The invention discloses a micromolecular
ovalbumin peptide with effects of improving exercise tolerance and relieving
oxidative stress and a preparation method of the micromolecular
ovalbumin peptide, and belongs to the technical field of
functional protein peptide preparation. The preparation method of the micromolecular
ovalbumin peptide comprises the following steps: taking
egg white protein, carrying out heat treatment and
centrifugation, collecting supernate, adding a compound
enzyme preparation for enzymolysis, carrying out plate frame
filtration, adjusting the pH value to 5.5-7.0 and the
salt content to be less than or equal to 0.1 mol L <-1 >, loading and adsorbing by using non-polar
macroporous resin, washing by using pure water which is 1-2 times of the volume of the resin after the loading is finished, and
drying to obtain the micromolecular ovalbumin peptide. Eluting by using a 10%-30%
ethanol solution, and collecting an eluent; and performing concentration,
solvent removal,
desalination and
drying on the eluent by using a
nanofiltration unit to obtain the micromolecular ovalbumin peptide. The
small molecule peptide has high
antioxidant activity, and can realize the triple effects of immediate absorption, rapid antioxidation and synchronous anti-
inflammation; and the preparation process is simple, and industrial
mass production can be realized.