New strain of Ganoderma helvetii and artificial cultivation method thereof

A technology of artificial cultivation of Hershey's Ganoderma lucidum, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve problems such as lack of development, and achieve the effect of good fruiting body properties and high polysaccharide content

Active Publication Date: 2021-11-23
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Problems solved by technology

The current research is limited to the classification of this new species of Ganoderma lucidum, mainly describing the macrosc

Method used

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  • New strain of Ganoderma helvetii and artificial cultivation method thereof
  • New strain of Ganoderma helvetii and artificial cultivation method thereof
  • New strain of Ganoderma helvetii and artificial cultivation method thereof

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Embodiment 1

[0027] In October 2015, members of our research group collected a specimen of Ganoderma lucidum in Shiwanda Mountain, Guangxi. Wild fruiting bodies of Ganoderma lucidum such as figure 1 shown. The pure culture of PDA was obtained by the tissue separation method by scientific researchers, and the number is HMGIM-D151177. The preparation and identification process of PDA pure culture is as follows:

[0028] 1. Strain isolation:

[0029] Put the comprehensive PDA medium (potato 20%+glucose 2%+agar 2%+potassium dihydrogen phosphate 0.3%+magnesium sulfate 0.15%+vitamin B1 trace) into test tubes, and sterilize at 0.11MPa atmospheric pressure and 121℃ high temperature and high pressure After 30 minutes, take it out to cool and place it on a slope. After the collected wild fruiting bodies are wiped with 75% alcohol on the surface under aseptic conditions, they are torn open, and the internal bacterial flesh tissue of 0.2-0.5mm×0.2-0.5mm is inserted in an aseptic operation. Place ...

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Abstract

The invention belongs to the technical field of cultivation of valuable and rare medicinal fungi, and discloses a new strain of Ganoderma helvetianum and an artificial cultivation method thereof. The new strain of the Ganoderma hoehnelianum (HMGIM-D151177) is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on May 12, 2021, and the preservation number of the new strain of the Ganoderma hoehnelianum is GDMCC No: 61660. The artificial cultivation method of the new strain of the Ganoderma helvetianum comprises the steps of production mother strain manufacturing, production strain manufacturing, artificial domestication cultivation and cultivation management. According to the new strain of the Ganoderma helvetii and the artificial cultivation method thereof, the Ganoderma helvetianum is a new variety which is not researched yet, through separation and artificial domestication experiments of wild strains, the characteristics of artificial cultivation, good sporocarp character, high polysaccharide content and the like can be achieved, and the Ganoderma helvetianum is a strain with development prospects.

Description

technical field [0001] The invention belongs to the technical field of cultivation of rare medicinal fungi, and in particular relates to a new strain of Ganoderma lucidum and an artificial cultivation method thereof. Background technique [0002] At present, the edible and medicinal fungi industry is developing rapidly. According to the statistics of the China Edible Fungi Association, my country's edible fungi has developed into the fifth largest crop after grain, vegetables, fruit, and sugar. Today, the edible and medicinal fungi industry is booming. More and more rare varieties of edible and medicinal fungi have gradually entered people's field of vision, and many original rare varieties have gradually been domesticated, such as bamboo fungus, tea mushroom, lily mushroom, morel and so on. However, there are also a large number of wild edible and medicinal fungi that have not been studied because they have not been recognized by humans. According to research, there are cur...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G18/00A01G18/20A01G18/40C12R1/645
CPCC12N1/14A01G18/00A01G18/20A01G18/40
Inventor 胡惠萍刘远超蔡曼君梁晓薇陈晓光吴晓贤卓丽君李向敏王傲杜娜谢意珍吴清平肖春陈地灵陈少丹雍天乔高雄
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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