Black fungus oligosaccharide for preventing liver cell oxidative damage as well as preparation method and application thereof

A technology of cell oxidation and fungus oligosaccharide, applied in biochemical equipment and methods, application, digestive system, etc., can solve the problem that ultrasonic-assisted extraction is noisy, cannot meet the large demand for industrial development and production of such products, enzyme preparations high cost issues

Pending Publication Date: 2020-10-20
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, acid and alkali will destroy the polysaccharide structure, and the addition of biological enzymes also limits the extraction conditions, which can only be carried out within the optimum temperature and pH range of the enzyme, and it needs to be inactivated and removed during subsequent separation and purification. The cost of the preparation is high (Li Xiao. Response surface method to optimize the conditions for enzymatic extraction of black fungus active polysaccharides [J]. Anhui Agricultural Sciences, 2010, 33: 475-478.); Ultrasound-assisted extraction is noisy (Xu Xiuhui. Ultrasonic method The technology of extracting black fungus polysaccharide[J]. Pharmacy and Clinical Research, 2011, 2(19): 189~190.); while microwave-assisted extraction is limited by equipment, the extraction amount is small (Zhu Lei. Response surface method to optimize microwave-assisted Research on Extraction Technology of Black Fungus Polysaccharide[J]. Chinese Journal of Food Science, 2009, 02: 58~65.)
None of the above-mentioned existing technologies can meet the large demand for industrialized development and production of such products.

Method used

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  • Black fungus oligosaccharide for preventing liver cell oxidative damage as well as preparation method and application thereof
  • Black fungus oligosaccharide for preventing liver cell oxidative damage as well as preparation method and application thereof
  • Black fungus oligosaccharide for preventing liver cell oxidative damage as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Pulverize the dried fruiting body of black fungus and pass through a 40-mesh sieve to form the dried fruiting body powder of black fungus. The dried fruiting body powder of black fungus is packed into a homogenizer, and deionized water is added according to the ratio of material to liquid at 1:80. Homogenize for 10 minutes, add 10 mg / mL pectinase solution to make the quality of the enzyme 0.4% of the dry fruiting body powder of black fungus, heat and react at a constant temperature in a 50°C water bath for 1 hour, then place the beaker in a 90°C water bath for 1 hour Hours, inactivate pectinase activity, squeeze filter. A single-screw extruder was used for extrusion filtration, the diameter of the die hole of the extruder was 16mm, the temperature of the extruder sleeve was 60°C, and the screw speed was 240r / min. At 90°C, the filtrate of black fungus will be concentrated to 1 / 3 of the original volume, cooled to room temperature, add 3 times the volume of absolute eth...

Embodiment 2

[0033] 1. Pulverize the dried fruiting body of black fungus and pass through a 40-mesh sieve to form the dried fruiting body powder of black fungus. The dried fruiting body powder of black fungus is packed into a homogenizer, and deionized water is added according to the ratio of material to liquid at 1:100. Homogenize for 5 minutes, add 10mg / mL pectinase solution to make the enzyme quality 0.4% of the dried fruit body powder of black fungus, heat and react in a water bath at 50°C for 1 hour, then place the beaker in a water bath at 100°C for 1 hour , inactivate the pectinase activity, and carry out extrusion filtration. A single-screw extruder was used for extrusion filtration, the diameter of the die hole of the extruder was 8mm, the temperature of the extruder sleeve was 80°C, and the screw speed was 160r / min. At 100°C, the filtrate of black fungus will be concentrated to 1 / 2 of the original volume, cooled to room temperature, add 3 times the volume of absolute ethanol, sta...

Embodiment 3

[0037] 1. Pulverize the dried fruiting body of black fungus and pass through a 40-mesh sieve to form the dried fruiting body powder of black fungus. Put the dried fruiting body powder of black fungus into the homogenizer, add deionized water according to the ratio of material to liquid at 1:30, Homogenize for 20 minutes, add 10 mg / mL pectinase solution to make the enzyme quality 0.4% of the dry fruiting body powder of black fungus, heat and react in a water bath at 50°C for 1 hour, then place the beaker in a water bath at 80°C for 1 hour , inactivate the pectinase activity, and carry out extrusion filtration. Extrusion filtration adopts a single-screw extruder, the diameter of the die hole of the extruder is 16mm, the temperature of the extruder sleeve is 100°C, and the screw speed is 260r / min. At 80°C, the black fungus filtrate will be concentrated to 1 / 3 of the original volume, cooled to room temperature, add 4 times the volume of absolute ethanol, stand at 4°C for 12h, 8000...

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Abstract

The invention relates to a black fungus oligosaccharide for preventing liver cell oxidative damage as well as a preparation method and application thereof, and belongs to the field of preparation of effective components of edible fungi. The black fungus oligosaccharide is prepared from dried black fungus fruit bodies. The preparation method comprises following steps: rapid homogenization in combination with enzymatic extraction; extrusion filtration and protein removal; and ion exchange chromatography. The preparation method has the advantages that the extraction time is shortened, the reagentconsumption is reduced, the energy is saved, the solubility is improved, and the adopted extrusion filtration method is suitable for separating a viscous solution with a high colloid content; meanwhile, the black fungus oligosaccharide has the function of preventing liver cell oxidative damage and can be used for preparing liver-protecting and liver-protecting auxiliary medicines and health-carefoods, basic data for development of black fungus deep-processed products is provided, and the black fungus oligosaccharide has important economic and market values.

Description

technical field [0001] The invention relates to black fungus oligosaccharides for protecting liver cells from oxidative damage, a preparation method and application thereof, and belongs to the field of natural products. Background technique [0002] The liver is the largest metabolic organ in the abdominal cavity of the human body, which plays a role in regulating immune tolerance, synthesizing proteins, and maintaining the balance of the body's internal environment. In my country, more than 400,000 patients die of liver disease every year, and liver injury is a common physiological state of many liver diseases. Staying up late, fatigue, drunkenness, viruses, drugs, genetics, etc. are all causes of liver damage, but liver damage caused by various inducements will be accompanied by peroxidative states such as oxidative stress and accumulation of lipid peroxides. Black fungus is the main edible fungus variety in my country, and its annual production and consumption both rank ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125A23L31/00A61P1/16A61P39/06
CPCC08B37/006C08B37/0003A23L31/00A23L33/125A61P1/16A61P39/06A23V2002/00A23V2200/32A23V2250/602A23V2250/618A23V2250/61A23V2250/608A23V2250/638A23V2300/14A23V2250/208A23V2300/10A23V2300/20C08L5/00C12Y302/01015
Inventor 王琦苏玲张倩董力冉美合日班
Owner JILIN AGRICULTURAL UNIV
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