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Rapid preparation method of termitomyces robustus strain

A production method and technology of fir fir are applied in the directions of botanical equipment and methods, fertilizer mixtures, horticulture, etc., and can solve the problems of difficulty in propagation, slow growth of mycelium, difficulty in mass production, etc., and achieve shortened cultivation period and low production cost. , the effect of simple culture medium

Inactive Publication Date: 2012-06-13
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still many problems in the research of artificial cultivation. Among them, the mycelium grows extremely slowly on the artificial medium, it is difficult to multiply, and it is difficult to produce bacteria in batches. It is still a major problem, which is the premise or basis for restricting or affecting artificial cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Mature fruiting bodies picked in the field with good growth, no pests, unopened umbrellas, thicker stipe, and thicker caps;

[0019] Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into two from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.20-0.40cm 2 small pieces;

[0020] Gently embed the above-cut small tissue pieces on the surface of the mother seed medium on the inclined plane of the test tube. The mother seed medium is: potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.35g / L, KNO 3 0.35g / L, vitamin B 1 2.50mg / L, agar 12.00g / L, control pH value 4.5, culture temperature 26°C, dark culture for 15 days, a small amount of white hyphae sprouted on the o...

example 2

[0024] Mature fruiting bodies picked in the field with good growth, no pests, unopened umbrellas, thicker stipe, and thicker caps;

[0025] Bring the fruiting bodies back to the laboratory, use alcohol cotton balls on the ultra-clean table, lightly wipe the surface of the cap and the stipe and other parts, remove the surface sediment or soil, divide the fruiting bodies from the middle of the cap in two, and dissect Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.20-0.40cm 2 small pieces;

[0026] Gently embed the above-cut small tissue pieces on the surface of the mother seed medium on the inclined plane of the test tube. The mother seed medium is: potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 0.50g / L, KH 2 PO 4 0.60g / L, NH 4 NO 3 0.45g / L, KNO 3 0.45g / L, vitamin B 12.00mg / L, agar 12.00g / L, control pH value 4.5, culture temperature 25°C, dark culture for 18 days, a small amount of white hyphae...

example 3

[0030] Mature fruiting bodies picked in the field with good growth, no pests, unopened umbrellas, thicker stipe, and thicker caps;

[0031] Bring the fruiting body back to the laboratory, wipe the surface of the cap and the stipe with an alcohol cotton ball on the ultra-clean table to remove the surface sediment or soil, divide the fruiting body into two from the middle of the cap, and dissect it Take the internal tissue block at the junction of the stipe and the cap with a knife, and cut into about 0.20-0.40cm 2 small pieces;

[0032] Gently embed the above-cut small tissue pieces on the surface of the mother seed medium on the inclined plane of the test tube. The mother seed medium is: potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 0.80g / L, KH 2 PO 4 0.40g / L, NH 4 NO 3 0.40g / L, KNO 3 0.40g / L, vitamin B 1 2.20mg / L, agar 12.00g / L, control PH value to 4.0, culture temperature is 24°C, culture in dark for 17 days, a small amount of white hyphae sprouted...

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Abstract

The invention relates to a strain preparation method of rare wild edible mushroom termitomyces robustus and belongs to the technical field of macrofungi culture. The preparation method is characterized in that sporocarp which is not opened at the cap is used as a material, and solid cultivated strain is prepared by using ways such as mycelium induction, mycelium amplication culture and rapid propagation. According to the invention, termitomyces robustus mycelia is special extremely slow in growing and difficult to propagate on a solid culture medium, and is difficult to grow on a cultivation medium, batch strains are difficultly produced, and the condition is one of factors restricting or influencing artificial culture. According to the invention, the termitomyces robustus mainly producedin Yunnan is taken for an example, and a creative break is generated for the solid culture technology of the mycelia, so that the mycelia can rapidly grow and propagate, culture period is greatly shortened, and bagged cultivated strain is produced. In the preparation method provided by the invention, mother strain, amplification and cultivation culture mediums are used, and are simple and available in components, low in cost and convenient for popularization, such as cultivated strain culture medium mainly makes use of primitive environment soil and wheat bran, which provides conditions for artificial domestication and cultivation.

Description

technical field [0001] The invention relates to a preparation method of a rare wild edible fungus Termitomyces robustus (Beeli.) Heim, which belongs to the field of biotechnology, and specifically belongs to the category of indoor artificial cultivation of large fungal mycelium. Background technique [0002] Termitomyces belongs to Basidiomycotina, Hymenomycetes, Agaricales, and Tricholomataceae. Because it has a symbiotic relationship with termites, it is also known as the genus Antnest . Jizong, also known as Jizong (brown), umbrella mushroom, chicken shredded mushroom, termite mushroom, etc., is a famous and precious specialty wild edible fungus in Yunnan Province, and it is also a famous delicious edible fungus in the world. It is rich in nutrition, The meat is tender, delicious, and has obvious pharmacological effects, and is deeply loved and favored by consumers at home and abroad. Domestic chicken fir fungus is mainly distributed in Yunnan. It is reported that there...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00C05G3/00
Inventor 李宗菊张曦予张丽萍杨振升陶乔双李文莲
Owner YUNNAN UNIV
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