Solid ocean red yeast preparation as well as preparation method and application thereof
A marine red yeast, solid technology, applied in applications, animal feed, fungi, etc., can solve the problems of low cell density of solid marine red yeast, unfavorable long-term storage and long-distance transportation, and insufficient live bacteria rate, achieving bright color, The effect of increasing the live bacteria rate and cell density and increasing the growth rate
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Embodiment 1
[0041] The solid marine red yeast preparation in this example is mainly made of compound protective agent and concentrated marine red yeast bacterium slime, the mass ratio of the two is 1.5:1, and the cell density of the solid marine red yeast preparation is 3000~ 400 billion / g, the cell activity is 70~80%, the protective agent includes skimmed milk powder, sucrose and water, of which 5% skimmed milk powder (mass percentage, the same below), 10% sucrose (mass percentage, the same below) and the balance is water.
[0042] Wherein the concentrated marine rhodotorula spore is prepared by the following method:
[0043] (1) Test tube activation culture: under aseptic conditions, inoculate Rhodotorula japonicus strains on the slope of the test tube with sterilized seed culture solution, and activate the culture at 25°C for 48 hours;
[0044] Among them, the marine rhodotorula strain used in the present invention was isolated and preserved from seawater fish by the laboratory staff...
Embodiment 2
[0081] The solid marine red yeast preparation in this example is a powder, and it is mainly made of compound protective agent and concentrated marine red yeast fungus slime, the mass ratio of the two is 2.2:1, the cell density of the marine red yeast fungus powder 200-300 billion / g, cell viability 70-80%, protective agent includes skimmed milk powder, sucrose and water, skimmed milk powder 5% (mass percentage, the same below), sucrose 10% (mass percentage, the same below) same) and the balance is water.
[0082] Wherein the concentrated marine rhodotorula spore is prepared by the following method:
[0083] (1) Test tube activation culture: under aseptic conditions, inoculate Rhodotorula japonicus strains (same source as in Example 1) on a test tube slope with sterilized seed medium, and activate and cultivate at 28°C for 48 hours;
[0084] (2) Shake flask expansion culture: under aseptic conditions, use an inoculation loop to pick 2~3 rings of activated strains and inoculate...
Embodiment 3
[0099] The solid marine red yeast preparation in this embodiment is a powder, and it is mainly made of compound protective agent and concentrated marine red yeast fungus slime, the mass ratio of the two is 1.8:1, and the cell density of marine red yeast fungus powder is 300-400 billion / g, the cell activity is 70-80%, the protective agent includes skim milk powder, sucrose and water, skim milk powder 5% (mass percentage, the same below), sucrose 10% (mass percentage, the same below ), and the balance is water.
[0100] Wherein the concentrated marine rhodotorula spore is prepared by the following method:
[0101] (1) Activation and subculture: Rhodotorula colloides is used as the strain (from the intestine of wild shrimp, the isolation process is the same as in Example 1), the seed culture solution is used as the medium, the strain is activated and passed on a slant, and the culture temperature is 24~ 30°C, culture time is 36-48 hours;
[0102] (2) Expanded cultivation in sha...
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