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DNA (Deoxyribonucleic Acid) molecule related to photosynthesis and application thereof

A DNA molecule, purpose technology, applied in recombinant DNA technology, DNA/RNA fragments, applications, etc., can solve the problems of low expression level and different tissue specificity, and achieve the effect of high photosynthetic efficiency

Active Publication Date: 2011-04-06
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

C4 plants evolved from C3 plants, so various key enzymes of the C4 pathway also exist in C3 plants, but the expression levels in C3 plants are lower and tissue specificity is different

Method used

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  • DNA (Deoxyribonucleic Acid) molecule related to photosynthesis and application thereof
  • DNA (Deoxyribonucleic Acid) molecule related to photosynthesis and application thereof
  • DNA (Deoxyribonucleic Acid) molecule related to photosynthesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, the acquisition of double T-DNA, multigene plant expression vector pCDMAR-PKMHT-NptII

[0047] 1. Establishment of double T-DNA multi-gene assembly vector system

[0048] 1) Accept the construction of vector pCDMAR-key-NptII

[0049] Design PCR primers: 5'-GGAATTCCaggatccaagcttgtcg-3'; 5'-GCTCTAGAaaacactgatagtttaaac-3', with plasmid pYLTAC747N (Lin, L., Liu, Y.G., Xu, X., and Li, B. (2003). Efficient linking and transfer of multiple genes by a multigene assembly and transformation vector system.Proc.Natl.Acad.Sci.100, 5962-5967, the public can obtain from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences.) as a template for PCR amplification, The obtained PCR product is the key sequence containing the loxP site and homing endonuclease I-Sce I.

[0050] Digest the above-mentioned PCR product with EcoR I and Xba I, and reclaim the key sequence of 200bp, and remove the selectable marker plasmid pCDMAR-hyg obtained by digesting...

Embodiment 2

[0124] Example 2, the acquisition and functional research of PKMHT tobacco

[0125] 1. Obtaining PKMHT Tobacco

[0126] 1. Agrobacterium-mediated genetic transformation of tobacco

[0127] 1) Preparation of tobacco sterile vaccine

[0128] Take an appropriate amount of tobacco (Nicotiana tabacum cv. Xanthi, Wang Zhuanmei et al., 2009. Cloning and bioinformatics analysis of the basic β-1,3-glucanase gene of tobacco variety Xanthi NN. Jiangsu Agricultural Science, 1:28- 31. The public can obtain it from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences.) The seeds are soaked in double distilled water at 37°C for 3-4h, sterilized with 30% (mass percentage) sodium hypochlorite aqueous solution for 8-10min, and placed in an ultra-clean bench Rinse with sterile water for 3-5 times, put them on sterile filter paper to dry naturally, and then transfer to MS medium at 25°C with a light / dark cycle of 16 / 8h for germination. After the cotyledons are fully...

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Abstract

The invention discloses a DNA (Deoxyribonucleic Acid) molecule related to photosynthesis and the application thereof. The DNA molecule is formed by sequentially connecting the following DNA segments: a DNA segment 1, a DNA segment 2, a DNA segment 3, a DNA segment 4 and a DNA segment 5. The DNA segment 1 is formed by sequentially connecting a promoter for driving PEPC (phosphoenolpyruvate carboxykinase) protein expression, an encoding gene of PEPC protein and a terminator; and the DNA segment 2 is formed by sequentially connecting a promoter for driving PPDK (Pyruvate Orthophosphate Dikinase)protein expression, an encoding gene of PPDK protein and a terminator. An experiment proves that a transgenic plant obtained by guiding a plurality of C4 pathway key enzyme bases into the C3 plant has higher photosynthetic efficiency than a wild plant (a C3 plant). The invention has a certain reference for practical operation for increasing crop photosynthetic efficiency by using a multigene transformation technology from now on.

Description

technical field [0001] The invention relates to a DNA molecule related to photosynthesis and its application, in particular to a DNA molecule and its application. Background technique [0002] Photosynthesis of plants refers to the biochemical process in which plants use chlorophyll to convert carbon dioxide and water into organic matter and release oxygen under the irradiation of visible light. Photosynthesis is divided into two stages: light reaction and dark reaction. The photoreaction takes place on the thylakoid membrane, using sunlight to split water, release oxygen, and generate high-energy phosphate (ATP) and reduced coenzyme II (NADPH). The dark reaction is carried out in the matrix, and the ATP formed by the light reaction is used to convert CO 2 reduced to sugar. Initially, according to the carbon assimilation pathway in photosynthesis, CO 2 The difference in fixed initial photosynthetic products divides higher plants into C3 plants, C4 plants and sedum acid (...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 朱祯陈斌
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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