrochemical luminescence adapter sensor prepared by the method in measurement of thrombin. The electrochemical luminescence adapter sensor has the advantages of excellent accuracy, stability and reproducibility and high sensitivity and has an important significance in the popularization of practical application of the adapter sensor in disease prevention, disease diagnosis and drug screening.

Description

technical field [0001] The present invention relates to an electrochemiluminescent aptasensor, its preparation method and its application, more specifically, the present invention relates to an electrochemiluminescent aptasensor based on Eu(III)-multi-walled carbon nanotubes (MWCNTs) as a luminescent material. A chemiluminescence aptasensor, a preparation method thereof, and an application of the electrochemiluminescence aptasensor prepared by the method in measuring thrombin. Background technique [0002] Thrombin is a serine proteolytic enzyme that is activated by prothrombin. It plays an extremely important role in inflammation, blood coagulation and wound healing, and is often used for local hemostasis of capillary bleeding and tissue healing after surgery. The concentration and activity of thrombin, as one of the important indicators to measure the coagulation mechanism, is of great significance for early diagnosis, curative effect and prognosis judgment. Therefore, i...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 吴丹魏琴胡丽华庞雪辉马洪敏
Owner UNIV OF JINAN
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