Haemophilus parasuis disease antibody detecting test strip and preparation method thereof

A technology for Haemophilus suis disease and Haemophilus suis, which is applied in the field of molecular biology, can solve the problems of the limited application of diagnostic methods and the inability to meet the maximum demand for the diagnosis of Haemophilus parasuis

Inactive Publication Date: 2013-05-01
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF4 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a test strip for detecting antibodies against Haemophilus parasuis and a preparation method thereof, aiming at solving the problem that the prior art provides a method for diagnosing Haemophilus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Haemophilus parasuis disease antibody detecting test strip and preparation method thereof
  • Haemophilus parasuis disease antibody detecting test strip and preparation method thereof
  • Haemophilus parasuis disease antibody detecting test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0039] The preparation method comprises the following steps:

[0040] In step S401, colloidal gold is prepared by trisodium citrate reduction method;

[0041] In step S402, prepare colloidal gold-labeled purified and refolded His-OppA fusion protein;

[0042] In step S403, the colloidal gold pad and the nitrocellulose membrane 2 containing the detection line 7 and the quality control line 6 are prepared and assembled to form a test strip.

[0043] In the embodiment of the present invention, the realization method of preparing the purified refolded His-OppA fusion protein labeled with colloidal gold is as follows:

[0044] Adjust the pH value of the prepared colloidal gold to 7.0-7.8 with 0.1mol / L KCO3;

[0045] Determine the optimal amount of colloidal gold and the protein to be labeled by visual inspection, and obtain the optimal labeling amount as 2.4mg / 100ml, add the His-OppA fusion protein of purification and refolding by 2.4mg / 100ml in the colloidal gold solution, static ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a haemophilus parasuis disease antibody detecting test strip and a preparation method thereof. The haemophilus parasuis disease antibody detecting test strip consists of a PVC (polyvinyl chloride) lining plate, a nitrocellulose membrane, an absorbing pad, a gold label pad and a sample pad, wherein the PVC lining plate is arranged at the very bottom; the nitrocellulose membrane is arranged on the middle section of the upper part of the PVC lining plate; the absorbing pad is arranged at the left end of the upper part of the nitrocellulose membrane; the gold label pad is arranged at the right end of the upper part of the nitrocellulose membrane; the sample pad is arranged at the right end of the upper part of the gold label pad; an anti-haemophilus parasuis IgG is sprayed at the left end of the nitrocellulose membrane as a quality control line; a purified and renatured His-OppA fusion protein is sprayed at the right end of the nitrocellulose membrane as a detection line; and a colloidal gold-labeled purified and renatured His-OppA fusion protein is sprayed on the gold label pad. The haemophilus parasuis disease antibody detecting test strip can determine whether a haemophilus parasuis disease antibody exists in serum or not according to whether the detection line and the quality control line have color strips or not, and can detect all antibodies generated by a serotype haemophilus parasuis infection simply, conveniently and quickly.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a test strip for detecting antibodies against Haemophilus parasuis disease and a preparation method thereof. Background technique [0002] Haemophilus parasuis is an infectious disease of pigs characterized by fibrinous serositis, polyarthritis and meningitis caused by Haemophilus parasuis. The disease occurs all over the world, usually in pigs aged 5 to 8 weeks, with an incidence rate of 10% to 15%, and a mortality rate of up to 50% in severe cases. In recent years, the infection rate and the resulting mortality of this disease in large-scale pig farms have been increasing day by day, and it has become one of the more serious bacterial diseases that endanger the pig industry. [0003] Haemophilus parasuis is a worldwide conditional disease that is present in many pig farms. In recent years, my country's pig industry has developed rapidly, but the level of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/569G01N33/558G01N33/544
Inventor 逯忠新高鹏程储岳峰赵萍贺英
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap