(Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow

A technology of Bossia spp. and jiji mushrooms, applied in the biological field of edible fungi, can solve problems such as low yield and environmental pollution, and achieve the effects of increasing yield, increasing growth rate, and shortening the production cycle

Active Publication Date: 2020-01-14
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the technical problem to be solved by this invention is to provide a kind of Bossia Y4, which has a promoting effect on the growth of Shimeji mushroom; the technical problem to be solved by this invention is to provide Bossella Y4 Application in promoting the growth of Shiji mushroom to solve the problems of low yield and environmental pollution of traditional Shiji mushroom planting methods

Method used

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  • (Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow
  • (Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow
  • (Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 1) Isolation, screening and identification of bacteria

[0021] The cultivation material of Pleurotus jijiji mushroom sticks was randomly collected from Shandong Changshengyuan Edible Mushroom Co., Ltd., and 42 bacterial isolates were isolated from CCP by gradient dilution method and diluted coating with enriched medium plates. Then use NA medium for separation and purification.

[0022] Among them, the formula of NA medium is peptone 10g, beef extract 5g, sodium chloride 5g, agar 20g, pH7.2~7.4.

[0023] Put the strains preserved after primary screening, isolation and purification into conical flasks of NA liquid medium, and culture them on a shaker at 28°C and 180r / min for 48 hours to obtain saturated bacterial liquids, and perform aseptic operation with a pipette gun It is required to inoculate 0.2mL of each bacterial solution on enrichment plates (glucose 20g, potato 200g, peptone 10g, yeast extract 3g, agar 15-20g, water 1000mL, pH7.0), spread evenly with a spread...

Embodiment 2

[0028] Effect of Bossia sp. Y4 on fruiting

[0029] In the workshop of the school’s cooperative edible fungi enterprise, 4000 bottles of shimeji mushrooms were cultivated and produced according to the normal production standards of the enterprise, of which 2000 bottles were inoculated with fungicides, and the concentration of each bottle was 5×10 7 ~1×10 8 CFU / mL of Bossia Y4 5mL; at the same time, 2000 bottles of sterile water with the same total volume were sprayed as a control to observe the fruiting speed and yield of Shiji mushroom. Effects of growth-promoting bacteria of edible fungi on the fruiting body traits of Shiji mushroom strains are as follows: Figure 2 to Figure 6 shown by figure 2 It can be seen that the diameter of the cap of the fruiting bodies of jiji mushrooms added with growth-promoting bacteria becomes larger, which is determined by image 3 It can be obtained that the height of the stipe of the fruiting bodies of jiji mushrooms added with growth-pro...

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Abstract

The invention discloses (Bosea sp.) Y4 and application thereof for accelerating Hypsizygus marmoreus(Peck)H .E .Bigelow to grow, and relates to the biotechnology field of edible fungi. The (Bosea sp.)Y4 is collected in the China Center for Type Culture Collection with a collection number CCTCC NO:M 2019634, wherein a collection date is 16th, August, 2019, and a collection address is Wuhan University, China. The (Bosea sp.) Y4 is used for accelerating the Hypsizygus marmoreus(Peck)H .E .Bigelow to grow. Through bacteria solution culture, cultivation application and fruiting management, the Hypsizygus marmoreus(Peck)H .E .Bigelow in which the (Bosea sp.) Y4 is added is characterized in that a hypha growth rate is increased, the diameter of a sporocarp pileus is increased, a stipe is long, time for a bottle to be full is shortened and a bottle yield is increased. The growth rate of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be effectively improved, the production period of the Hypsizygus marmoreus(Peck)H .E .Bigelow is shortened, the yield of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved, a product is guaranteed to be environmentally-friendly, healthy and free frompollution, and the production efficiency and the social benefit of the Hypsizygus marmoreus(Peck)H .E .Bigelow can be improved.

Description

technical field [0001] The invention relates to the field of edible fungus biotechnology, in particular to Bossia genus Y4 and its application in promoting the growth of shimeji mushroom. Background technique [0002] Hypsizygus marmoreus (Peck) H.E.Bigelow, also known as jade mushroom, is of the genus Basidiomycotina, Laminomycetes, Agaricaceae, White Mushrooms, and Hypsizygus marmoreus. Delicious, with sea crab flavor. Fruiting bodies are medium to large. The amino acids in the protein of Jasmine mushrooms are complete, including 8 kinds of essential amino acids for human body, among which the content of lysine and arginine are higher than that of common mushrooms, which play an important role in the growth of teenagers' intelligence and growth. The β-1, 3-D glucan extracted from the fruiting bodies of Japonica japonica has very high anti-tumor activity, and the activity of the polymerase isolated from Japonia japonica is much higher than that of other mushrooms. Fruiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G18/20C12R1/01
CPCC12N1/20A01G18/20C12R2001/01C12N1/205
Inventor 李冠喜焦玉茹张璐于颖媛李秋月张雯倩卢茹杰郭帅
Owner QUFU NORMAL UNIV
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