Antibody chip kit for early screening and diagnosis of tumors

Abstract

The invention discloses an antibody chip kit for early screening and diagnosis of tumors. The detection is realized based on an enzyme lined immunosorbent assay (ELISA); the antibody chip kit comprises an antibody chip and an antigen standard substance mixture of multiple tumor markers, wherein the antibody chip comprises a base membrane and capture antibodies, fixed on the base membrane, of the multiple tumor markers; the base membrane is a polydimethylsiloxane (PDMS) membrane having a surface containing hydrophilic groups. On the premise of maintaining the characteristics of high sensitivity, large flux, much information and the like, the base membrane adopted by the invention is good in stability, strong in biological compatibility, good in flexibility, easy to clean and convenient to use; chip substrates of the capture antibodies of the tumor markers are fixed.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an antibody chip kit for early screening and diagnosis of tumors. The invention is especially suitable for early screening and diagnosis of various tumors. Background technique [0002] In recent years, malignant tumors have become one of the major diseases that endanger human health and threaten human life. As a disease with high morbidity and mortality, early screening and diagnosis of tumors are of great significance to prevent and treat tumors and improve prognosis. However, most tumors often have no obvious symptoms in the early stage, and imaging examinations, pathological examinations, and endoscopic examinations cannot be diagnosed in time, and tumor marker detection can screen and assist in the early diagnosis of tumors. [0003] Tumor markers refer to a class of substances expressed and secreted by tumor cells during the occurrence and proliferation of malignant ...

AI Technical Summary

Problems solved by technology

However, the carrier system of these products is based on the substrate or glass slide of nitrocellulose membrane. Nitrocellulose membrane is a porous material with relatively poor mechanical strength. Membrane rupture is prone to occur during the operation of the kit, resulting in contamination between samples. ; and the protein non-specific adsorption of nitrocellulose membrane is strong, the background is high, and the blocking and washing process of the active site is required.

The glass slide is relatively brittle and easy to crush, which is not conducive to the fixation of the antibody chip substrate.

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