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Chitin decomposition bacterium and application thereof

A technology of chitin and decomposing bacteria, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of hidden dangers of pesticide residues in prevention and control methods, and achieve the effect of not producing drug resistance

Active Publication Date: 2019-01-01
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of hidden dangers of pesticide residues in the main control methods of the existing plant Fusarium oxysporum disease, and provide a chitin-decomposing bacterium and its application

Method used

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  • Chitin decomposition bacterium and application thereof
  • Chitin decomposition bacterium and application thereof
  • Chitin decomposition bacterium and application thereof

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specific Embodiment approach 1

[0017] Embodiment 1: In this embodiment, a strain of chitin-decomposing bacteria is Streptomyces ansochromogenes. F7, which is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and the preservation address is Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, the preservation date is February 02, 2018, and the preservation number is CGMCC No.15333.

[0018] In this embodiment, the separation and purification of chitin-decomposing bacteria strains are carried out in wild rice rhizosphere soil:

[0019] Preliminary screening: Using the plate dilution coating method, weigh 5g of the rhizosphere soil sample from Dongxiang wild rice in Jiangxi, put it in 45ml of sterile water, 120rpm, let it stand for 20min, and take the supernatant for 10-fold concentration gradient dilution. Take 10 respectively -1 、10 -2 、10 -3 80 μL of each concentration of diluent was applied to 3 chitin medium plates for each gradient, and ...

specific Embodiment approach 2

[0023] Embodiment 2: The application of a chitin-decomposing bacterium in this embodiment refers to the application in the prevention and control of plant diseases.

[0024] This embodiment provides a strain of Streptomyces chromogenes F7, which has chitinase activity and has an inhibitory effect on Fusarium oxysporum, obtained by screening in the rhizosphere soil of wild rice. The preservation number is CGMCC NO.15333, which is In the rhizosphere soil of wild rice in different places in Dongxiang, Jiangxi Province, the strains with strong ability to decompose chitin were obtained through primary selection, and then the strains with good antagonistic effect on Fusarium oxysporum were screened repeatedly. A large number of experiments proved that the strain F7 Not only can it decompose chitin, an important component of the cell wall of Fusarium oxysporum, but the fermentation broth of strain F7 can inhibit Fusarium oxysporum by 78%, which shows that the strain F7 has a good effe...

specific Embodiment approach 3

[0025] Embodiment 3: The difference between this embodiment and Embodiment 2 is that chitin decomposing bacteria are used in the prevention and treatment of Fusarium oxysporum. Others are the same as in the second embodiment.

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Abstract

The invention provides a chitin decomposition bacterium and application thereof. The invention relates to a chitin decomposition bacterium and the application thereof, aiming at solving the problem inthe prior art that pesticide residue hidden dangers exist in existing prevention and control of fusarium oxysporum diseases. The chitin decomposition bacterium provided by the invention is streptomyces ansochromogenes F7 and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is February 02, 2018 and the preservation number is CGMCC No. 15333. The application of the chitin decomposition bacterium provided by the invention refers to application to prevention and control of plant diseases. The strain F7 has a very good antagonistic action on the fusarium oxysporum diseases; and the inhibition effect of an F7 fermentation solution on the fusarium oxysporum diseases reaches 78 percent. The chitin decomposition bacterium provided by the invention is applied to the technical field of environment microorganisms.

Description

technical field [0001] The invention relates to a strain of chitin-decomposing bacteria and its application. Background technique [0002] Fusarium is one of the most important pathogenic fungi of crops. It is the most common parasitic pathogen and seriously affects the growth of cereal plants and food production. Among them, Fusarium oxysporum (F.oxysporum) is a typical example, which can cause more than 100 kinds of plant wilts such as melons, solanaceae, bananas, cotton, legumes and flowers. It is of great significance to develop new and efficient biocontrol agents that can ensure food safety and environmental safety. The main prevention and control method of the existing plant Fusarium oxysporum disease is still based on chemical pesticides and there are hidden dangers of pesticide residues. Contents of the invention [0003] The purpose of the present invention is to solve the problem of hidden dangers of pesticide residues in the main control methods of the existin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/205C12R2001/465
Inventor 田春杰吉丽田磊张建峰谢金宏陈德国马丽娜
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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