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Hericium erinaceus mother strain culture medium and making method thereof

A technology of mother seed culture medium and Hericium erinaceus, applied in fertilizer mixture, fertilization device, application, etc., can solve the problems of uneven distribution, nutrient consumption, and the decline of strain activity, so as to enhance growth vigor and reduce nutrient loss. , the effect of improving quality

Inactive Publication Date: 2013-09-18
邬金飞
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

After the mother species is inserted into the original species of Hericium erinaceus, the mycelium grows sparsely, slenderly, and the distribution is uneven. Often, the culture material begins to form a primordium before the mycelium is half full, resulting in a large consumption of nutrients and a decline in the vitality of the bacteria.

Method used

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Embodiment Construction

[0025] A kind of Hericium erinaceus stock medium formula in the present embodiment: 200g of potato, 20g of glucose, 3g of potassium dihydrogen phosphate, 20g of agar, 1000ml of tap water, add the total mass of medium (finished product) 0.2mg / kg triacontanol .

[0026] Production Method:

[0027] ①Wash the potatoes, peel them, dig out the bud eyes, weigh 200g on a balance, and cut them into small pieces of 1cm-2cm;

[0028] ② Pour the cut potatoes into an aluminum pot, add 1000ml of water, boil and then cook for 20 minutes, so that the potatoes are crisp but not rotten;

[0029] ③Filter with 4 layers of gauze, take the filtrate, add 20g of agar, heat until the agar is completely dissolved, and then filter with 4 layers of gauze;

[0030] ④ Add water to the filtrate to 1000mL, add 20g of glucose, 3g of potassium dihydrogen phosphate, heat to dissolve, and stir evenly;

[0031] ⑤ Dispense into test tubes of Φ20mm×200mm while hot, the volume of each test tube is 5ml, and then s...

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Abstract

The invention relates to a hericium erinaceus mother strain culture medium. The hericium erinaceus mother strain culture medium is characterized in that melissyl alcohol with certain concentration is added to a PDA (Potato Dextrose Agar) enriched medium. The invention also relates to a making method of the hericium erinaceus mother strain culture medium. Compared with an existing PDA culture medium, the hericium erinaceus mother strain culture medium disclosed by the invention has the advantages that the growth vitality of hericium erinaceus mycelium is enhanced because the melissyl alcohol stimulates the energy metabolism of mycelium, so that the fungi culture time of a mother strain is shortened, the fungi culture time of an original strain is also shortened by making a hericium erinaceus original strain by using the mother strain, therefore, the differentiation phenomenon of a sporocarp during a strain germination period is avoided, the nutrient loss is reduced and the strain quality is improved.

Description

technical field [0001] The content of the present invention includes the medium formula of Hericium erinaceus mother species and the preparation method of the medium, belonging to the technical field of edible fungus seed production. Background technique [0002] Hericium erinaceus, the fruiting body is round and thick, covered with needle-like thorns, shaped like a monkey's head, hence the name. Hericium erinaceus is known as a mountain delicacy, and it is a high-grade edible and medicinal fungus. It can treat diseases such as indigestion, gastric ulcer and neurasthenia. In recent years, domestic and foreign medicine has proved that Hericium erinaceus has a good auxiliary effect on malignant tumors of the digestive system such as gastric cancer and esophageal cancer. The good medicine made from it includes monkey mushroom slices and so on. [0003] The growth and development of Hericium erinaceus has strict requirements on nutritional conditions. Under different nutrition...

Claims

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Application Information

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IPC IPC(8): C05G1/00
Inventor 邬金飞江伟
Owner 邬金飞
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