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Lithium diagnosis/measuring reagent kit and lithium concentration determination method

A technology of kits and reagents, applied in the field of lithium concentration determination, lithium diagnosis/measurement kits, can solve the problems of no experimental methods and experimental operating procedures, finding usage conditions, and difficulty in psychiatric hospitals, etc.

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic clinical laboratories do not have uniform experimental methods and experimental operating procedures, and it is difficult to find its usage in laboratories of psychiatric hospitals.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The lithium diagnosis / determination reagent of the present embodiment is a single reagent, comprising:

[0046] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Reduced coenzyme 0.25mmol / L

[0049] Inositol-1-phosphatase 10000U / L

[0050] Phosphoenolpyruvate Carboxylase 10000U / L

[0051] Malate dehydrogenase 10000U / L

[0052] Magnesium chloride 0.6mmol / L

[0053] Inositol-1-phosphate 8mmol / L

[0054] Oxaloacetic acid 8mmol / L

[0055] Pyruvate 20mmol / L

[0056] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured control and lithium sample to the reagent is 1 / 25. T...

Embodiment 2

[0060] The lithium diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0061] Reagent 1

[0062] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] Reduced coenzyme 0.25mmol / L

[0065] Magnesium chloride 0.6mmol / L

[0066] Inositol-1-phosphate 8mmol / L

[0067] Oxaloacetic acid 8mmol / L

[0068] Pyruvate 20mmol / L

[0069] Reagent 2

[0070] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 500mmol / L

[0072] Inositol-1-phosphatase 10000U / L

[0073] Phosphoenolpyruvate Carboxylase 10000U / L

[0074] Malate dehydrogenase 10000U / L

[0075] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0076] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary w...

Embodiment 3

[0079] The lithium diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0080] Reagent 1

[0081] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0082] Stabilizer 50mmol / L

[0083] Reduced coenzyme 0.25mmol / L

[0084] Magnesium chloride 0.6mmol / L

[0085] Inositol-1-phosphate 8mmol / L

[0086] Oxaloacetic acid 8mmol / L

[0087] Pyruvate 20mmol / L

[0088] Reagent 2

[0089] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Phosphoenolpyruvate Carboxylase 10000U / L

[0092] Malate dehydrogenase 10000U / L

[0093] Reagent 3

[0094] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 500mmol / L

[0096] Inositol-1-phosphatase 10000U / L

[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0098] When measuring lithium concentrati...

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Abstract

The invention relates to a kit for diagnosing / measuring lithium by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay, wherein, the activity of the kit can be inhibited by lithium. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of lithium, and belongs to the technical field of medical / industrial / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, magnesium chloride, inositol-1-phosphate, an oxaloacetic acid, a pyruvic acid, inositol-1-phosphatase, phosphoenolpyruvate carboxykinase, malic dehydrogenase and a stabilizer. Through mixing a check sample and a lithium sample respectively with the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, the degree / velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, and the difference in the degree / velocity of the decrease between the check sample and lithium sample is compared, thereby measuring the concentration of lithium.

Description

technical field [0001] The invention relates to a lithium diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring lithium concentration, which belongs to the technical field of medical / industrial / environmental inspection and determination. Background technique [0002] In the 1940s, Cade successfully used lithium salts to treat mania for the first time, with a total effective rate of about 70%. Lithium salts have a preventive effect on the recurrence of mania and depression, and are also used to treat schizoaffective psychosis. In addition, lithium salts can reduce suicidal behavior in the maintenance treatment phase of bipolar disorder by 86%, and when lithium salts are stopped, the risk of suicide will increase by 7.5 times. When the blood lithium concentration reaches or exceeds 1.5mmol / L, various degrees of poisoning symptoms will appear, and the blood lithium concentration above 3.0mmol / L can be life-threatening. [0003] I...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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