Method for expanding antigen spectrum of foot-and-mouth disease vaccine strain by reverse genetic operation and preparation method of vaccine

A foot-and-mouth disease virus and vaccine technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microorganism-based methods, can solve problems such as impossible realization, time-consuming and labor-intensive vaccine strains, and unsatisfactory screening requirements. Improve the protection rate and the effect of broad antigen spectrum

Active Publication Date: 2011-01-19
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, screening new vaccine strains is time-consuming and laborious, and even the seed viruses that meet the requirements cannot be screened
What is more difficult is that there

Method used

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  • Method for expanding antigen spectrum of foot-and-mouth disease vaccine strain by reverse genetic operation and preparation method of vaccine
  • Method for expanding antigen spectrum of foot-and-mouth disease vaccine strain by reverse genetic operation and preparation method of vaccine
  • Method for expanding antigen spectrum of foot-and-mouth disease vaccine strain by reverse genetic operation and preparation method of vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, preparation and functional verification of mutant strains

[0059] 1. Construction of the full-length cDNA recombinant plasmid corresponding to the foot-and-mouth disease virus genome RNA

[0060] Design and synthesize the following oligonucleotide primers (synthesized by Shanghai Sunny Co., Ltd.):

[0061] Z1: ag Actagt ttgaaagggggcgctagggt

[0062] Z1-2: tggttggggggggggggggggtgaa

[0063] Z2: ttcaccccccccccccccccaaccaacca

[0064] Z2-2: agcgtggagtcgagcacagtac

[0065] Z3: ggtctaagcaggtttccacaactg

[0066] Z3-2: aactgcagtgcttcgtgctgccctttctcaatg

[0067] Z4: gctaagcttggaactccacgaaaaggtgtcga

[0068] Z4-2: tttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt: Nucleotides in italics are additional restriction sites and protective bases, and the following are nucleotides in the sequence].

[0069] T1: gaaaacgcctgaggccgccgcacactgcatt

[0070] T1-2: aatgcagtgtgcggcggcctcaggcgttttc

[0071] T2: gtgaagaagatatctg...

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Abstract

The invention relates to a method for expanding the antigen spectrum of a foot-and-mouth disease vaccine strain by reverse genetic operation and a preparation method of a vaccine. The amino acid sequence of the VP3 and VP1 structural proteins of the foot-and-mouth disease virus strain of the invention is represented by the amino acid residues from a position 304 to a position 736 in SEQ ID No.4. Experiments show that the vaccine prepared from the mutant virus strain obtained by the invention can resist porcine epidemic viruses of China O/TL/Taiwan/97 lineage, Pan-Asia O/China/99 lineage and Southeast Asia Myanmar O/GS/2010/98 lineage, has a characteristic of wide antigen spectrum, can immunize pigs and obviously improve the rate of protection against foot-and-mouth disease viruses which are of the same type and have antigenicity difference, achieves an immune effect of cross protection, and is expected to play an important role in the prevention and control of foot-and-mouth disease.

Description

technical field [0001] The invention relates to expanding the antigenic spectrum of foot-and-mouth disease vaccine strains by reverse genetic operation and a preparation method of the vaccine. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious infection of pigs, cattle, sheep and other cloven-hoofed animals caused by foot-and-mouth disease virus (FMDV). sick. Because of its strong infectivity, the World Organization for Animal Health (FAO / OIE) lists it as the first of the must-report epidemic diseases, and my country stipulates it as a first-class animal infectious disease. The outbreak and prevalence of the disease have reduced the productivity of domestic animals, stagnated the trade of live animals and animal products, and caused huge economic losses; at the same time, it has caused serious negative effects on public health and national reputation. Therefore, countries all over the world attach great importance to the prev...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N15/42C12N15/09C07K14/09A61K39/135A61P31/14C12R1/93
Inventor 刘在新李平花李冬孙普白兴文殷宏卢曾军陈应理谢宝霞包慧芳曹轶梅曹伟军付元芳
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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