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Hybridoma cell line capable of secreting foot and mouth disease virus sharable monoclonal antibody 3D9 and application of hybridoma cell line

A technology of hybridoma cell line and foot-and-mouth disease virus, applied in the application field of foot-and-mouth disease virus shared monoclonal antibody in the diagnosis or prevention of foot-and-mouth disease, which can solve the problems of reduced meat consumption, reduced animal production performance, and reduced meat products

Inactive Publication Date: 2017-05-31
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease causes the death of young animals, decreased milk production, reduced meat consumption, reduced meat production, and reduced animal production performance. It is also highly contagious and can form a large-scale epidemic, causing huge economic losses.

Method used

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  • Hybridoma cell line capable of secreting foot and mouth disease virus sharable monoclonal antibody 3D9 and application of hybridoma cell line
  • Hybridoma cell line capable of secreting foot and mouth disease virus sharable monoclonal antibody 3D9 and application of hybridoma cell line
  • Hybridoma cell line capable of secreting foot and mouth disease virus sharable monoclonal antibody 3D9 and application of hybridoma cell line

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Embodiment 1

[0021] Example 1 Preparation and identification of monoclonal antibodies shared by each serotype strain of FMDV

[0022] 1. Materials and methods

[0023] 1.1 Viruses, cells, strains and experimental animals

[0024] The strains used for IFA analysis of monoclonal antibody specificity include: A FMDV A / KT / 58 (GenBank accession number: AJ131665), A FMDV A / JLYS / CHA / 2014, A / XJBC / CHA / 2010, Asia1 FMDV Asia1 / YS / CHA / 05(GU931682), O / YS / CHA / 05(HM008917), O FMDV O / Tibet / CHA / 99(AJ539138), O / GD / 86(AJ131468)(Wang H, Zhao L, Li W , Zhou G, Yu L(2011) Identification of aconformational epitope on the VP1 G-H Loop of type Asia1 foot-and-mouth disease virus defined by a protective monoclonal antibody.Vet Microbiol 148:189-199.); Milk hamster kidney cells BHK- 21. SP2 / 0 myeloma cells were preserved in the inventor's laboratory; clean-grade female BALB / c mice were purchased from the Experimental Animal Center of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences. M...

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Abstract

The invention discloses a hybridoma cell line capable of secreting foot and mouth disease virus sharable monoclonal antibody 3D9 and application of the hybridoma cell line. Firstly, the invention discloses a strain of hybridoma cell line capable of secreting foot and mouth disease virus sharable monoclonal antibody 3D9, the microbial preservation No. is CGMCC No.13293. The invention further discloses the foot and mouth disease virus sharable monoclonal antibody 3D9 secreted by the hybridoma cell line. IFA specificity identification shows that the monoclonal antibody 3D9 is a strain of a serotype sharable monoclonal antibody which can react with O-type, A-type and Asia1-type foot and mouth disease virus antigens. In the invention, the monoclonal antibody 3D9 is used as a detection antibody and can be used for specific detection of O-type, A-type and Asia1-type foot and mouth disease virus antigens or antibodies. The hybridoma cell line and the foot and mouth disease virus sharable monoclonal antibody 3D9 secreted by the hybridoma cell line have an application prospect in preparation of reagents or drugs for diagnosing, preventing or treating foot-and-mouth diseases.

Description

technical field [0001] The present invention relates to a hybridoma cell line secreting a foot-and-mouth disease virus shared monoclonal antibody, and also relates to the application of the hybridoma cell line and the foot-and-mouth disease virus shared monoclonal antibody secreted by the hybridoma cell line in the diagnosis or prevention of foot-and-mouth disease, belonging to the field of prevention and treatment of foot-and-mouth disease . Background technique [0002] Foot and mouth disease virus (FMDV) is a member of the genus FMDV in the picornaviridae family. It is a single-stranded positive-sense RNA, and its genome RNA is about 8.5 kb in length. Foot-and-mouth disease (FMD) is caused by foot-and-mouth disease virus (FMDV), and it is a highly contagious infectious disease that mainly affects cattle, pigs, sheep and other livestock and a variety of wild cloven-hoofed animals. Humans can also be infected. The disease causes the death of young animals, decreased milk p...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/10A61K39/42A61P31/14G01N33/577G01N33/569
CPCC07K16/1009A61K39/00
Inventor 于力周国辉王海伟杨德成
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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