Preparation method of nemadectin

A technology for fermenting and cultivating nimoctine, which is applied in the field of preparation of high-purity nimoctine and antibiotics. It can solve the problems of increased cost of nimoctine extraction, low purity of nimoctine, and a large amount of filtered waste water, etc., and achieves The effect of low cost, significant economic benefits and simple process
CN102336796BActive Publication Date: 2014-05-07CHONGQING DAXIN PHARMA +2
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHONGQING DAXIN PHARMA
Publication Date
2014-05-07

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a preparation method of nemadectin. According to the invention, a fermentation broth used for producing nemadectin is directly dried by spraying, such that solid powder is obtained; the solid powder is extracted by using an organic solvent, the extract is filtered, and nemadectin in the extract is absorbed by using macroporous resin; nemadectin absorbed by macroporous resin is then eluted; and the eluent is extracted, concentrated, and dried, such that a nemadectin pure product is obtained. According to the invention, a direct spray-drying method is adopted upon the fermentation broth, and the obtained dried solid fermentation bacterium powder is transportation-tolerant, and is easy to store. With the method provided by the invention, the producing of nemadectin degradation product is reduced, the bacterium powder is easy to extract, and the consumption of the extraction solvent is low. The technology is simple, the cost is low, and the method is easy to operate. With the method, the prepared nemadectin has a high HPLC purity of above 90%.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of animal medicines, and relates to a method for preparing antibiotics, in particular to a method for obtaining high-purity nemoctine by fermenting and cultivating nimoctine-producing bacteria, and then purifying the fermentation product. Background technique

[0002] Nemadectin is a sixteen-membered macrolide antibiotic produced by Streptomycescyaneogriseus sp.Noncyanogenus through fermentation. As a member of the milbemycin family, nimoctine is mainly used in the synthesis of the more active anthelmintic drug moxidectin.

[0003] European patent EP0170006A2 discloses a production medium and fermentation process for nemoctine. First, inoculate Streptomyces blue gray in a culture medium containing 1.0% glucose, 2.0% dextrin, 0.5% yeast extract, 0.5% NZ amine (enzymatic hydrolyzate of casein) and 0.1% calcium carbonate (note: each The unit of percentage content of a substance is g / mL, for example, 0.1% is 0.001g / mL, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More