High-yield engineering bacterium of nicomycin x component and its application

A technology of nikkomycin and recombinant engineering bacteria, which is applied in the fields of application, genetic engineering, bacteria, etc., and can solve the problems that the biosynthesis pathway of nikkomycin has not been clearly studied
CN1548526AInactive Publication Date: 2004-11-24INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2004-11-24
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The present invention belongs to the field of antibiotic gene engineering, and is especially the process of cloning nicomycin biological synthetic gene sanU from one strain of Streptomyces ansochromogenes 7100 CGMCC 4.321; connecting the synthetic gene sanU with promoter of melanin biosynthetic structure gene, cloning onto the polycloning site of integrative single-copy plamid, transforming wild Streptomyces ansochromogenes 7100 CGMCC 4.321 protoplast to obtain nicomycin X component of the recombinant engineering bacterium in the yield 2 times higher than wild strain. The high-yield engineering bacterium may be used in preparing antiseptic medicines.
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Description

technical field

[0001] The invention belongs to the field of antibiotic genetic engineering. Specifically, it involves cloning the nikkomycin biosynthesis gene sanU from a Streptomyces ansochromogenes CGMCC 4.321, connecting the sanU gene to the promoter (Pmel) of the melanin biosynthesis structural gene, and then cloning On the multiple cloning site of the single-copy integrated plasmid pSET152, and then transform wild-type Streptomyces chromogenes CGMCC 4.321 protoplasts, the yield of the recombinant engineered strain of nikkomycin X is 2 times higher than that of the wild-type strain about. The high-yield engineered bacteria can be used in the preparation of antibiotic drugs. Background technique

[0002] Streptomyces ansochromogenes CGMCC 4.321 is a nikkomycin-producing fungus isolated from soil in Northeast my country. Nicomycin is a class of nucleoside peptide antibiotics, because its structure is similar to the natural substrate UDP-N-acetylglucosamine of chitin sy...

Claims

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