Hypsizigus marmoreus spawn quality detection method

A detection method, the technology of Japonica, which is applied in the fields of botanical equipment and methods, color/spectral characteristic measurement, and material analysis by observing the influence of chemical indicators, etc., which can solve the problems of poor accuracy, economical operability, etc. problem, to achieve the effect of simple and efficient evaluation

Inactive Publication Date: 2012-04-11
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

[0007] The object of the present invention is to provide a kind of detection method of the pros and cons of the quality of Japonica fungus bacterial classification of improvement, promptly systematic, simple and effective monitoring method of the quality of Japonysia japonica bacterial classification, it can overcome existing method accuracy, Some shortcomings such as poor economy and operability

Method used

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  • Hypsizigus marmoreus spawn quality detection method
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  • Hypsizigus marmoreus spawn quality detection method

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Embodiment

[0017] The present invention first establishes reference parameters for the strains to be evaluated, and measures the color reaction and absorbance value of the culture solution; taking the above parameters as a benchmark, the strains to be evaluated are integrated and quantitatively evaluated.

[0018] The embodiment of the present invention is carried out according to the following four steps.

[0019] 1. Strains to be evaluated:

[0020] The strains to be evaluated are shown in Table 1, and based on this, an integrated quantitative evaluation standard system for the quality of Amanita species was constructed.

[0021] Table 1 The strains of Shijiji mushrooms tested

[0022]

[0023] 2. Nutrient source and culture medium for evaluation (g / L)

[0024] Potato 200.0 (soak juice to remove slag), lactose 20.0, NH 4 NO 3 2.0, MgSO 4 ·7H 2 O0.5, KH 2 PO 4 1.5, bromothymol blue (BTB) 0.06, pH 7.0. Take the original mycelium block from the PDA culture medium and put it ...

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Abstract

The invention discloses a hypsizigus marmoreus spawn quality detection method, which can solve the problems which are difficult to overcome by the present edible fungi spawn quality evaluation method such as systematicness, simplicity, convenience, high efficiency and the like. Due to the adoption of the detection method, the correlation of the evaluation result to yield traits such as yield of a single bottle, valid sporocarp quantity, weight of single sporocarp and the like can reach a remarkable level, the correlation of the evaluation result to mycelium growth speed in an identical test tube cultivation culture medium can reach an ordinary remarkable level, so spawn quality evaluation indexes can be separately evaluated through the method. At the same time, the evaluation indexes are adopted as a constitutional part to carry out the integrated quantitative evaluation on the hypsizigus marmoreus spawn quality, so the systematicness, the simplicity, the convenience and the high efficiency in evaluating the hypsizigus marmoreus spawn can be realized. In addition, the evaluation method provides a powerful reference for the integrated quantitative evaluation of other edible fungi spawn quality.

Description

Technical field: [0001] The present invention relates to the technical field of edible fungi, specifically a systematic, convenient and efficient method for detecting the quality of Japonica species, including required raw materials, formula, sterilization, inoculation, cultivation, spectrum A series of processes of analysis and integration of quantitative evaluation. Background technique: [0002] The quality of strains is the foundation of the development of edible fungi industry. However, with the continuous development of my country's edible fungi industry, the problem of strain quality has become increasingly prominent, and has gradually become one of the bottlenecks hindering the development of my country's edible fungus industry. In recent years, my country's edible fungus strain quality supervision system and quality control system have been continuously improved, providing an increasingly powerful institutional guarantee for standardizing the breeding, production, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04G01N21/25G01N21/78
Inventor 冯志勇刘洋陈辉陈明杰汪虹
Owner SHANGHAI ACAD OF AGRI SCI
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