A kind of method for improving nikkomycin production

Abstract

The invention provides a method for improving the output of nikkomycin. The invention specifically provides a fermentation medium for Streptomyces ansochromogenes and a method for preparing nikkomycin by using the medium. According to the invention, a proper amount of Tween 80 is added into the fermentation medium to further optimize the varieties and amounts of specific components of the fermentation medium and other culture conditions are controlled, so the output of nikkomycin is increased; the output of nikkomycin in the invention is as high as 42600 [mu]g / mL; and the method is simple, low in cost and suitable for industrial large-scale production.

Description

technical field

[0001] The invention relates to the field of microorganisms, and specifically provides a fermentation medium for Streptomyces chromogenes and a method for preparing nikkomycin by using the medium. Background technique

[0002] Nikkomycin is an important peptidyl nucleoside antibiotic. It has a good control effect on fungal diseases of crops, fruits and vegetables and some economic plants. It is also environmentally friendly and suitable for large-scale promotion and use in agricultural production. Summary of the traditional preparation methods, the method of mutagenesis and screening of excellent strains is usually used to increase the yield of nikkomycin, but this method has many uncertain factors, large screening work, slow breeding, and it is difficult to effectively increase the nikkomycin yield Shortcomings.

[0003] Nicomycin has a similar structure to UDP-N-acetylglucosamine, the natural substrate of chitin synthase, and competitively inhibits chitin ...