Method for detecting neutral triterpenes in ganoderma fungus

A neutral and fungal technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that there is no detection method for neutral triterpenes, the maximum ultraviolet absorption of compounds is inconsistent, and it is not comprehensive and objective.

Pending Publication Date: 2022-01-11
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the complex structure of neutral triterpenes, the maximum UV absorption of each compound is inconsistent, and it is difficult to accurately detect through UV absorption. Therefore, there has not been a more comprehensive detection method for neutral triterpenes.
However,

Method used

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  • Method for detecting neutral triterpenes in ganoderma fungus
  • Method for detecting neutral triterpenes in ganoderma fungus
  • Method for detecting neutral triterpenes in ganoderma fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The establishment of embodiment 1 detection method

[0062] 1. The pretreatment step of Ganoderma lucidum fruiting body: Accurately weigh 6.56g of dried Ganoderma lucidum fruiting body samples (fruiting bodies 1, 2, 3, 4, 5) in a stoppered test tube, according to 1:36 (weight to volume ratio, weight g: volume ml) of solid-liquid ratio Add methanol 15mL, after ultrasonic extraction for 66min, take the supernatant, pass through a 6.26μm organic filter membrane and dilute 26 times (volume multiple) with mass spectrometry grade methanol to obtain the sample solution to be tested for loading ;

[0063] Preparation of mixed standard solution: Prepare mixed standard solution mother solution with mass spectrometry grade methanol (the concentration of compound 1 is 266 μg / mL; the concentration of compound 2, 3, 4, 5, 7, 8 is 166 μg / mL; the concentration of compound 6 is 56 μg / mL; the concentration of compounds 9, 10, and 12 is 25 μg / mL, and the concentration of compound 11 is ...

Embodiment 2

[0086] Methodology validation and result testing:

[0087] 1. Precision: Take the mixed standard solution with the highest concentration standard diluted 4 times and repeat the injection 6 times in the same day, calculate the concentration of triterpenes obtained in 6 experiments according to the standard curve, and calculate the intraday precision. Take the mixed standard solution of the highest concentration standard solution and dilute it step by step 4 times for three consecutive days, inject samples twice a day, and calculate the inter-day precision based on the results of 6 experiments. The results showed that the RSDs of the intra-day precision and inter-day precision of the 12 triterpenoids were less than 15.66%, which indicated that the method had good intra-day and inter-day precision. The specific results are shown in Table 2.

[0088] 2. Stability: take the fruiting body 5, extract according to the sample pretreatment step in Example 1, prepare a portion of the sa...

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Abstract

The invention provides a method for detecting neutral triterpenes in ganoderma fungi. The method comprises the following steps: (1) pretreating ganoderma lucidum sporocarp; (2) performing an ultra-high performance liquid chromatography detection; (3) obtaining mass spectrum information of the compound; (4) establishing an ultra-high performance liquid chromatography-triple quadrupole mass spectrometry combined analysis method; (5) performing data analysis. According to the method for simultaneously detecting the 12 neutral triterpenoids in the ganoderma fungus, the 12 neutral triterpenoids in the ganoderma fungus are qualitatively and quantitatively analyzed simultaneously, and accurate and reliable detection analysis is provided for related research of the ganoderma fungus.

Description

technical field [0001] The invention relates to the field of food and drug analysis, in particular to a method for detecting neutral triterpenes of fungi belonging to the genus Ganoderma lucidum. Background technique [0002] Ganoderma P.Karst belongs to Basidiomycota, Agaricomycetes, Polyporales, and Ganodermataceae. It is an important group of higher basidiomycetes, with a total of 137 species. . Among them, Ganoderma lucidum and Ganoderma lucidum (Ganoderma tsugae) are stipulated by the Chinese Pharmacopoeia to be used as medicinal materials. Together, Ganoderma lucidum and Ganoderma tsugae can be used as raw materials for health food development. Ganoderma lucidum contains a variety of triterpenoids, which are one of the main active ingredients and have various physiological activities. These triterpenes can be divided into two categories by acid-base extraction, namely acid triterpenes and neutral triterpenes. Acidic triterpenes are mainly Ganoderic acid, Ganoderic a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 冯娜张劲松程池露王晨光唐庆九周帅冯杰王金艳周靖
Owner SHANGHAI ACAD OF AGRI SCI
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