Time resolution immunochromatographic test strip for quantitatively detecting pepsinogen I as well as preparation method of time resolution immunochromatographic test strip
An immunochromatographic test strip and pepsinogen technology, applied in the field of clinical immunology detection, can solve the problems of unsuitability for single-unit and small batch detection, inability to achieve accurate quantification, low sensitivity, etc., and is suitable for large-scale production, Simple operation and high sensitivity effect
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[0028] Example 1
[0029] The present invention is a time-resolved immunochromatographic test strip for quantitatively detecting pepsinogen I. The test strip includes a plastic cartridge, a bottom plate, and a sample pad, a binding pad, a coating film and a absorbent paper attached to the bottom plate and arranged in a staggered sequence. , the binding pad is coated with rare earth ion microsphere-labeled pepsinogen I monoclonal antibody I; the coating film is coated with a detection zone and a quality control zone, and the detection zone is fixed with different antigenic epitopes. Pepsinogen I monoclonal antibody II, rabbit anti-mouse IgG antibody was immobilized in the control zone.
[0030] The binding pad is a polyester film, which can carry a sufficient amount of rare earth ion microspheres, and can quickly release the microspheres after encountering the sample.
[0031] The coating film is a nitrocellulose film; the detection strip and the quality control strip coated o...
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[0048] Example 2
[0049] The present invention is a time-resolved immunochromatographic test strip for quantitatively detecting pepsinogen I. The test strip includes a plastic cartridge, a bottom plate, and a sample pad, a binding pad, a coating film and a absorbent paper attached to the bottom plate and arranged in a staggered sequence. , the binding pad is coated with rare earth ion microsphere-labeled pepsinogen I monoclonal antibody I; the coating film is coated with a detection zone and a quality control zone, and the detection zone is fixed with different antigenic epitopes. Pepsinogen I monoclonal antibody II, rabbit anti-mouse IgG antibody was immobilized in the control zone.
[0050] The binding pad is a polyester film, which can carry a sufficient amount of rare earth ion microspheres, and can quickly release the microspheres after encountering the sample.
[0051] The coating film is a nitrocellulose film; the detection strip and the quality control strip coated o...
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