39results about How to "High rate of live bacteria" patented technology

The invention discloses a broad-bean chili sauce maturity-promotion and aroma-enhancement fermentation bacterium agent and a preparation method thereof. The preparation method comprises the following steps: performing propagation culture on bacillus pumilus, lactobacillus fermentum, lactobacillus bifermentans, saccharomyces rouxii and aspergillus oryzae; mixing, inoculating to a sterilization matrix carrier culture medium by 4%-8%, and culturing at 32 DEG C for 48 hours, wherein the colony count is 108-1,010cfu / mL; after the fermentation is finished, adding 6%-10% of sucrose, 1%-5% of gelatin and 2%-5% of glycerin as heat-resistant protectants, flatly laying on a thin layer, performing far-infrared drying at 50-60 DEG C for 2-3 hours, and packaging in vacuum. According to the fermentation bacterium agent, the maturity promotion, aroma enhancement and fermentation of broad-bean chili sauce are enhanced and the quality of the broad-bean chili sauce is stabilized and improved.

The invention discloses a probiotic ultralow temperature refrigeration technology, a formula of a total-salt cryoprotectant which is suitable for the probiotic ultralow temperature refrigeration technology, and probiotic ultralow temperature refrigeration particles prepared by using the probiotic ultralow temperature refrigeration technology. The probiotic ultralow temperature refrigeration technology comprises following steps: (1) preparing a fermentation medium; (2) culturing probiotics; (3) centrifuging; (4) preparing the cryoprotectant; (5) adding the cryoprotectant; and (6) quick-freezing and moulding. The invention also provides a probiotic preparation prepared by using the probiotic ultralow temperature refrigeration particles, and a preparation method of the probiotic preparation. The probiotic ultralow temperature refrigeration particles are probiotic refrigerated bacterial strains which do not need to be dried and possesses advantages of fast refrigeration speed, high living bacterial rate and excellent rehydration performance. The probiotic ultralow temperature refrigeration particles contain water, can be dissolved in solutions more quickly, and are capable of preventing probiotic cells from damage caused by rehydration of normal low temperature refrigeration dried probiotic powder, and increasing the living bacterial rate of the probiotic preparation greatly.

The present invention relates to a gene encoding trehalose-6-phosphate phosphatase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and / or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and / or resistance property to low-temperature storage is enhanced by amplifying expression level of TPS2 gene encoding a trehalose-6-phosphate phosphatase Tps2p in brewer's yeast, especially non-ScTPS2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.

The invention relates to a method for embedding lactic acid bacteria by using Maillard products as wall materials, and belongs to the technical field of protection of lactic acid bacteria. The method is to fully dissolve the protein and carbohydrates, react at 60-90°C for 30-60min, make the mixed solution appear yellowish brown, then disperse the lactic acid bacteria concentrate in the mixed solution, and spray dry to obtain lactic acid bacteria with high stability Good bacteria powder. The protein is selected from casein, whey protein, soybean protein, egg white protein or protein hydrolyzate, and the carbohydrate is composed of reducing sugar and prebiotic sugar in equal weight ratio. The powder obtained by the invention can effectively improve the survival rate of lactic acid bacteria in the spray drying process, storage period and human digestive tract, and is easy to implement and low in cost.

The invention discloses a lactic acid bacterium or yeast porous starch embedding and protecting method. Lactic acid bacteria or yeasts are cultured in a fermentation tank with high density to make the total amount of the lactic acid bacteria in a fermentation liquor reach 3.4 to 4.5 * 10<9>CFU / mL and the total amount of the yeasts reach 2 to 3 * 10<10>CFU / mL; the fermentation liquor is concentrated by 15 to 20 times in vacuum to obtain a concentrated bacterial liquid; porous starch, dried whey and trehalose are added into the concentrated bacterial liquid; and after being uniformly stirred until the mixture is pasty, the mixture is dried to obtain a product with relatively high viable bacteria content. The amount of the viable bacteria in the lactic acid bacterium or yeast embedding preparation produced by the technique is over 10<10>CFU / g; the product is resistant in storage; the production process is simple; and the cost is low.

The present invention relates to a gene encoding glycogen synthase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and / or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and / or resistance property to low-temperature storage is enhanced by amplifying expression level of GSY1 or GSY2 gene encoding a Gsy1p or Gsy2p which is a glycogen synthase in brewer's yeast, especially non-ScGSY1 gene or non-ScGSY2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.

The invention relates to a compound soil remediation agent containing microorganism bacteria and a preparation method thereof. The remediation agent comprises the following components in parts by weight: 0.01-3 parts of bacillus pasteurii spore powder, 5-50 parts of phosphogypsum powder, 10-60 parts of organic compost, 5-50 parts of turf powder, 1.5-5 parts of potassium fulvate powder, 0.2-0.5 part of powdery bacteria powder carriers and 0.15-1 part of glycerin. The remediation agent has round and smooth grains and high grain hardness, is harmless to seeds, can serve as a base fertilizer together with other fertilizers, can be sown together with seeds, integrates multiple remediation functions including organic complexation and passivation remediation, inorganic passivation remediation, microorganism passivation remediation and the like, has a comprehensive and strong soil remediation function, and can stimulate growth and reproduction of arbuscular mycorrhizal fungi at the roots of crops, accelerate heavy metal passivation, stimulate crop growth and enhance the immunities of crops.

The invention discloses a freezing and drying protecting agent for an animal derived gelatin-free vaccine. The vaccine freezing and drying protecting agent takes sterile water for injection as a solvent and each litre of the freezing and drying protecting agent comprises 20-100g of saccharose, 5-20g of sodium glutamate and 5-20g of thiourea. The freezing and drying protecting agent is applied to the freezing and drying protection of a brucella live vaccine for amyxis.

The present invention provides a composite micro-organism agent and a freeze-dried micro-organism agent for fermenting apple pomace, and an apple pomace protein feed, and relates to the technical field of animal fermented feeds. The composite micro-organism agent for fermenting the apple pomace comprises the following components in parts by volume: 29-36 parts of geotrichum candidum, 16-36 parts of yeasts, 8-31 parts of bacillus subtilis, 8-17 parts of rhizopus and 8-17 parts of trichoderma viride; and effective viable micro-organism number of the geotrichum candidum, yeasts, bacillus subtilis, rhizopus and trichoderma viride are respectively independently larger than or equal to 1x10<9> cfu / ml. The composite micro-organism agent can compensate defects for each other during fermentation and is subjected to synergistic mutual fermentation. The apple pomace protein feed produced by the provided composite micro-organism agent has a crude protein content of 20% or more, the total of 16 amino acids is more than 7.54 g / 100 g, and the composite micro-organism agent is low in fiber, comprehensive in nutrition, green, safe, low in cost and high in benefits.

The invention discloses a vaccine freezing-drying protecting agent without animal origin gelatin components. The agent takes sterile injection water as a solvent, and each liter of the protecting agent contains 10-100g of lactose, 5-20g of sodium glutamate and 5-20g of thiourea. The vaccine freezing-drying protecting agent is applied to freezing-drying protection on brucellin live vaccines for intradermal injection.

The invention discloses a high-nutrient microbial bacterium composite fertilizer and a production method thereof. The high-nutrient microbial bacterium composite fertilizer comprises the following components in parts by weight: 16-190 parts of double-wall layer composite microbial bacterium microcapsules (containing 3-5 parts of composite microbial bacteria), 150-450 parts of monoammonium phosphate, 0-400 parts of urea powder, 100-200 parts of potassium chloride, 200-350 parts of ammonium chloride, 20-100 parts of ammonium bicarbonate, 0-150 parts of ammonium sulfate and 0-50 parts of attapulgite. The production method comprises the following steps: adsorbing the microbial bacteria by adopting germ powder thereof, performing first wall layer embedding by adopting a chemical cross-linking method, performing second wall layer embedding by adopting a spray drying process, and finally coating on the surface of chemical fertilizer granules, and split charging. The composite fertilizer is sufficient in nutrient and high in viable count, so that the microbial bacteria can be slowly released, yield increase of crops is guaranteed, the incidence rate of insect pests is reduced, and soil hardening caused by application of chemical fertilizers is improved.

The present invention relates to a gene encoding a protein having a trehalose synthesis-promoting activity and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and / or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and / or resistance property to low-temperature storage is enhanced by amplifying expression level of TSL1 gene encoding a protein Tsl1p having a trehalose synthesis-promoting activity in brewer's yeast, especially non-ScTSL1 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.