Novel prrs virus inducing type i interferon in susceptible cells

a virus and susceptible cell technology, applied in the field of attenuated live viruses, can solve problems such as complicated prrsv infection

Active Publication Date: 2013-01-31
BOEHRINGER LNGELHEIM VETMEDICA GMBH
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  • Abstract
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  • Application Information

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Problems solved by technology

Often, PRRSV infection is complicated by secondary bacterial infections being attributed to the immunosuppressive nature of the virus.

Method used

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  • Novel prrs virus inducing type i interferon in susceptible cells
  • Novel prrs virus inducing type i interferon in susceptible cells
  • Novel prrs virus inducing type i interferon in susceptible cells

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[0140]In the examples five viable, genetically designed PRRSV mutant strains are described which are based on infectious EU PRRSV cDNA clone LoN94-13. These strains, delta nsp1 IX-10, delta nsp1 XVII-1, delta nsp1 XVIII-12, delta nsp1 XIX-2 and delta nsp1 XX-9 (henceforth referred to as vaccine candidates), harbor genomic deletions of two, three, four, five, or six codons in their predicted nsp1 genes, respectively, resulting in deletions of two (motif P21R22), three (motif R20P21R22), four (motif G19R20P21R22), five (motif N18G19R20P21R22), or six (motif (P17N18G19R20P21R22) amino acids in their predicted nsp1β proteins, respectively (FIG. 1).

[0141]Based on sequence alignments of parental strain LoN94-13 with PRRSV US and EU reference strains VR-2332 and Lelystad virus as well as with strain GD-XH, the deletions are located in the predicted nsp1β portion of nsp 1 (FIG. 2). In more detail, the deletion site for all vaccine candidates is located in the N-terminal domain (NTD) of nsp1...

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Abstract

The present invention relates to the field of attenuated live viruses useful as vaccine or medicament for preventing or treating Porcine Reproductive and Respiratory Syndrome (PRRS) in swine, and is based on the surprising finding of a PRRS virus which is able to induce the interferon type I response of a cell infected by said virus. In one embodiment, the PRRS virus according to the invention is a PRRS virus mutant comprising, in comparison with the genome of a wild type strain, a mutation in the gene encoding the non structural protein 1 (nsp1) of said virus.

Description

SEQUENCE LISTING[0001]The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jul. 29, 2011 is named 01-2738-SEQ.txt and is 236,320 bytes in size.FIELD OF THE INVENTION[0002]The present invention belongs to the field of vaccines and medicaments for the prophylaxis and treatment of infectious diseases. In particular, it relates to attenuated live viruses useful as vaccine or medicament for preventing or treating Porcine Reproductive and Respiratory Syndrome (PRRS), a viral disease affecting swine.BACKGROUND OF THE INVENTION[0003]Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the virus family Arteriviridae and belongs, together with the Coronaviridae, to the virus order Nidovirales. PRRSV is an enveloped virus with a single-stranded, positive-sense RNA genome of about 15 kilobases comprising nine open reading frames (ORFs), n...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N7/00C12N7/04C12N15/40C12N15/85C12Q1/70A61P31/14G01N21/55G01N21/64G01N21/78G01N21/75A61K39/12A61P37/04C12N7/01C12N5/10
CPCC07K14/005C12N7/00C12N2770/10062C12N2770/10022C12N2770/10021A61P31/14A61P37/04A61K35/76A61K39/12A61K2039/552C12N2770/10034
Inventor GALLEI, ANDREAS
Owner BOEHRINGER LNGELHEIM VETMEDICA GMBH
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