Haemophilus parasuis disease antibody detecting test strip and preparation method thereof

Abstract

The invention discloses a haemophilus parasuis disease antibody detecting test strip and a preparation method thereof. The haemophilus parasuis disease antibody detecting test strip consists of a PVC (polyvinyl chloride) lining plate, a nitrocellulose membrane, an absorbing pad, a gold label pad and a sample pad, wherein the PVC lining plate is arranged at the very bottom; the nitrocellulose membrane is arranged on the middle section of the upper part of the PVC lining plate; the absorbing pad is arranged at the left end of the upper part of the nitrocellulose membrane; the gold label pad is arranged at the right end of the upper part of the nitrocellulose membrane; the sample pad is arranged at the right end of the upper part of the gold label pad; an anti-haemophilus parasuis IgG is sprayed at the left end of the nitrocellulose membrane as a quality control line; a purified and renatured His-OppA fusion protein is sprayed at the right end of the nitrocellulose membrane as a detection line; and a colloidal gold-labeled purified and renatured His-OppA fusion protein is sprayed on the gold label pad. The haemophilus parasuis disease antibody detecting test strip can determine whether a haemophilus parasuis disease antibody exists in serum or not according to whether the detection line and the quality control line have color strips or not, and can detect all antibodies generated by a serotype haemophilus parasuis infection simply, conveniently and quickly.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a test strip for detecting antibodies against Haemophilus parasuis disease and a preparation method thereof. Background technique [0002] Haemophilus parasuis is an infectious disease of pigs characterized by fibrinous serositis, polyarthritis and meningitis caused by Haemophilus parasuis. The disease occurs all over the world, usually in pigs aged 5 to 8 weeks, with an incidence rate of 10% to 15%, and a mortality rate of up to 50% in severe cases. In recent years, the infection rate and the resulting mortality of this disease in large-scale pig farms have been increasing day by day, and it has become one of the more serious bacterial diseases that endanger the pig industry. [0003] Haemophilus parasuis is a worldwide conditional disease that is present in many pig farms. In recent years, my country's pig industry has developed rapidly, but the level of...

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Problems solved by technology

[0006] The invention provides a test strip for detecting antibodies against Haemophilus parasuis and a preparation method thereof, aiming at solving the problem that the prior art provides a method for diagnosing Haemophilus parasuis, which needs professional equipment and technology, and these diagnostic methods The limitations of application are relatively large, and the problem of maximizing the diagnosis of Haemophilus parasuis cannot be met

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