The invention belongs to the technical field of
mushroom deep processing, and discloses a biological enzymolysis
mushroom extract and a preparation method thereof. The preparation method comprises thefollowing steps that raw materials are pretreated,
enzyme deactivation is carried out, and after enzymolysis is carried out, cooling is carried out to reach the normal temperature so as to obtain
hydrolysate; secondary enzymolysis is carried out, specifically, 5'-
phosphodiesterase is added, the pH value is adjusted, heating is carried out to reach 50-60 DEG C, reaction is carried out for 1.5-2.5hours, heating is carried out to reach 95-100 DEG C, the temperature is kept for 10-20 minutes,
enzyme deactivation is carried out, after enzymolysis is carried out, cooling is carried out to reach the normal temperature, filtering is carried out through a 60 to 80-mesh screen or
centrifugal separation is carried out so as to obtain enzymatic
hydrolysate; blending is carried out, specifically, a multi-
enzyme enzymolysis
mushroom extracting solution, beta-cyclodextrine and edible salt are uniformly mixed; and
drying is carried out, specifically,
spray drying is carried out on the
mixed materials so as to obtain the mushroom extract. According to the mushroom extract and the preparation method thereof, a multi-enzyme enzymolysis technology is adopted to optimize extraction of mushroom flavouring materials, through multi-enzyme enzymolysis, the time can be saved, the
energy consumption can be reduced, and the production efficiency can be improved; and the staged enzymolysis conditions aremild and are easy to control, and the obtained enzymatic
hydrolysate contains rich
amino acid,
nucleotide,
lentinan and other active ingredients.