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Detection method for human erythrocyte rare blood type genotype and detection kit

A technology of genotyping and detection methods, applied in the field of biomedicine, can solve problems such as difficult genotyping of red blood cell rare blood types

Inactive Publication Date: 2019-08-02
为康(苏州)基因科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

Solved the problem of difficult genotyping of red blood cell rare blood types

Method used

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  • Detection method for human erythrocyte rare blood type genotype and detection kit
  • Detection method for human erythrocyte rare blood type genotype and detection kit

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Embodiment Construction

[0070] The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.

[0071] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0072] A detection method for human erythrocyte rare blood type genotyping of the present invention comprises the following steps:

[0073] 1) Extract sample DNA

[0074] 1. Centrifuge the sample in the centrifuge tube at 10,000 rpm for 2 min, remove the supernatant, and save the precipitate for later use.

[0075] 2. Add 350 μL Buffer MCL and 20 μL Proteinase K to the above centrifuge tube, vortex and mix well, place in a water bath at 65°C for 20 min, and mix well occasionally.

[0076] 3. After the water bath is completed, add 350 μL Buffer MA and 25 μL SanMag Beads to the centrifuge tu...

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Abstract

The invention provides a detection method for human erythrocyte rare blood type genotype and a detection kit. The detection method comprises the following steps: designing a specific primer and then utilizing single-tube multiple PCR amplified reaction, thereby acquiring a target segment; designing a single-base extension primer and then performing single-base extension; adopting mass spectrometryfor accurately measuring a target DNA sequence. The gene detection method is capable of simplifying a blood compatibility test and identifying rare blood type and is an alternative of a serotype identification method. The new method is capable of helping doctors to acquire a more accurate and comprehensive blood type result and helping patients to save cost.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a detection method and a detection kit for human erythrocyte rare blood group genotyping. Background technique [0002] There are 35 human blood group systems discovered so far, including not only the traditional ABO system, but also rare blood group systems such as Rh, Mumbai, P, Kell and MNS. Some people have rare blood types, especially in patients with long-term blood transfusions such as sickle cell anemia patients. If the blood type of the transfusion does not match, these people will have a transfusion reaction and may die in severe cases. Accurate detection and identification of rare blood types of red blood cells is of great significance for clinical medicine and blood transfusion practice. [0003] The present invention performs typing detection on human erythrocyte Rh, Kell, Duffy, Kidd, MNS, Lutheran, Dombrock, Landsteiner Wiener, Diego, Colton, Scianna, Cartwright 12 sam...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/686C12Q1/6876
CPCC12Q1/686C12Q1/6876C12Q2537/143C12Q2565/627
Inventor 王文忠田军龙张为胡军陈更新
Owner 为康(苏州)基因科技有限公司
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