Preparation method and application of high-stress-resistant probiotics

A technology of probiotics and high resistance, applied in the direction of bacteria, fungi, etc., can solve the problems of limited activity of probiotics, high additional cost, cumbersome process, etc., to achieve increased growth and reproduction rate, improved stress resistance, and increased live bacteria rate Effect

Inactive Publication Date: 2017-02-22
杭州益儒信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such methods are cumbersome and costly, and are rarely used in actual production, and the activity of the embedded probiotics is still limited, which cannot meet the needs of actual industrial processing and long-term storage at room temperature shelves, and is resistant to adverse environmental factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] First add cellulose / chitosan gel to the culture medium, the porosity of the gel is 90%, and the average pore size is 500nm. The weight percentage of each component in the culture medium is 75% of MRS, 1% of L-cysteine ​​hydrochloride, 1% of vitamin C, 5% of carrot juice, 10% of shiitake mushroom extract and 8% of soybean milk. Bifidobacteria were inoculated into the medium, and the bifidobacteria were purchased from public channels of the China Industrial Microbiology Culture Collection and Management Center, and the concentration of bifidobacteria was 10 9 CFU / mL of bacterial liquid, the amount of inoculated bacterial liquid is 20%. Anaerobic culture, after 16 hours of culture at 37°C, the gel particles of bifidobacterium aggregation were obtained.

[0042] The bifidobacteria have good stress resistance. After being treated at 200°C for 10 minutes, 88% of the bifidobacteria retain their activity; when they are stored at room temperature for 6 months, 92% of the bifido...

Embodiment 2

[0044] Firstly, a collagen / trehalose porous membrane is added to the culture medium, the porosity of the porous membrane is 80%, and the average pore size is 200nm. The weight percentage of each component in the culture medium is 80% of MRS, 4% of sucrose, 1% of magnesium salt, 5% of corn juice, 5% of shiitake mushroom extract and 5% of whey fermented liquid. Then Lactobacillus delbrueckii Lactobacillus bulgaricus was inoculated into the medium, and the concentration of Lactobacillus delbrueckii Lactobacillus bulgaricus was 10 9 The CFU / mL bacterial liquid, Lactobacillus delbrueckii Lactobacillus bulgaricus was purchased from the public channel of China Industrial Microbiology Culture Collection and Management Center. Anaerobic culture, after 12 hours of culture at 37 ° C, Lactobacillus delbrueckii Lactobacillus bulgaricus aggregated on the surface of the porous membrane.

[0045] The Lactobacillus delbrueckii Lactobacillus bulgaricus has good stress resistance. After being t...

Embodiment 3

[0047] The fibroin fiber material is added into the culture medium, the fibroin fiber material is 82%, and the average pore size is 400 μm. The weight percentage of each component in the culture medium is 85% of YPD, 4% of malt extract, 1% of vitamin, 5% of oyster mushroom extract, 2% of ganoderma lucidum extract and 3% of collagen fermented liquid. Then the yeast was inoculated into the medium, and the yeast was used at a concentration of 10 8 CFU / mL bacterial liquid, yeast was purchased from public channels of China Industrial Microbiology Culture Collection and Management Center. Anaerobic culture, after 24 hours of culture at 30°C, a large amount of yeast accumulated on the surface and inside of the foam.

[0048] The yeast product has good stress resistance. After being treated at 120°C for 10 minutes, 93% of the yeast retained its activity; after being stored at room temperature for 6 months, 96% of the yeast retained its activity; Bacteria retain activity; after treat...

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PUM

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Abstract

The invention discloses a preparation method and application of high-stress-resistant probiotics. The preparation method comprises the steps that a porous material is placed in a culture medium, the culture medium is inoculated with probiotics, and the probiotics are cultured, wherein the culture medium contains growth promotion factors capable of promoting growth and propagation of the probiotics, and a natural polysaccharide or natural protein or synthetic macromolecule homopolymer or copolymer is adopted as the porous material; the probiotics gather on the surface of the porous material or in the porous material or on the surface of the porous material and in the porous material, and then the gather-state probiotics are obtained. According to the preparation method, the stress-resistant properties including the heat resistance, the acid and alkali resistance and the drug resistance of the probiotics can be significantly improved, the survival rate of the probiotics in application scenes such as a digestive system is increased, wide application of the probiotics in products such as food, health care products, medicines, feed, environmental treatment products, hygienic products and daily chemical products is promoted, and the activity of the probiotics in the product processing period and the shelf period can be effectively guaranteed.

Description

technical field [0001] The present invention relates to a preparation method and application of bacteria, in particular to a preparation method and application of highly stress-resistant probiotics. Background technique [0002] Probiotics, the word probiotics in English is derived from the Greek word "for live", translated as "for life". It can colonize the host and improve the micro-ecological balance of the host, thereby playing a beneficial role. Studies in recent years have shown that there are more than 1,000 kinds of bacteria in the human body, especially the bacteria in the gastrointestinal tract are the most abundant, accounting for about 80% of the total number of microecological bacteria in the whole body, which is 10 times that of human cells. Two Genomes". These bacteria help the body digest food, produce vitamins to prevent diseases caused by bacteria in food, and stimulate the immune system. With the deepening of research, it has been found that gut microbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14
CPCC12N1/20C12N1/14C12N1/16
Inventor 胡梦欣王晓剑
Owner 杭州益儒信息科技有限公司
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