Method for improving fermentation yield of nemadectin

A technology for improving the yield of nimoctine, which is applied in the field of increasing the yield of fermented nemoctine, can solve the problems of the yield to be increased and the low yield of nemoctine, and achieve the effect of increasing the yield and increasing the fermented yield

Active Publication Date: 2015-03-25
HEBEI SHENGXUE DACHENG PHARMA
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Problems solved by technology

[0004] The present invention is to solve the technical problem that the yield of nimoctine is low in the prior art, and the yield at the end of fermentation needs to be improved, and provides a method for improving the fermented yield of nimoctine

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  • Method for improving fermentation yield of nemadectin

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Embodiment 115

[0023] In embodiment 1.1.5T fermentor fermentation process, do not add sodium acetate

[0024] A. Select the starting strain: select a sodium acetate-resistant Streptomyces chromogenes mutant strain, and the taxonomic name of the sodium acetate-resistant Streptomyces chromogenes mutant strain is Streptomyces cyanneorisens DC18-01 , preserved in the China Microorganism Culture Collection Management Committee, the preservation number is CGMCC No.6217, and the preservation date is June 14, 2012;

[0025] B. Strain slant culture: Inoculate Streptomyces cyanneorisens DC18-01 on the slant medium, and culture it at 26-30°C and 30%-60% humidity for 96-144h to obtain the slant strain;

[0026] C. Strain bottle culture: dig 2-5cm 2 The slant strain prepared in step B is inoculated in the medium of the seed bottle, and cultured with shaking at 26-30°C and 220-240rpm for 24-30h to obtain a mature seed solution;

[0027] D, seed tank culture: inoculate the mature seed solution obtained i...

Embodiment 215

[0029] Example 2.1.5T fermenter fermentation process added sodium acetate 168h after the start of fermentation

[0030] A. Select the starting strain: select a sodium acetate-resistant Streptomyces chromogenes mutant strain, and the taxonomic name of the sodium acetate-resistant Streptomyces chromogenes mutant strain is Streptomyces cyanneorisens DC18-01 , preserved in the China Microorganism Culture Collection Management Committee, the preservation number is CGMCC No.6217, and the preservation date is June 14, 2012;

[0031] B. Strain slant culture: Inoculate Streptomyces cyanneorisens DC18-01 on the slant medium, and culture it at 26-30°C and 30%-60% humidity for 96-144h to obtain the slant strain;

[0032] C. Strain bottle culture: dig 2-5cm 2 The slant strain prepared in step B is inoculated in the medium of the seed bottle, and cultured with shaking at 26-30°C and 220-240rpm for 24-30h to obtain a mature seed solution;

[0033] D, seed tank culture: inoculate the mature...

Embodiment 312

[0035] Example 3. In the fermentation process of 12T fermenter, sodium acetate A was added 168h and 216h after the start of fermentation, and the starting strain was selected: a sodium acetate-resistant Streptomyces chromogenes mutant strain was selected, and the sodium acetate-resistant color-producing strain was selected. The taxonomic name of the Streptomyces mutant strain is Streptomyces cyanneorisens DC18-01, which was deposited in the China Committee for the Collection of Microorganisms with a preservation number of CGMCC No.6217 and a preservation date of June 14, 2012;

[0036] B. Strain slant culture: Inoculate Streptomyces cyanneorisens DC18-01 on the slant medium, and culture it at 26-30°C and 30%-60% humidity for 96-144h to obtain the slant strain;

[0037] C. Strain bottle culture: dig 2-5cm 2 The slant strain prepared in step B is inoculated in the medium of the seed bottle, and cultured with shaking at 26-30°C and 220-240rpm for 24-30h to obtain a mature seed so...

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Abstract

The invention discloses a method for improving fermentation yield of nemadectin. The method comprises the following steps: A. selecting an original strain; B. carrying out slant culture to the strain; C. bottling and cultivating the strain; D. cultivating the strain in a seeding tank; and E. cultivating the strain in a fermentation tank. By refilling sodium acetate for one or more times in the middle and later periods of fermentation, the method for improving the fermentation yield of the nemadectin disclosed by the invention can be used for greatly improving the yield of the nemadectin.

Description

technical field [0001] The invention relates to the fermentative production of nemoctine, in particular to a method for increasing the fermentative yield of nemoctin. Background technique [0002] Nimoctine is a sixteen-membered ring macrolide antibiotic produced by the fermentation of Streptomyces chromogenes or Streptomyces blue gray. It is mainly used to synthesize moxictine with stronger lipophilicity. Nimoctine is mainly used to synthesize Moxidectin, a more active pesticide and veterinary drug. Moxictine has been used as a veterinary anthelmintic since the mid-1980s. It can efficiently kill nematodes and ectoparasites, and has good safety for animals. It is currently widely used in veterinary clinics. Broad-spectrum, high-efficiency, new macrolide anthelmintic antibiotic. [0003] The yield of nimoctine in the prior art is low, which is far from meeting the industrial demand. In the prior art, methods for improving nimoctine include selecting high-yield strains, sup...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/465
Inventor 李明杨丽锋
Owner HEBEI SHENGXUE DACHENG PHARMA
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