Method for producing Nemadectin through fermenting

A technology of nimoctine and fermented liquid, which is applied in the field of microbial fermentation to produce antibiotics, can solve the problems that the synthesis process of fermented products is easily blocked and the strains are slow, and achieves the goals of simple and easy operation method, improved production level and reduced cost Effect

Active Publication Date: 2013-03-06
NEW FOUNDER HLDG DEV LLC +2
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  • Abstract
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  • Claims
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Problems solved by technology

The initial sugar concentration of the current fermentation production process is high, the bacteria enter the secondary metabolism process slowly, the synthesis process of the fermentation product is easily blocked, and the mycelium cracking is too fast in the middle and late stages of fermentation, and the yield at the end of the fermentation needs to be improved (Zhu Wan Yi, Luo Hongyu, Zhang Saiping, et al. Mutation Breeding of Nimoctine-producing Bacteria [J]. Biology Journal, 2008, 25(2): 62)

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  • Method for producing Nemadectin through fermenting

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Embodiment 1

[0021] Intermittent feeding in the fermentation process of embodiment 1 500L fermenter

[0022] Prepare 30L seed medium in a 50L seed tank, cool to 27-29°C after sterilizing at 120°C for 30min, insert 48h seed-age shake flask seeds according to 0.5% (volume ratio) inoculum, and then cultivate at 27-29°C for 28 -36h.

[0023] Prepare 350L fermentation medium in a 500L fermenter, cool to 27-29°C after sterilizing at 120°C for 30 minutes, insert the cultured seed medium according to 5% (volume ratio) inoculum, control the temperature at 27-27°C during the fermentation process 29°C, control the pressure at 0.04-0.06Mpa, control the ventilation and rotation speed according to the dissolved oxygen condition to ensure that the dissolved oxygen is above 30% (assuming that the initial dissolved oxygen of the fermenter is 100%), the ventilation is controlled at 0.5-1VVM, and the rotation speed is controlled at 120-250rpm, add ammonia water and 10% dilute sulfuric acid to control the pH...

Embodiment 2

[0024] Continuous feeding in the fermentation process of embodiment 2 5000L fermenter

[0025] Prepare 300L seed culture medium in a 500L seed tank, cool to 27-29°C after sterilizing at 120°C for 30 minutes, insert 48h seed-age shake flask seeds according to 0.5% (volume ratio) inoculum, and then cultivate at 27-29°C for 28 -36h.

[0026] Prepare 3500L fermentation medium in a 5000L fermenter, sterilize at 120°C for 30 minutes, then cool to 27-29°C, insert the seed medium at 5% (volume ratio) inoculum, control the temperature at 27-29°C during the fermentation process, Control the pressure at 0.04-0.06Mpa, control the ventilation and rotation speed according to the dissolved oxygen condition to ensure that the dissolved oxygen is above 30% (assuming that the initial dissolved oxygen of the fermenter is 100%), the ventilation is controlled at 0.5-1VVM, and the rotation speed is controlled at 90-180rpm. During the cultivation process, ammonia water and 10% dilute sulfuric acid ...

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Abstract

The invention discloses a method for producing Nemadectin through fermenting. In the Nemadectin production process through fermenting, carbohydrates are intermittently or continuously added to a fermentation liquid to maintain the total sugar concentration of the fermentation liquid at 10-40g / L in order to carry out fermenting production when the total sugar concentration of the fermentation liquid is less than 30g / L. The method enables the Nemadectin fermentation unit to be improved by above 50%, simultaneously has the advantages of simple production operation, low equipment requirements and low cost, and is a technology suitable for the industrialized production.

Description

technical field [0001] The invention relates to the technical field of microbial fermentative production of antibiotics, in particular to the fermentative production of nimoctine. More specifically, it relates to a method for cultivating Streptomyces coeruleus by continuously or intermittently adding high-concentration monosaccharide, disaccharide or polysaccharide solution to the fermentation broth to produce high-concentration nemoctin. Background technique [0002] Nemadectin is a sixteen-membered macrolide antibiotic produced by Streptomycescyaneogriseus sp.Noncyanogenus through fermentation. As a member of the milbemycin family, nimoctine is mainly used to synthesize Moxidectin, a more active pesticide and veterinary drug. Moxictine has been used as a veterinary anthelmintic since the mid-1980s. It can efficiently kill nematodes and ectoparasites, and has good safety for animals. It is currently widely used in veterinary clinics. Broad-spectrum, high-efficiency, new m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/465
Inventor 别一赵德刘会明
Owner NEW FOUNDER HLDG DEV LLC
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