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Method for detecting nemadectin content based on liquid chromatography external standard method

A liquid chromatograph, nimoctine technology, applied in the detection field of nimoctine content, can solve the problems such as the content of nimoctine is not reflected

Inactive Publication Date: 2017-06-27
HEBEI SHENGXUE DACHENG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection of nemoctine content is not reflected in the pharmacopoeias of China, EP and USP

Method used

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  • Method for detecting nemadectin content based on liquid chromatography external standard method
  • Method for detecting nemadectin content based on liquid chromatography external standard method
  • Method for detecting nemadectin content based on liquid chromatography external standard method

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Embodiment Construction

[0031] The technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0032] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

[0033] The present inve...

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Abstract

The invention discloses a method for detecting nemadectin content based on a liquid chromatography external standard method and specifically relates to the detection for the nemadectin content. The method provided by the invention comprises the following steps: precisely weighting a nemadectin sample and a nemadectin reference substance in same weight and placing into two volumetric flasks, respectively adding 95% ethyl alcohol solution and fixing the volume to a same scale, uniformly mixing and preparing into a sample solution and a reference solution, and then quantitatively and precisely weighting the sample solution and the reference solution acquired in the step a in same volume and performing high performance liquid chromatography determination, wherein the chromatographic conditions are as follows: octadecyl silane bonded silica gel column selected as a chromatographic column, column temperature at 25-35 DEG C, 85% methyl alcohol as flowing phase, flow rate at 1.0-1.5mL / min, detection wavelength of 240nm and sampling volume at 20uL. According to the invention, the nemadectin content can be accurately detected, the accuracy is high, the repeatability is high, the linear relation coefficient can reach up to 0.99, the accuracy RSD is less than or equal to 2.0%, the sensitivity is high and the result is satisfying.

Description

technical field [0001] The invention belongs to the detection of the content of nimoctine, and in particular relates to a method for detecting the content of nimoctine based on liquid chromatography. Background technique [0002] Nemadectin is a sixteen-membered macrolide antibiotic produced by fermentation of Streptomyces cyaneogriseus sp.Noncyanogenus. As a member of the milbemycin family, it exhibits good antiparasitic activity. As an antiparasitic antibiotic, nimoctine has a broad insecticidal spectrum, is easy to degrade, has low residue, no drug resistance, and is safe for humans and animals. Nimoctine is mainly used to synthesize Moxidectin, a more active pesticide and veterinary drug. [0003] At present, the detection of nimoctine content is not reflected in the pharmacopoeias of China, EP and USP. As a moxictine raw material, the detection of the content of nemoctine and a suitable and effective detection method are particularly important. Contents of the inven...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 杜石磊
Owner HEBEI SHENGXUE DACHENG PHARMA
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