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Plate-type chemiluminescence detection kit of cytokeratin 19-fragment and preparation method

A chemiluminescence method and cytokeratin technology are applied in the field of quantitative detection kits and preparations of cytokeratin 19 fragments, which can solve the problems of inappropriate use of tumor screening in the general population and the inability to diagnose or confirm the basis of early malignant tumors, etc. Achieve the effect of shortening production time, improving sensitivity, and reducing usage

Inactive Publication Date: 2017-12-12
JIANGSU FLON BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] This kit is mainly used for dynamic monitoring of patients with related malignant tumors to assist in judging the disease process or treatment effect. It cannot be used as the basis for early diagnosis or diagnosis of related malignant tumors, and it is not suitable for tumor screening in the general population.

Method used

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  • Plate-type chemiluminescence detection kit of cytokeratin 19-fragment and preparation method
  • Plate-type chemiluminescence detection kit of cytokeratin 19-fragment and preparation method
  • Plate-type chemiluminescence detection kit of cytokeratin 19-fragment and preparation method

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Embodiment Construction

[0071] The present invention will be described below in conjunction with specific examples. The given examples are only general illustrations of the products or methods of the present invention, which help to better understand the present invention, but do not limit the scope of the present invention. The experimental methods described in the following examples, unless otherwise specified, are conventional methods; the materials, unless otherwise specified, can be obtained from commercial sources.

[0072] The main reagents of the CYFRA21-1 plate type chemiluminescence detection kit in this embodiment include: the included reagents are biotin-labeled CYFRA21-1 antibody solution, anti-biotin antibody-coated ELISA plate, horseradish peroxidase Labeled CYFRA21-1 antibody solution, CYFRA21-1 calibrator A-F, concentrated washing solution, substrate solution A, substrate solution B.

[0073] Preparation method of the kit

[0074] First, the preparation of concentrated lotion, the s...

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Abstract

The invention relates to a plate-type chemiluminescence detection kit of a cytokeratin 19-fragment. The kit comprises the following reagents: biotin-marked CYFRA21-1 antibody solution, an elisa plate coated by anti-biotin, horse-radish-peroxidase-marked CYFRA21-1 antibody solution, a CYFRA21-1 calibration product A-F, concentrated washing liquor, substrate solution A, and substrate solution B. The main design idea of the detection kit is that the detection specificity of the kit is stabilized by introducing a biotin system with the anti-biotin on the basis of chemiluminescence immune assay, and the detection sensitivity and detection time are greatly increased. A high-temperature oscillatory-type anti-biotin coating technology is improved, so the usage amount of antibodies / antigens is reduced, the production flow of the reagents is simplified, and the production cycle is shortened.

Description

technical field [0001] The invention belongs to the field of biological kit materials, in particular to a quantitative detection kit for cytokeratin 19 fragments and a preparation method, which can quickly, easily, efficiently and sensitively quantify cytokeratin 19 fragments in human serum samples detection. Background technique [0002] Cytokeratin 19 fragment (Cytokeratin-19-fragment CYFRA21-1) is that when alveolar epithelial cells undergo apoptosis, the keratin fragments contained in the cells degrade and become soluble substances and enter the blood, increasing the blood content. [0003] CYFRA21-1 is the preferred marker for non-small cell lung cancer, especially for squamous cell carcinoma, with a sensitivity of 60% and a specificity of 95%. It is of great significance to the early diagnosis, curative effect monitoring and prognosis judgment of non-small cell lung cancer (NSCLC). CYFRA21-1 is often used to monitor the course and prognosis of non-small cell lung can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68G01N33/96G01N33/535G01N21/76
Inventor 戴宝平彭会军钱晓锦张伟
Owner JIANGSU FLON BIOTECH
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