A method for embedding lactic acid bacteria using Maillard products as wall material
A technology of lactic acid bacteria and rad, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as insufficient protection effect, and achieve the effects of easy implementation, increased viable bacteria rate, and improved survival rate
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Embodiment 1
[0030] Add 500g of whey protein into 3000mL sterile water, stir at 60°C at 70r / min for 45min, after it is completely dissolved, add 250g of glucose and 250g of resistant starch, totaling 500g, in order to effectively mix the resistant starch, the The mixture needs to be homogenized twice with a homogenizer at 250bar, and left to degas for 45 minutes; the above protein + carbohydrate solution is subjected to Maillard reaction at 85°C for 40 minutes, and the reaction is terminated immediately when the solution turns yellowish brown. And use condensed water to quickly cool it to room temperature; then 32g of Lactobacillus plantarum sludge (viable bacteria number 5.5~7.0×10 11 CFU min / g) was added to the mixed solution, stirred at 80r / min for 40min to disperse evenly, and left to degas for 45min; finally, the obtained mixed bacterial solution was sprayed under the conditions of hot air inlet temperature 120°C and outlet air temperature 55°C Dried (the moisture content of the prod...
Embodiment 2
[0032] Add 400g soybean protein into 3000mL sterile water, stir at 60°C at 90r / min for 60min, after it is completely dissolved, add 500g lactose + xylooligosaccharide (each 50%), stir until completely dissolved, then statically Degassed for 60 minutes; the above protein + carbohydrate solution was subjected to Maillard reaction at 80 °C for 60 minutes, and when the solution was yellowish brown, the reaction was immediately terminated, and it was rapidly cooled to room temperature with condensed water, and then 35 g of Lactobacillus casei Mushroom slime (the number of live bacteria is 2.5~5.0×10 11 CFUmin / g) into the mixed solution, stirred at 60r / min for 60min until uniformly dispersed, and left to degas for 50min; finally, the obtained mixed bacterial solution was spray-dried under the conditions of hot air inlet temperature 120°C and outlet air temperature 55°C (moisture content of the product is 4.6%) to obtain the bacteria powder, the content of Lactobacillus casei in the b...
Embodiment 3
[0034] Add 450g of casein into 3000mL sterile water, stir at 60°C for 50min at 60r / min, after it is completely dissolved, add 500g of maltotriose + fructooligosaccharide (each 50%), stir until completely dissolved, Stand for degassing for 30 minutes; carry out the Maillard reaction of the above protein + carbohydrate solution at 90°C for 30 minutes, stop the reaction immediately when the solution turns yellowish brown, and quickly cool it to room temperature with condensed water, and then add 33g of acidophilus Lactobacillus sludge (viable bacteria count 2.5~5.0×10 11 CFU min / g) was added to the mixed solution, stirred at 90r / min for 30min to disperse evenly, and left to degas for 60min; finally, the obtained mixed bacterial solution was sprayed under the conditions of hot air inlet temperature 140°C and outlet air temperature 70°C Drying (product moisture content 4.0%), obtain bacterial powder, the content of Lactobacillus acidophilus in bacterial powder is 1.2×10 10 CFU / g....
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