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Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same

A technology of artificial cultivation and breeding methods, applied in the direction of botany equipment and methods, fertilizer mixtures, gardening, etc.

Inactive Publication Date: 2010-05-19
徐正华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no live Cordyceps that is completely hosted by insect larvae, grows 1-2 roots on the head, and is more than 2cm long, similar to wild Cordyceps.

Method used

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  • Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same
  • Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same
  • Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 cultivates Cordyceps militaris

[0071] Prepare every 1000ml culture medium according to the proportion of components. Among them, glucose 2% (w / v), potassium dihydrogen phosphate 0.2% (w / v), magnesium sulfate 0.15% (w / v), milk powder 0.5% (w / v) (Shanghai Guangming Dairy Co., Ltd.), beef Extract 1% (w / v) (Invitrogen Company), potato filtrate 200ml and 2 tablets of complex vitamin B (Shanghai Jiufu Pharmaceutical Co., Ltd.).

[0072] Add 200g of potatoes to every 1000ml of water, boil for half an hour and filter to obtain potato filtrate.

[0073] Cordyceps militaris (Shanghai Academy of Agricultural Sciences) was inoculated into the high-temperature sterilized Cordyceps militaris culture solution, and cultured in shake flasks at 20-30° C. for 5-7 days to obtain the Cordyceps militaris bacteria solution.

Embodiment 2

[0074] Embodiment 2 Cordyceps militaris infects live barley worm larvae

[0075] 88% (w / w) wheat bran, 10% (w / w) corn flour, 2% (w / w) Cordyceps militaris bacterium liquid (cultivated in Example 1) and every kilogram of Cordyceps militaris bacterium liquid drop into 10 tablets of vitamin B complex formulated as live barley worm larvae feed.

[0076] At a temperature of 22-30° C. and a relative humidity of 60-70%, the feed was fed to barley larvae (South China Rare Insect Breeding Research Center) for 16 days. Afterwards, the active barley worm larvae were sprayed with the Cordyceps militaris bacterial solution cultivated in Example 1, so that the Cordyceps militaris infects the larvae.

Embodiment 3

[0077] Embodiment 3 Cordyceps militaris infects live barley larvae

[0078] 77% (w / w) wheat bran, 15% (w / w) corn flour, 8% (w / w) Cordyceps militaris bacterium liquid (cultivated in Example 1) and every kilogram of Cordyceps militaris bacterium liquid drop into 10 tablets of vitamin B complex formulated as live barley worm larvae feed.

[0079] At a temperature of 22-30° C. and a relative humidity of 60-70%, the feed was fed to barley larvae for 25 days. Afterwards, the active barley worm larvae were sprayed with the Cordyceps militaris bacterial solution cultivated in Example 1, so that the Cordyceps militaris infects the larvae.

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Abstract

The invention provides a living Chinese caterpillar fungi artificially cultivated by using larvae as hosts, which comprise insect larvae and one or two about two centimeter living Chinese caterpillar fungus fruiting bodies grown on the head of each the larva. The cultivating process comprises the steps of: invasion of the Chinese caterpillar fungi into the living insect larvae, in vivo growth of the hyphae in the living insect larvae, inactivation of the larvae, growth of the Chinese caterpillar fungus fruiting bodies on the heads and the like; and in the whole process, not only the insect larvae and the hyphae are grown in the polypide, but also the Chinese caterpillar fungus fruiting bodies are grown on the heads, the obtained Chinese caterpillar fungi have the same appearance and almost the same nutrient content as the wild Chinese caterpillar fungi, so that the Chinese caterpillar fungi obtained by the method are the real living Chinese caterpillar fungi obtained by artificial cultivation.

Description

technical field [0001] The invention relates to an artificially cultivated live cordyceps, in particular to an artificially cultivated live cordyceps with larvae as hosts and a cultivation method thereof. Background technique [0002] Cordyceps sinensis (Cordyceps Sinensis (Berk.) Sacc.), also known as Cordyceps sinensis and Cordyceps sinensis, is a complex of fruiting bodies of Cordyceps sinensis fungi and larval rigid bodies formed by the ergot fungus Cordyceps sinensis parasitizing on the larvae of the host bat moth insects. In midsummer, on high-altitude forest meadows or lawns, bat moths leave their eggs on flowers and leaves. After the moth eggs become adults, they burrow into the moist and loose soil to absorb the nutrient growth of plant roots. The spherical ascospores will infect the larvae of the Cordyceps bat moth, and germinate hyphae in the larvae. In addition, when the larvae of the Cordyceps bat moth eat the leaves with the Cordyceps fungus, they may also bec...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00C05G3/00
Inventor 徐正华王才根
Owner 徐正华
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