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Carbon dioxide diagnosis/measuring reagent kit and carbon dioxide concentration determination method

A carbon dioxide and reagent technology, applied in the field of medical/food/environmental testing and determination, which can solve problems such as lack of experimental research

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] None of the above patents has anything to do with enzymatic determination, which shows from one side that there is a lack of experimental research in this area in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The carbon dioxide diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0046] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Reduced coenzyme 0.25mmol / L

[0049] Phosphoenolpyruvate Carboxylase 6000U / L

[0050] Malate dehydrogenase 10000U / L

[0051] Phosphoenolpyruvate 6mmol / L

[0052] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0053]Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sample to the reagent is 1 / 25, The reaction direction is negative reaction (falling reaction), the delay time is 0 minutes, and the detection time is about 5 minutes.

[0054] After add...

Embodiment 2

[0056] The carbon dioxide diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0057] Reagent 1

[0058] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0059] Stabilizer 50mmol / L

[0060] Reduced coenzyme 0.25mmol / L

[0061] Phosphoenolpyruvate 6mmol / L

[0062] Reagent 2

[0063] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0064] Stabilizer 500mmol / L

[0065] Phosphoenolpyruvate Carboxylase 6000U / L

[0066] Malate dehydrogenase 10000U / L

[0067] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0068] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative r...

Embodiment 3

[0071] The carbon dioxide diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0072] Reagent 1

[0073] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 50mmol / L

[0075] Reduced coenzyme 0.25mmol / L

[0076] Phosphoenolpyruvate 6mmol / L

[0077] Reagent 2

[0078] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0079] Stabilizer 500mmol / L

[0080] Malate dehydrogenase 10000U / L

[0081] Reagent 3

[0082] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 500mmol / L

[0084] Phosphoenolpyruvate Carboxylase 6000U / L

[0085] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0086] When measuring carbon dioxide concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wav...

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Abstract

The invention relates to a kit for diagnosing / measuring carbon dioxide by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the carbon dioxide, and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, a phosphoenolpyruvic acid, phosphoenolpyruvate carboxykinase, malic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the carbon dioxide.

Description

technical field [0001] The invention relates to a carbon dioxide diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring carbon dioxide concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] The content of carbon dioxide in the blood plays an important role in the regulation of acid-base balance in the human body. The constant blood pH is an essential condition for maintaining life activities. Among the various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH of blood. [0003] Changes in the content of carbon dioxide in the blood mainly reflect metabolic acid-base balance disorders. In pure metabolic acid or alkalosis, blood bicarbonate [HCO 3 - ] decreased or increased, the total carbon dioxide also decreased or increased. In simple respiratory acid or alkalosis, blood bicar...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/84C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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