39results about How to "High rate of live bacteria" patented technology

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention relates to a compound soil remediation agent containing microorganism bacteria and a preparation method thereof. The remediation agent comprises the following components in parts by weight: 0.01-3 parts of bacillus pasteurii spore powder, 5-50 parts of phosphogypsum powder, 10-60 parts of organic compost, 5-50 parts of turf powder, 1.5-5 parts of potassium fulvate powder, 0.2-0.5 part of powdery bacteria powder carriers and 0.15-1 part of glycerin. The remediation agent has round and smooth grains and high grain hardness, is harmless to seeds, can serve as a base fertilizer together with other fertilizers, can be sown together with seeds, integrates multiple remediation functions including organic complexation and passivation remediation, inorganic passivation remediation, microorganism passivation remediation and the like, has a comprehensive and strong soil remediation function, and can stimulate growth and reproduction of arbuscular mycorrhizal fungi at the roots of crops, accelerate heavy metal passivation, stimulate crop growth and enhance the immunities of crops.

The invention discloses Cryptococcus capsules and a method for preparing the same. The method for preparing the Cryptococcus capsules comprises the step of mixing a solution of alginate, Cryptococcus, multivalence metal cations to obtain the Cryptococcus capsules. Experiments show that the viable bacterium rates of the Cryptococcus capsules prepared by the method are all above 70 percent; the highest viable bacterium rate is up to 90 percent; and the activity of bacteria is not reduced. The Cryptococcus capsules of the invention can be stored at both low and normal temperature, the effect of storage for one month at normal temperature is substantially the same as that of the storage at low temperature and the biological activity of the bacteria are not influenced. Therefore, the method has wide application prospect in the preparation of biological agents and the preservation of other organisms.

The invention discloses a solid ocean red yeast preparation which is mainly prepared from a composite protective agent and ocean red yeast bacterial sludge according to a mass ratio of (1.5-2.2):1, wherein the cell density of ocean red yeast in the solid ocean red yeast preparation is 200-400 billion/gram, the cell activity is 70-80%, and the protective agent comprises skim milk powder and cane sugar. The invention further discloses a preparation method of the solid ocean red yeast preparation, The preparation method comprises the following steps: firstly, performing high-density cultivation on the ocean red yeast in a cultivation mode of continuous liquid flow layer ventilation, and further drying by using a vacuum freezing drying method. According to the solid ocean red yeast preparation, the survival rate of the ocean red yeast can be increased to be 70-80%, the cell density in the preparation can be 200-400 billion/gram, and the preparation can be also used in aquatic product feeds as an additive as a product of the preparation is in a solid manner, long in expiration date and convenient to transport.

The invention discloses a high-nutrient microbial bacterium composite fertilizer and a production method thereof. The high-nutrient microbial bacterium composite fertilizer comprises the following components in parts by weight: 16-190 parts of double-wall layer composite microbial bacterium microcapsules (containing 3-5 parts of composite microbial bacteria), 150-450 parts of monoammonium phosphate, 0-400 parts of urea powder, 100-200 parts of potassium chloride, 200-350 parts of ammonium chloride, 20-100 parts of ammonium bicarbonate, 0-150 parts of ammonium sulfate and 0-50 parts of attapulgite. The production method comprises the following steps: adsorbing the microbial bacteria by adopting germ powder thereof, performing first wall layer embedding by adopting a chemical cross-linking method, performing second wall layer embedding by adopting a spray drying process, and finally coating on the surface of chemical fertilizer granules, and split charging. The composite fertilizer is sufficient in nutrient and high in viable count, so that the microbial bacteria can be slowly released, yield increase of crops is guaranteed, the incidence rate of insect pests is reduced, and soil hardening caused by application of chemical fertilizers is improved.

The invention discloses a preparation method and application of high-stress-resistant probiotics. The preparation method comprises the steps that a porous material is placed in a culture medium, the culture medium is inoculated with probiotics, and the probiotics are cultured, wherein the culture medium contains growth promotion factors capable of promoting growth and propagation of the probiotics, and a natural polysaccharide or natural protein or synthetic macromolecule homopolymer or copolymer is adopted as the porous material; the probiotics gather on the surface of the porous material or in the porous material or on the surface of the porous material and in the porous material, and then the gather-state probiotics are obtained. According to the preparation method, the stress-resistant properties including the heat resistance, the acid and alkali resistance and the drug resistance of the probiotics can be significantly improved, the survival rate of the probiotics in application scenes such as a digestive system is increased, wide application of the probiotics in products such as food, health care products, medicines, feed, environmental treatment products, hygienic products and daily chemical products is promoted, and the activity of the probiotics in the product processing period and the shelf period can be effectively guaranteed.

The invention relates to probiotics microcapsule bacterial powder and a preparation method and application thereof. The probiotics microcapsule bacterial powder is prepared from core materials and wall materials, the core materials comprise probiotics, and the wall materials comprise polysialic acid. The polysialic acid creatively serves as the wall materials of microcapsules, the probiotics serves as the core materials of the microcapsules, thus the polysialic acid and the probiotics can exert respective physiological functions and can also synergistically act, a probiotics preparation can exert better properties, and the bacterial survival rate of the probiotics is remarkably increased; the activity of the probiotics can also be improved; and meanwhile the probiotics can be smoothly colonized in the intestinal tract, and the physiological effect is exerted better.

The invention relates to a freeze-drying protective agent for a listeria monocytogenes standard substance, a freeze-drying preserving method and application. The freeze-drying protective agent is a special freeze-drying protective agent for the listeria monocytogenes standard substance. Through the cooperation of the four specific components (skim milk, polyvinylpyrrolidone, cane sugar and tyrosine) according to the specific mass percentage contents, the synergistic interaction can be realized, the freeze-dried listeria monocytogenes standard substance reaches the high living bacterium rate, and the living bacterium rate of the standard substance can still be kept at a high level after the standard substance is stored for a long time.

The present invention discloses a probiotics sea buckthorn fruit puree jelly and a preparation method thereof, and belongs to the field of probiotics. A sea buckthorn fruit puree jelly is used as a carrier of probiotics; proteins, polysaccharides and other ingredients in sea buckthorn fruit puree are utilized; and at the same time, a gel substance is combined with thalli of bifidobacteria, lactobacillus, etc. to form the probiotics sea buckthorn fruit puree jelly. The probiotics sea buckthorn fruit puree jelly effectively reduces heat, oxidation and drying damages to the probiotics during a conventional drying process, achieves a purpose of long-term preservation of the probiotics, at the same time plays a role of resisting oxidation damages, effectively prevents vigor loss of bacterial strains during a storage process, also improves the additional nutritional value of the probiotics, and is widely used in food industry.

The invention relates to the technical field of health-care foods, in particular to a protein type health-care food composition and a preparation method thereof. Effective components of the composition consist of the following raw materials: poultry egg liquid, soybeans, collagen, cane sugar, linseed oil, honey, traditional Chinese medicine powder, sucrose fatty acid ester, xanthan gum, and microcapsule composite probiotics, wherein the traditional Chinese medicine powder is prepared from the following raw materials of radix codonopsis, haws, dried orange peel, sea-buckthorns and licorice roots. The protein is extracted from natural food materials, animal collagen is added, and the probiotics and traditional Chinese medicine components are effectively combined and jointly achieve the effect, so that the absorption and utilization rate of the protein is increased. A glue milling homogenizing technology is adopted, so that protein granules are changed into granules which are suitable forabsorption by human bodies, and digestion and absorption by the human bodies are facilitated. A secondary embedding technique is used, so that the activity of probiotics is reserved to the maximum extent, a secondary embedding wall material is modified starch resistant to high temperature, and the viable bacteria rate of the probiotics after UHT sterilization is greatly increased.

The invention provides orange velvet oat set yoghurt and a preparation method thereof. The orange velvet oat set yogurt is prepared from the following raw materials: raw milk, white granulated sugar,milk protein powder, low-fat milk powder, a compound stabilizer, jam and a direct vat set starter. According to the orange velvet oat set yoghurt, orange velvet oat pulp is added into the yoghourt forthe first time, a specific direct vat set starter is adopted, low-fat milk powder and protein powder are additionally utilized to increase the solid content and reduce the additive amount of a stabilizer to achieve the suspension characteristic, and the solidified yoghourt with uniformly distributed orange velvet oat jam with excellent sensory characteristics and texture characteristics is prepared.

The invention provides bacillus coagulans HQ-1 capable of generating plasmin. The bacillus coagulans HQ-1 is preserved in China general microbiological culture collection center, wherein the preservation number is CGMCC No.16058, and the classification designation is bacillus coagulans (Bacillus coagulans). The invention further provides a method for preparing the plasmin and viable bacteria tablets by using the bacillus coagulans HQ-1. The prepared bacillus coagulans has favorable drug sensitivity, bacterial inhibition and resistance to acid base environment. The obtained bacillus coagulans is high in plasmin activity, and can achieve 383.1U/mL. The obtained viable bacteria tablets are simple in preparation method, and high in viable bacteria rate, and have favorable practical applicationprospects.

The invention relates to active soy sauce dry yeast prepared from candida. The preparation method comprises the following steps: (1) activating candida by using a YPD liquid culture medium with the salt concentration of 2-2.2%, and carrying out enlarged culture and enrichment on the candida by using a double-concentration YPD culture medium with the salt concentration of 10-12%; (2) stimulating candida at 35 DEG C for 90min, recovering to 30 DEG C, culturing for 2-3h, and centrifugally collecting; and (3) carrying out vacuum freeze drying after centrifugal collection, and carrying out vacuum freeze drying at -80 DEG C for 20-22 hours by taking a trehalose aqueous solution with the mass concentration of 9% as a protective agent so as to obtain the active soy sauce dry yeast. According to the method, the active dry yeast is high in viable organism rate and good in salt tolerance, and when the active dry yeast is applied to fermentation of high-salt liquid-state soy sauce and solid-liquidcombined soy sauce, compared with soy sauce added with fresh yeast, indexes such as amino acid nitrogen and reducing sugar of a soy sauce finished product are improved.