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Method for improving nemadectin yield

A technology of nimoctine and yield, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as large environmental impact, environmental pollution, and low safety

Active Publication Date: 2016-06-01
牡丹江佰佳信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Organic chemical pesticides were invented in the 1940s. Its appearance has greatly enhanced the ability of humans to control diseases and insect pests, and has made a major contribution to the recovery of crop yield losses. However, organic chemical pesticides generally have high toxicity and low safety. It has a great impact on the environment. Its long-term and extensive use has brought about a series of problems such as environmental pollution, ecological damage and food safety, which have seriously affected the sustainable development of the agricultural economy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 improves the method for nimoctin production

[0018] Slope culture, seed culture and fermentation culture of Streptomyces licheniformis BJX003.

[0019] Fermentation culture method: take the seed culture liquid and inoculate it in a 50L fermentation tank equipped with 25L fermentation medium according to the inoculation amount of 5% (v / v), at 28°C, rotating speed 200rpm, ventilation ratio 1:0.5, tank pressure 0.03MPa , under the condition that the dissolved oxygen is controlled at more than 40%, the fermentation broth is fermented for 240 hours to obtain the fermentation broth. During the fermentation process, when the concentration of reducing sugar in the fermentation broth is lower than 15g / L, add a glucose solution containing soybean meal powder, and control the concentration of reducing sugar at 15g / L; wherein the concentration of glucose in the feed solution is 350g / L, the concentration of soybean flour is 100g / L, prepared with water.

[0020] The f...

Embodiment 2

[0021] Embodiment 2 improves the method for nimoctin production

[0022] Slope culture, seed culture and fermentation culture of Streptomyces licheniformis BJX003.

[0023] Fermentation culture method: take the seed culture liquid and inoculate it in a 50L fermenter equipped with 25L fermentation medium according to the inoculation amount of 5% (v / v), at 26 ° C, the rotating speed is 180rpm, the ventilation ratio is 1:0.3, and the tank pressure is 0.04MPa. , under the condition that the dissolved oxygen is controlled at more than 40%, the fermentation broth is fermented and cultured for 192 hours to obtain the fermentation broth. During the fermentation process, when the concentration of reducing sugar in the fermentation broth is lower than 12g / L, add a glucose solution containing soybean meal powder, and control the concentration of reducing sugar to 12g / L; wherein the concentration of glucose in the feed solution is 300g / L, the concentration of soybean flour is 75g / L.

[...

Embodiment 3

[0025] Embodiment 3 improves the method for nimoctin production

[0026] Slope culture, seed culture and fermentation culture of Streptomyces licheniformis BJX003.

[0027] Fermentation culture method: take the seed culture liquid and inoculate it in a 50L fermenter equipped with 25L fermentation medium according to the inoculation amount of 5% (v / v), at 30°C, rotating speed 220rpm, ventilation ratio 1:0.7, tank pressure 0.03MPa , under the condition that the dissolved oxygen is controlled above 40%, the fermentation broth is obtained by fermenting and culturing for 264 hours. During the fermentation process, when the concentration of reducing sugar in the fermented liquid is lower than 18g / L, add a glucose solution containing soybean meal powder, and control the concentration of reducing sugar at 18g / L; wherein the concentration of glucose in the feed solution is 400g / L, the concentration of soybean flour is 125g / L.

[0028] The fermentation medium used for fermentation is:...

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PUM

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Abstract

The invention provides a method for improving a nemadectin yield. The method utilizes Streptomyces cyaneogriseus BJX003 with the preservation number CGMCC No. 8731 as a producing strain to realize nemadectin fermentation production. When the broth has a reducing sugar concentration less than 12-18g / L, a glucose solution containing soybean cake powder is supplied and the reducing sugar concentration is controlled in a range of 12-18g / L. The supplement solution has a glucose concentration of 300-400g / L and a soybean cake powder concentration of 75-125g / L. The method can improve a nemadectin yield to about 3400 micrograms per milliliter from about 3200 micrograms per milliliter.

Description

technical field [0001] The present invention relates to the field of microbial fermentation, in particular to a method for improving the yield of nimoctin. Background technique [0002] Organic chemical pesticides were invented in the 1940s. Its appearance greatly enhanced the ability of human beings to control the damage of pests and diseases, and made a significant contribution to the recovery of crop yield losses. However, organic chemical pesticides generally have high toxicity, low safety, It has a great impact on the environment, and its long-term and extensive use has brought a series of well-known problems such as environmental pollution, ecological damage and food safety, which has seriously affected the sustainable development of agricultural economy. In the past ten years, countries have reduced the amount of chemical pesticides to varying degrees. In December 2003, the EU officially banned the sale of 320 kinds of pesticides in the EU, including more than 60 kind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/465
Inventor 王书睿周贤龙
Owner 牡丹江佰佳信生物科技有限公司
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