Method for rapidly detecting CP4-EPSPS in transgenic plant

Abstract

The invention relates to a method for rapidly detecting CP4-EPSPS in transgenic plants, comprising the following steps: step 1, preparation of colloidal carbon; step 2, preparation of colloidal carbon-labeled antibodies suitable for pH conditions; step 3, antibody coating to The reaction membrane is used as the T line and the C line of the reaction membrane coated with the secondary antibody; step 4, preparation of colloidal carbon test strips; step 5, extracting protein from the plant to be tested, and preparing protein extract; step 6, testing the test strip The paper strip is inserted into the protein extract solution to react; Step 7, after an appropriate reaction time, judge the result with the naked eye. The present invention adopts the colloidal carbon pad labeled with polyclonal antibody E01, the nitrocellulose membrane coated with polyclonal antibody E01 and the nitrocellulose membrane coated with goat anti-rabbit IgG to make detection test strips; The principle of immunochromatography is to form antigen-antibody complexes to make the results visible to the naked eye.

Description

technical field [0001] The invention relates to a rapid detection method for protein CP4-EPSPS in grain bulk grains of transgenic rapeseed, soybean and other crops and rough processed products thereof, belonging to the field of transgenic detection. Background technique [0002] With the influx of various genetically modified foods into the market, the safety of genetically modified foods has attracted increasing attention. Countries around the world have great controversy over the safety of genetically modified foods. The European Union, Japan and other regions have formulated relevant laws and regulations to strictly manage genetically modified foods. Therefore, a method for rapidly detecting transgenic proteins in transgenic crops / plants is of great significance. [0003] Since Monsanto (Monsato) listed the first case of glyphosate-resistant soybeans in 1996, the promotion and application of genetically modified crops has gone through 18 years, and herbicide resistance i...

AI Technical Summary

Problems solved by technology

The PCR method can accurately detect genetically modified ingredients from crop seeds to crop maturity, and is not affected by sample processing methods, but it is prone to false positives and false negatives, and due to highly processed genetically modified products and Its derivatives, such as vegetable oil, sugar or starch, do not contain any DNA components, so PCR methods cannot detect such genetically modified foods

Enzyme-linked immunosorbent assay can process samples in batches, but it takes a long time, requires professional technicians, and requires special instruments. Therefore, this detection method is not conducive to the promotion and use of users without relevant professional skills.

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