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CO2 content determination method and CO2 diagnosis kit

A diagnostic kit and carbon dioxide technology, applied in the field of medical testing and measurement, can solve the problems of lack of experimental research and achieve the effect of accurate test results and good stability

Inactive Publication Date: 2006-04-26
王尔中
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above patents have nothing to do with enzymatic determination, which shows from one aspect that domestic experimental research in this area is very lacking

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment one (single dose)

[0095] The carbon dioxide (bicarbonate) diagnostic kit of the present embodiment comprises:

[0096] Buffer 80mmol / l

[0097] Phosphoenolpyruvate 1mmol / l

[0098] Inosine 1mmol / l

[0099] Phosphoenolpyruvate Carboxylase 6000U / l

[0100] Nucleoside phosphatase 6000U / l

[0101] Xanthine oxidase 5000U / l

[0102] Ethanol 5mmol / l

[0103] Catalase 5000U / l

[0104] Oxidized coenzyme 2mmol / l

[0105] Aldehyde dehydrogenase 5000U / l

[0106] Stabilizer 50% (total volume)

[0107] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the measured carbon dioxide (bicarbonate) sample to the reagent is 1 / 25, the reaction Direction is positive reaction.

[0108] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0109] carbon dioxide + phosphoenolpyruvate phosphoenolpyruvate carboxylase ...

Embodiment 2

[0117] Embodiment two (two doses)

[0118] The carbon dioxide (bicarbonate) diagnostic reagent of the present embodiment has:

[0119] Reagent I

[0120] Buffer 100mmol / l

[0121] Inosine 3mmol / l

[0122] Nucleoside phosphatase 13000U / l

[0123] Xanthine oxidase 12500U / l

[0124] Ethanol 10mmol / l

[0125] Catalase 10000U / l

[0126] Oxidized coenzyme 4mmol / l

[0127] Aldehyde dehydrogenase 12500U / l

[0128] Stabilizer 50% (total volume)

[0129] Reagent II

[0130] Buffer 100mmol / l

[0131] Phosphoenolpyruvate 6mmol / l

[0132] Phosphoenolpyruvate Carboxylase 8000U / l

[0133] Stabilizer 50% (total volume)

[0134] When measuring the content of carbon dioxide (bicarbonate), the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 340nm, the secondary wavelength of the test is above 405nm, and the volume ratio of the measured carbon dioxide (bicarbonate) sample to the reagent is 1 / 25 , and the reaction direction is po...

Embodiment 3

[0144] Embodiment three (three doses)

[0145] The carbon dioxide (bicarbonate) diagnostic reagent of the present embodiment is three doses, has:

[0146] Reagent I

[0147] Buffer 120mmol / l

[0148] Inosine 5mmol / l

[0149] Ethanol 15mmol / l

[0150] Oxidized coenzyme 6mmol / l

[0151] Stabilizer 20mmol / l

[0152] Reagent II

[0153] Buffer 120mmol / l

[0154] Phosphoenolpyruvate Carboxylase 10000U / l

[0155] Nucleoside phosphatase 20000U / l

[0156] Xanthine oxidase 20000U / l

[0157] Catalase 15000U / l

[0158] Aldehyde dehydrogenase 20000U / l

[0159] Stabilizer 50% (total volume)

[0160] Reagent III

[0161] Buffer 120mmol / l

[0162] Phosphoenolpyruvate 10mmol / l

[0163] Stabilizer 20mmol / l

[0164] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, volume ratio of measured carbon dioxide (bicarbonate) sample to reagent is 1 / 25, reaction Direction is positive reacti...

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Abstract

The invention discloses a detection method and diagnosis reagent box of carbon dioxide (bicarbonate) in the medical detection domain, which comprises the following parts: buffer liquid, phosphoenolpyruvate, muscle nucleoside, phosphoenolpyruvate carboxylase, nucleoside phosphatase, xanthine oxidase, alcohol, hydrogen peroxidase, oxidized coenzyme, aldehydo dehydrogenase and stabilizer. The detection method comprises the following steps: mixing the sample and reagent at certain bulk proportion to do enzyme coupling reaction; setting the final reactant under the biochemical analyzer; detecting main wavelength absorbance variation condition (speed); counting the content of carbon dioxide (bicarbonate).

Description

technical field [0001] The invention relates to a method for measuring carbon dioxide (bicarbonate) content, and simultaneously the invention also relates to a carbon dioxide (bicarbonate) diagnostic kit, which belongs to the technical field of medical examination and measurement. Background technique [0002] The content of carbon dioxide in the blood plays an important role in the regulation of acid-base balance in the human body. The constant blood pH is an essential condition for maintaining life activities. Among the various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH of blood. [0003] Changes in the content of carbon dioxide in the blood mainly reflect metabolic acid-base balance disorders. In pure metabolic acid or alkalosis, blood bicarbonate [HCO 3 - ] decreased or increased, the total carbon dioxide also decreased or increased. In simple respiratory acid or alkalosis, blood bicarbonate rise...

Claims

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Application Information

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IPC IPC(8): G01N33/70G01N33/50G01N21/75G01N21/77G01N21/33G01N21/31
Inventor 王尔中
Owner 王尔中
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