82 results about "Wild type strain" patented technology

The invention provides a clostridium endophyllum engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biology, compared with a wild type strain, the clostridium endophyllum engineering bacterium can only produce acetic acid in a low-pH environment under the condition of taking CO2 and H2 as raw materials, and does not generate by-products such as ethanol, lactic acid, 2, 3-butanediol and the like. Besides, the fermentation liquor of the clostridium endophyllum engineering bacteria can be directly used as an acidic substrate to adjust the pH value of downstream yeast fermentation liquor, meanwhile, automatic addition of a carbon source is achieved, zero addition of inorganic salt in the cascade conversion process from CO2 and H2 to grease or yeast protein is achieved, and the economical efficiency and environmental friendliness of the whole process are improved.

The invention relates to application and a method of a biological material for regulating and controlling MYB4 gene overexpression in the aspect of improving the yield of pleurotus edible fungi. The biomaterial exerts an effect of increasing the yield of a Pleurotus edible fungus by overexpressing MYB4 in the fungus. Experimental results show that the expression quantity of the MYB4 gene in the overexpression strain is remarkably higher than that of a wild strain, and the yield of the strain with the overexpression MYB4 gene during fruiting is remarkably improved compared with that of the wild strain.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an OsNAC4 gene in regulation and control of rice salt tolerance. It is found for the first time that mutation of the OsNAC4 gene can improve the salt tolerance of rice seeds in the seedling stage. Under salt stress, the seedling survival rate of the mutant OsNAC4 strain is obviously higher than that of a wild type. Under normal treatment, the proline content, the hydrogen peroxide content and the malondialdehyde content of the wild type strain and the Osnac4 mutant strain have no significant difference, but after salt stress, the proline content of the Osnac4 mutant strain is significantly higher than that of the wild type strain, and the hydrogen peroxide content and the malondialdehyde content are both lower than those of the wild type strain. Therefore, the OsNAC4 gene can be utilized to cultivate an improved rice material suitable for saline-alkali soil planting through a biotechnology, a new thought is provided for cultivating salt-tolerant rice varieties, and a theoretical basis is also provided for increasing the yield of other crops by utilizing a heterologous gene expression technology.

The invention discloses recombinant xanthomonas campestris with xanthan gum hydrolase displayed on the surface and a construction method and application of the recombinant xanthomonas campestris. The recombinant xanthomonas campestris for surface display of xanthan hydrolase is constructed by introducing a recombinant vector obtained by fusion expression of an ice crystal nuclein (INP) gene and a xanthan hydrolase gene into xanthomonas campestris. The gel yield of recombinant xanthomonas campestris fermentation is twice that of a wild strain and reaches 6.7 g / L. The strain can efficiently hydrolyze xanthan gum with high molecular weight (such as more than 100 wDa), the traditional production process which is relatively complicated is simplified, and a new method is provided for large-scale production of the xanthan gum with low molecular weight.

The application discloses a glycerol dehydrogenase mutant and application thereof, and the glycerol dehydrogenase is derived from Klebsiella pneumoniae and is encoded by gene dhaD. The application improves the molecular structure of the glycerol dehydrogenase by rational design and a site-directed mutagenesis technology to obtain a mutant enzyme F245Q / GDH, and the mutant enzyme is back-supplemented into Klebsiella pneumoniae which is deficient in glycerol dehydrogenase. Compared with a wild-type strain, the yield of a main product 1,3-propanediol of the back-supplemented mutant enzyme F245Q / GDH strain is improved, and the yield of a by-product 2,3-butanediol is significantly reduced.

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, the mutant is obtained by mutation of an amino acid sequence of amylosucrase derived from coprophilobacterium mediterranei, and the amino acid sequence of the mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. Compared with a wild strain, the catalytic activity, the temperature stability and the substrate tolerance of the amylosucrase mutant disclosed by the invention are remarkably improved; the recombinant bacteria constructed by using the recombinant bacteria can be used as a whole-cell catalyst to efficiently catalyze a substrate to synthesize alpha-arbutin in a reaction system containing hydroquinone. Therefore, the amylosucrase mutant disclosed by the invention has a good application prospect.

The invention provides a pseudomonas aeruginosa engineering bacterium which is based on a PAO1 wild type strain, and two of the following genes, namely cyaA and cyaB, are knocked out from a genome at the same time. According to the pseudomonas aeruginosa engineering bacterium, the cyaA gene and the cyaB gene are knocked out at the same time, and the yield of rhamnolipid is remarkably increased on the premise that existing PAO1 fermentation process parameters and processes are not changed. The yield cannot be increased when the cyaA or the cyaB is singly knocked out, and the synergistic effect of double-gene knockout is proved.

The present invention discloses an L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid. In the present invention, through combined multi-site amino acid mutation, a technical effect of significantly improved enzyme activity of the mutant AkAGATT225Q / A258P / L278K than that of the wild-type strain is achieved, providing an application value for large-scale production of guanidinoacetic acid in industry. When the L-arginine-glycine amidinotransferase mutant constructed in the present invention is used in the production of guanidinoacetic acid, by optimizing the conversion conditions, the yield of guanidinoacetic acid is up to 21.4 g / L and the conversion rate is 90.4%, after 24 hrs of reaction in a 1 L reaction system. Compared with the production of guanidinoacetic acid with the raw enzyme, the yield is increased by 49.6%.

Genetically transformed fungal strains for preparing a leather-like bioproduct and methods for obtaining said bioproduct, wherein the transformed strain has an increased carbohydrate hydrolytic activity as compared to the parent wild type strain and overexpressing genes such as swi6B, glsnf 1, gcn4, and / or nmnat.

The invention discloses a genetically engineered bacterium for producing heparinogen and a construction method and application thereof, the genetically engineered bacterium takes Escherichia coli Nissle 1917 as a chassis bacterium, and the construction method comprises the following steps: (1) knocking out endonuclease I coding gene endA in a chassis bacterium genome; (2) knocking out a 6-phosphofructokinase I coding gene pfkA in a chassis bacterium genome; (3) carrying out overexpression on a 6-phosphoglucosamine synthetase encoding gene glmS and / or a UDP-glucose-6-dehydrogenase encoding gene kfiD from a chassis bacterium genome; (4) removing the feedback inhibition of the 6-phosphate glucosamine on the GlmS so as to promote the synthesis of UDP-N-acetylglucosamine; and (5) knocking out the 6-phosphate glucosamine deaminase coding gene nagB in the chassis bacterium genome. The engineering strain obtained by the invention can more efficiently synthesize the proheparin, the shake flask titer reaches 397.9 mg / L, which is increased by about 3 times compared with a wild strain, and the engineering strain has certain industrial application value.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The invention discloses an application of a regulatory gene FgCTF1 beta 1 for synthesizing deoxynivalenol from fusarium graminearum. The regulatory gene FgCTF1 beta 1 is used for synthesizing deoxynivalenol from fusarium graminearum. According to the invention, a gene engineering strain delta FgCTF1 beta 1 is obtained by knocking out an FgCTF1 beta 1 gene in a fusarium graminearum genome. Compared with a wild strain PH-1, the genetic engineering strain delta FgCTF1 beta 1 has no significant change in the aspects of mycelial growth, conidium generation, pathogenicity to wheat coleoptiles and the like, but the biosynthesis amount of deoxynivalenol is significantly reduced. The invention also provides application of the FgCTF1 beta 1 gene as an inhibition target or a knockout target in inhibition of synthesis of DON from fusarium graminearum, and provides a new strategy and technical approach for prevention and control of pollution of deoxynivalenol in agricultural products.

The invention discloses an acetate-resistant domesticated algal strain HpAc50 and an application thereof. The algal strain is preserved in the China Center for Type Culture Collection on January 12, 2026, and the preservation number is CCTCC M 202690. The haematococcus pluvialis strain is obtained by carrying out acetate-resistant domestication on haematococcus pluvialis and can be cultured under the condition that the acetic acid concentration is 0-10g / L, and the yield of astaxanthin is effectively increased. The invention specifically provides three application conditions: illumination of 0-5000lux and a full-nutrient culture medium; illumination is performed at 0-5000 lux, a nitrogen-phosphorus-deficient culture medium is adopted, and an exogenous stimulant is added According to the present invention, with the application of the strain, the cell density, the biomass and the astaxanthin accumulation can be significantly improved under the conditions of illumination of 5000-15000 lux and nitrogen and phosphorus deficiency culture medium, such that the strain has strong acetate tolerance and strong production performance compared with the wild type strain, and is suitable for the large-scale astaxanthin production.

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, and belongs to the technical field of genetic engineering. An amylosucrase mutant is obtained through an error-prone PCR technology, the mutant is obtained by mutation on an amylosucrase amino acid sequence derived from coprophilobacterium mediterranei, and the amino acid sequence of the amylosucrase mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. Compared with a wild strain, the amylosucrase mutant disclosed by the invention has higher catalytic activity, temperature stability and substrate tolerance; when the catalyst is added into a hydroquinone-containing reaction system, alpha-arbutin can be efficiently catalyzed and synthesized. Therefore, the amylosucrase mutant disclosed by the invention is suitable for industrial production and has a good application prospect.

The application belongs to the technical field of bioengineering, and discloses a gene engineering bacterium of acetobacter pasteurii with high temperature resistance, a construction method and application thereof. Acetobacter pasteurianus The gene engineering bacterium is constructed by using the acetobacter pasteurii (ATCC 10137) as a starting strain and knocking out the transposase gene in the genome of the acetobacter pasteurii (ATCC 10137) by a homologous recombination method. TnsR The gene engineering bacterium with high temperature resistance is obtained. TnsR The deletion of the gene is beneficial to maintaining the stability of the genome, so that the strain can maintain better growth and metabolic capacity under high-temperature pressure. Experimental results show that the biomass, survival rate and acetic acid yield of the gene engineering strain under the condition of 40 DEG C high temperature are significantly better than those of the wild-type strain. When the gene engineering strain is applied to traditional solid-state food vinegar brewing, the yield of organic acids such as acetic acid is significantly improved, the content of ester flavor substances is greatly improved, and the flavor quality of food vinegar is effectively improved. The application provides an effective technical means for solving the problem that high temperature in summer leads to abnormal fermentation and yield reduction of food vinegar.

The invention relates to the cross technical field of molecular biology and parasitology, and particularly discloses a giardia living body marking method based on fluorescent protein UnaG, which comprises the following steps: firstly, optimizing and synthesizing a UnaG gene sequence according to the codon use preference of giardia; then, constructing a marker plasmid pGT-UnaG-neo on a double expression framework containing an alpha2-tubulin promoter and a gdh promoter, and introducing the plasmid into the giardia giardia trophozoite through electroporation. And carrying out resistance screening in a culture medium containing geneticin to obtain an insect strain capable of stably integrating and expressing UnaG. Through bilirubin induction, efficient maturation and green fluorescence emission of the UnaG protein in giardia cytoplasm are realized. The stably transferred strain obtained by the method has no significant difference from a wild strain in the aspects of growth curve and survival rate, and UnaG and bilirubin have no obvious toxic effect. The limitation that traditional fluorescent protein is inactivated under the anaerobic condition is broken through, and a reliable tool is provided for dynamic imaging, functional research and drug screening of giardia and other anaerobic protozoa.

This invention discloses a desmethylmenoprenone methyltransferase mutant, its construction method, and its applications, belonging to the field of biotechnology. Desmethylmenoprenone methyltransferase is a key enzyme in the MK-7 biosynthetic pathway, responsible for catalyzing the methylation reaction of desmethylmenoprenone to generate a series of methylnaphthoquinone (MK) products, ultimately forming MK-7. This invention, through metabolic network regulation and rational design, successfully obtained 19 single-point mutants, among which 5 mutants showed significantly higher catalytic efficiency than the wild-type enzyme. Experimental results show that the recombinant engineered bacteria constructed based on these mutants exhibit significant advantages in the whole-cell catalytic production of vitamin K2 (MK-7), with MK-7 yield increased by more than 20% compared to the wild-type strain. The excellent catalytic performance of the menG mutant provided by this invention provides strong technical support for the industrial production of vitamin K2, and has broad application prospects and market value.

The application relates to application of a biological material related to an SDR32 gene in regulating yield and / or high-temperature tolerance of Pleurotus edulis. The biological material plays a role in improving the yield and / or high-temperature tolerance of the fungus by overexpressing SDR32 in the edible fungus of the genus Pleurotus. Experimental results show that the expression amount of the SDR32 gene in the overexpression strain is significantly higher than that in the wild type strain, the yield of the strain overexpressing the SDR32 gene during fruiting is significantly improved compared with the wild type strain, and the high-temperature tolerance of the strain overexpressing the SDR32 gene is significantly enhanced compared with the wild type strain.

The invention discloses paenibacillus polymyxa as well as a construction method and application thereof. Antagonistic bacteria are screened from soil suffering from wheat stem rot, a paenibacillus mucilaginosus strain with broad-spectrum bacteriostasis and prevention and treatment effects is screened out through enrichment culture, the paenibacillus mucilaginosus strain is named as Paenibacillus polymyxa ZDW-24-15 and preserved in the China Center for Type Culture Collection on March 10, 2025, the address is No. 299 on eight road in Wuchang District, Wuhan City, China, and the preservation number is CCTCC NO: 2025412. According to the present invention, the recombinant ZDW-24-15-[delta] abrB bacterial strain is further obtained by carrying out gene modification on the ZDW-24-15 bacterial strain, and the recombinant ZDW-24-15-[delta] abrB bacterial strain shows the more excellent antibacterial property than the wild type ZDW-24-15 bacterial strain.

The invention discloses a strain of spiropolyspora pink MLY32, a microbial inoculum as well as a preparation method and application of the microbial inoculum. The MLY32 strain is obtained by multi-generation co-culture, domestication and breeding of a wild-type spiropolyspora roseus strain CR032 separated from rhizosphere soil of Guizhou Xinyi healthy tobacco plants and a wild-type phytophthora parasitica strain PpN03, can be attached to phytophthora parasitica hyphae through parasitism, and shows unique explosive growth, and the MLY32 strain can be applied to the field of tobacco phytophthora parasitica hyphae. After the MLY32 strain is parasitized, the proliferation speed of the parasitized tobacco phytophthora parasitica wild type strain PpN03 is greatly slowed down, so that the MLY32 strain has a strong bacteriolytic inhibition effect on the tobacco phytophthora parasitica, and the MLY32 strain is co-cultured with the non-pathogenic tobacco phytophthora parasitica MLY61 to obtain a fungicide spiropolyspora 3261 which still has strong parasitism and no pathogenic risk on the tobacco phytophthora parasitica wild type strain PpN03. The invention has wide application potential in the field of agricultural biological control fungicide.

The invention discloses a gene FgPIP1 for regulating and controlling polar growth and pathogenicity of the top end of fusarium graminearum and application of the gene FgPIP1, and belongs to the field of plant disease prevention and control. According to the invention, a novel pathogenic gene FgPIP1 with specific existence of filamentous ascomycetes is found in plant pathogenic fungi fusarium graminearum, and the nucleotide sequence of the pathogenic gene FgPIP1 is shown as SEQ ID NO. 1. Compared with a wild strain PH-1, the FgPIP1 gene knockout mutant has the advantages that the diameter of a vegetative growth colony of the mutant is reduced, the number of apical branches of hyphae is increased, and the pathogenicity to wheat ears is reduced, which indicates that the FgPIP1 gene plays an important role in regulating and controlling the pathogenic process of fusarium graminearum. The invention provides a molecular basis for developing a novel bactericide by utilizing the FgPIP1 gene and taking inhibition of the top growth of filamentous fungus hyphae as a target spot.

This invention relates to the field of microbiology, specifically to a high-ApxI toxin-producing APP strain, its construction method, and its applications. This invention provides a strain of *Actinobacillus pleuropneumoniae*, with the accession number CGMCC No. 25495. The *Actinobacillus pleuropneumoniae* exhibits an ApxI toxin yield as high as 2.367 ± 0.25 mg / L, more than three times that of the wild-type strain. This invention provides a new approach and method for constructing high-ApxI toxin-producing strains, offering a new strain resource for the production of *Actinobacillus pleuropneumoniae* subunit vaccines.

The invention discloses candida maltose and application of the candida maltose in production of feed protein. The selected candida maltose is a wild strain separated from wood rotten substances, and has the capacity of rapidly metabolizing monosaccharide / disaccharide such as glucose, xylose and cellobiose and accumulating microbial protein. The saccharomycetes can be co-cultured and fermented with trichoderma reesei and aspergillus niger, and lignocellulose agricultural wastes such as corncobs and the like are used as raw materials to produce feed protein through one-step fermentation. The invention provides an excellent strain and an effective method which can be used for producing feed protein, and has wide application value in the field of microbial protein.

We have shown that cprA expression leads to the production of outer membrane vesicles (OMVs) that block autophagic flux and have a greater capacity to activate the non-canonical inflammasome pathway. In a murine model of sepsis, a P. aeruginosa strain deleted for cprA was less virulent than the wild-type strain. These results demonstrate the important role of CprA in the pathogenicity of P. aeruginosa. It is worth noting that CprA is also a functional ortholog of HlyF, which is encoded by virulence plasmids of Escherichia coli. We have shown that other cryptic SDRs encoded by mammalian and plant pathogens, such as Yersinia pestis and Ralstonia solanacearum are functional orthologs of CprA and HlyF. These SDRs also induce the production of OMVs which block autophagic flux. This study uncovers a new family of virulence determinants in Gram-negative bacteria, offering potential for innovative therapeutic interventions and deeper insights into bacterial pathogenesis. The present invention relates to a method for producing fatty alcohols in a medium comprising HlyF protein, or an ortholog of HlyF protein, and fatty acyl-CoA.

The invention discloses a genetically engineered bacterium for producing D-pantothenic acid as well as a construction method and application of the genetically engineered bacterium, wild type C. glutamicum ATCC 13032 is taken as a chassis bacterium, an ilvE gene, an avtA gene, an ilvA gene and a pqo gene in an original strain genome are knocked out, ilvBNC from different sources and mutants thereof are screened, an optimized mutant ilvBNC-VWBGTG / ATG is determined, the gene cluster and promoters Ptuf and Pefu are connected and integrated to a strain DPAe-2, and the D-pantothenic acid is produced. An engineering strain DPAg-11 capable of efficiently accumulating KIV is obtained, then a promoter of an aceE gene is replaced by PdapA A16, KIV accumulated by the obtained engineering strain DPAg-15 reaches 3.99 g / L, panB, panC, panE or ilvC from different sources are screened, it is determined that BspanBC + EcpanE has the best effect, BspanBC + EcpanE is constructed on plasmids and then converted into various engineering strains, a promoter of an EcpanE gene is replaced by PdapB, and KIV accumulated by the engineering strain DPAg-11 and PdapA A16 reaches 3.99 g / L. The method has the advantages that the method is simple and easy to implement, a substrate channel can be built between dihydroxy acid dehydratase (DHAD) and KPR by polypeptide pair RIAD-RIDD, D-PA produced by the finally obtained strain reaches 3.21 g / L and is increased to 97.0 times that of DPA produced by a wild strain, and production of D-PA can be effectively promoted by the strategy of the built substrate channel.

The present invention relates to a method for high production of heparic acid using a recombinant E. coli strain and to the heparic acid produced thereby. The E. coli strain according to the present invention has the advantage of efficiently increasing the flux of the entire heparic acid biosynthetic pathway through the optimization of the dual plasmid system, promoter combination, and culture medium, thereby increasing the heparic acid production compared to the wild-type strain and enabling the securing of high-purity heparic acid even through a simple purification process.

The invention discloses a saccharomyces bisporus evolved strain F-9 with trans-oleic acid high metabolism capability, and the preservation number of the saccharomyces bisporus evolved strain F-9 in the general microbiological center of the China Committee for Culture Collection of Microorganisms is CGMCC No.39075. The saccharomyces bisporus evolved strain F-9 is a saccharomyces bisporus evolved strain F-9 with trans-oleic acid high metabolism capability, and the preservation number of the saccharomyces bisporus evolved strain F-9 is CGMCC No.39075. The saccharomyces bisporus evolutionary strain F-9 is obtained by taking a wild strain F3404 as an original strain and carrying out induction culture on the original strain at 25-35 DEG C under a sealed condition by adopting a concentration gradient incremental method, the metabolic rate of the evolutionary strain F-9 to trans-oleic acid is 33.78 + / -1.51%, and the evolutionary strain F-9 is excellent in genetic stability after continuous passage for 20 generations; the invention provides a new way for degrading or converting trans-oleic acid.

The invention discloses a fusarium venanii strain with high substrate conversion rate and low RNA (Ribonucleic Acid) content and application of the fusarium venanii strain, and belongs to the fields of genetic engineering technology and food biotechnology. Compared with a wild strain, the fusarium venanii Fcdc has the advantages that the conversion rate of a gene Cdc42 on a genome from a substrate to thallus is increased to 67.06%, the conversion rate from the substrate to protein is increased to 26.01%, the sugar consumption for producing mycelia with the same dry weight is reduced by 60%-70%, the sugar consumption for producing mycelia protein with the same dry weight is reduced by 60%-70% compared with the wild strain, and the RNA content of the mycelia is reduced by more than 90% and is as low as 0.32% compared with the wild strain; the method has the advantages that the method is simple and convenient to operate, a heat treatment and nucleic acid removal step is not needed, the production efficiency and the safety quality of the fusarium venanii hypha protein are obviously improved, the production cost is reduced, and the fusarium venanii can be used for further developing genetic engineering modification work and cultivating fusarium venanii strains with high protein content and low RNA content.

This invention relates to the field of agricultural biotechnology, specifically to regulatory genes. Mt_2312847 Application in increasing cellulase expression levels in *Thermophilus thermophilus*. This is achieved by interfering with or knocking out regulatory genes in wild-type *Thermophilus thermophilus* strains. Mt_2312847, Knockout strain (Mt) Delta 2312847), the cellulase concentration in the fermentation broth of the interfering strain was higher than that of the wild strain, Mt Delta The CMCase activity of strains 2312847 and Mt_2312847i was 2.2 times and 1.6 times that of the control strain, respectively.

The application relates to application and methods of a biological material for regulating overexpression of a MYB4 gene to improve yield of an edible fungus of the genus Pleurotus. The biological material plays a role in improving the yield of the edible fungus of the genus Pleurotus by overexpressing MYB4 in the edible fungus of the genus Pleurotus. Experimental results show that the expression amount of the MYB4 gene in the overexpression strain is significantly higher than that in the wild type strain, and the yield of the overexpression MYB4 gene strain during mushroom production is significantly improved compared with the wild type strain.