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Electrochemical luminescence adapter sensor as well as preparation method and usage thereof

An aptamer sensor, electrochemical technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., to achieve the effects of long luminescence lifetime, high quantum yield, and high sensitivity

Active Publication Date: 2015-02-25
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the development of carbon nanomaterials with both carrier function and luminescent function is of great significance in life science and material science research, and Eu(III)-MWCNTs are used as luminescent materials to construct electrochemiluminescence aptasensors for detecting thrombin Not yet reported

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  • Electrochemical luminescence adapter sensor as well as preparation method and usage thereof

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Effect test

Embodiment 1

[0038] Example 1 Preparation of Electrochemiluminescence Aptasensor

[0039] (1) Synthesis of Eu(III)-MWCNTs nanomaterials

[0040] Rare earth complex Eu(TTA) 3 Synthesis of : Mix 9 mL of ethanol solution containing 1.0 mol / L KOH and 1.28 g (3 mmol) of europium nitrate pentahydrate, then add 15 mL of ethanol and 2 g of 2-thienoyltrifluoroacetone (9 mmol), The mixed solution was heated to 60 °C for 6 h under stirring. After cooling to room temperature, add water and continue to stir for 30 min. The obtained solid is recrystallized with ethanol, dried in air and then placed in a phosphorus pentoxide dryer for 24 h to obtain the white rare earth complex Eu(TTA) 3 ;

[0041] Synthesis of Eu(III)-MWCNTs: 100 mg of carboxylated multi-walled carbon nanotubes, 20 mL of anhydrous dichloromethane (DCM) and 0.5 mL of oxalyl chloride were mixed in a 50 mL round bottom flask After stirring at room temperature for 12 h, acid chloride-functionalized MWCNTs were obtained after removal o...

Embodiment 2

[0052] Example 2 Detection of thrombin

[0053] According to the steps described in Example 1, an electrochemiluminescence aptasensor was prepared for the detection of thrombin. Thrombin has a linear range of 5 × 10 -9 ~1.0×10 -12 mol / L, the detection limit is 0.23 pmol / L.

[0054] The results obtained in Example 2 were compared with the literature. Through comparison, it can be found that the detection limit of the electrochemiluminescent aptasensor of the present invention is low, the linear range is wide, and the sensitivity is high, which lays the foundation for the actual determination of thrombin.

Embodiment 3

[0055] The detection of thrombin in embodiment 3 human serum

[0056] The thrombin in human serum was measured and the standard addition recovery experiment was carried out, and the content of thrombin in serum was calculated according to the working curve obtained in Example 2. The results of three measurements showed that the three measurements of human blood samples were 0.589, 0.618, and 0.603 nmol / L, respectively, and the average thrombin content was 0.603 nmol / L. At the same time, the standard addition method was adopted, and the amount of thrombin added was 0.500 nmol / L. The average recovery rate was 96.8%, and the relative standard deviation was 2.4%. It shows that the electrochemiluminescence aptasensor obtained by the preparation method of the present invention has high accuracy and precision.

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Abstract

The invention relates to an electrochemical luminescence adapter sensor as well as a preparation method and the usage thereof, and in particular relates to an electrochemical luminescence adapter sensor established by taking Eu (III)-MWCNTs as luminescent material, a preparation method of the electrochemical luminescence adapter sensor, and the usage of the electrochemical luminescence adapter sensor prepared by the method in measurement of thrombin. The electrochemical luminescence adapter sensor has the advantages of excellent accuracy, stability and reproducibility and high sensitivity and has an important significance in the popularization of practical application of the adapter sensor in disease prevention, disease diagnosis and drug screening.

Description

technical field [0001] The present invention relates to an electrochemiluminescent aptasensor, its preparation method and its application, more specifically, the present invention relates to an electrochemiluminescent aptasensor based on Eu(III)-multi-walled carbon nanotubes (MWCNTs) as a luminescent material. A chemiluminescence aptasensor, a preparation method thereof, and an application of the electrochemiluminescence aptasensor prepared by the method in measuring thrombin. Background technique [0002] Thrombin is a serine proteolytic enzyme that is activated by prothrombin. It plays an extremely important role in inflammation, blood coagulation and wound healing, and is often used for local hemostasis of capillary bleeding and tissue healing after surgery. The concentration and activity of thrombin, as one of the important indicators to measure the coagulation mechanism, is of great significance for early diagnosis, curative effect and prognosis judgment. Therefore, i...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 吴丹魏琴胡丽华庞雪辉马洪敏
Owner UNIV OF JINAN
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