New strain of Ganoderma helvetii and artificial cultivation method thereof
A technology of artificial cultivation of Hershey's Ganoderma lucidum, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve problems such as lack of development, and achieve the effect of good fruiting body properties and high polysaccharide content
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[0027] In October 2015, members of our research group collected a specimen of Ganoderma lucidum in Shiwanda Mountain, Guangxi. Wild fruiting bodies of Ganoderma lucidum such as figure 1 shown. The pure culture of PDA was obtained by the tissue separation method by scientific researchers, and the number is HMGIM-D151177. The preparation and identification process of PDA pure culture is as follows:
[0028] 1. Strain isolation:
[0029] Put the comprehensive PDA medium (potato 20%+glucose 2%+agar 2%+potassium dihydrogen phosphate 0.3%+magnesium sulfate 0.15%+vitamin B1 trace) into test tubes, and sterilize at 0.11MPa atmospheric pressure and 121℃ high temperature and high pressure After 30 minutes, take it out to cool and place it on a slope. After the collected wild fruiting bodies are wiped with 75% alcohol on the surface under aseptic conditions, they are torn open, and the internal bacterial flesh tissue of 0.2-0.5mm×0.2-0.5mm is inserted in an aseptic operation. Place ...
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