Method for producing cordyceps militaris sporocarp and fish feed additive at same time

A feed additive and fish feed technology, applied in botany equipment and methods, animal feed, gardening methods, etc., can solve the problems of high cost and poor disease resistance of prawns, increase the weight gain rate, reduce the cost of breeding, and feed The effect of coefficient reduction

Active Publication Date: 2018-01-26
LUDONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to provide a kind of culture medium formula for producing Cordyceps militaris and feed additive and preparation method thereof with the fungus slag of

Method used

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  • Method for producing cordyceps militaris sporocarp and fish feed additive at same time
  • Method for producing cordyceps militaris sporocarp and fish feed additive at same time

Examples

Experimental program
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Embodiment 1

[0045] Embodiment 1: Preparation of Cordyceps militaris culture medium.

[0046] The formula of Cordyceps militaris culture medium is divided into two components: A liquid and B solid. 2 PO 4 0.3~0.5 g, MgSO 4 0.3-0.5 g, 4 tablets of complex vitamin B, 1 tablet of vitamin C. The composition of B solid part is as follows: wheat grain 50%, rapeseed meal 15%, peanut meal 24%, dry silkworm chrysalis powder 1%, rice bran 10%; The liquid-solid ratio of A liquid and B solid is 2~3: 1; And fully Mix well, put into culture bottle, seal, sterilize and cool. The sterilization conditions are: 121°C, 1.1 MPa for 50-60 minutes. The Cordyceps militaris culture medium provided by the present invention can be used for cultivating Cordyceps militaris.

Embodiment 2

[0047] Embodiment 2: the cultivation of Cordyceps militaris.

[0048] Cultivate Cordyceps militaris using the Cordyceps militaris medium prepared in the examples, including inoculation, cultivation, harvesting, drying and other steps. Specifically: vaccination first. Inoculate the Cordyceps militaris bacterial classification in the culture bottle of the prepared Cordyceps militaris culture medium of embodiment 1 in ultra-clean bench, described Cordyceps militaris bacterial classification is Cordyceps militarys (L.) Link., liquid bacterial classification, described Cordyceps militaris bacterial classification The density of the species is 3 to 5 bacterial balls / mL, 1ml per culture bottle; further culture: at a temperature of 25°C and a humidity of 80 to 87%, culture in the dark for 5 to 6 days until the mycelium of Cordyceps militaris is overgrown. Describe the surface of the Cordyceps militaris culture medium; the light culture adopted in the 6th to 25th day: daytime 12 hours...

Embodiment 3

[0055] Embodiment 3: A kind of feed formulation and its preparation method using Cordyceps militaris culture medium residue as raw material.

[0056] The invention discloses that it solves the problems that the existing feed cost is high and the quality of prawns cannot be improved well. The prawn feed additive of the present invention is composed of fungus slag powder of Cordyceps militaris culture medium, which contains Cordyceps mycelium powder, unused rapeseed meal, peanut meal and rice bran, vitamin mixture, trace elements and the like. The components of the present invention utilize production wastes such as solid medium residues remaining after harvesting the fruiting body of Cordyceps militaris, fully recycle the production wastes, and greatly reduce the production cost of the feed.

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Abstract

The invention discloses a method for producing cordyceps militaris sporocarp and a fish feed additive at the same time. A culture medium formula for producing the cordyceps militaris sporocarp comprises liquid A and solid B; the liquid A is prepared from the following components of 1000ml of water, 10.0 to 15.0g of saccharose, 0.3 to 0.5g of KH2PO4, 0.3 to 0.5g of MgSO4, four tablets of complex vitamin B and 1 tablet of vitamin C; the solid B is prepared from the following materials of 50% of wheat berry, 15% of rapeseed meal, 24% of peanut meal, 1% of dry silkworm chrysalis meal and 10% of rice bran; a ratio of the liquid A to the solid B powder is (2 to 3) to 1. The method comprises the steps of sealing, sterilizing and cooling the culture medium; then inoculating cordyceps militaris strain, inoculating according to a volume ratio of the strain to the culture medium as 1 to 50 and performing dark culture at first and then performing light culture in cultivation, wherein a strain density is 10 to 20 fungus balls/mL; obtaining the sporocarp and fungi residues. A crude polysaccharide content of the fungi residues reaches 27.43%, the fungi residues are naturally dried or stoved and then smashed to 80-mesh granularity, and digestion and absorption of fish bodies are facilitated; furthermore, the fungi residues have fragrant smell and can serve as the fish feed addition, and an adding amount is 10 to 15%.

Description

technical field [0001] The invention relates to the cultivation technology of Cordyceps militaris and the field of production of fish feed additives, in particular to a formulation of a medium for Cordyceps militaris and the application of the fungus residue after producing the fruiting bodies of Cordyceps militaris in fish feed and feed additives. Background technique [0002] Cordyceps militaris ( Cordyceps militaris ), also known as Cordyceps militaris, belongs to the Ascomycotina, Ergotaceae, and Cordyceps of the fungal kingdom. The fruiting body of Cordyceps militaris has similar medicinal and nourishing effects to Cordyceps sinensis, and the content of cordycepin in Cordyceps militaris is dozens of times higher than that of traditional Cordyceps sinensis. It is a precious medicinal fungus. Cordyceps militaris is rich in protein, amino acid and more than 30 kinds of trace elements needed by the human body. It also contains unique physiologically active components such ...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G7/04A23K50/80A23K20/163A23K20/26A23K20/174A23K10/30A23K10/20A23K10/37C05G1/00
CPCY02A40/818Y02P60/87
Inventor 张秋胜徐丙莲程显好潘佳佳李田招
Owner LUDONG UNIVERSITY
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