58 results about "Enzymic hydrolysis" patented technology

PCT No. PCT/EP96/05025 Sec. 371 Date May 26, 1998 Sec. 102(e) Date May 26, 1998 PCT Filed Nov. 12, 1996 PCT Pub. No. WO97/19601 PCT Pub. Date Jun. 5, 1997Fish oil concentrates, having: </=40% of long chain poly unsaturated fatty acids <20% of saturated fatty acids (C14-C18) <15% oleic acid <12% C16:1-fatty acid and with a weight-ratio: can be obtained by a process involving the following steps subjecting a fish oil to an enzymic conversion removing free fatty acids or esters from conversion-product subjecting product of above to enzymic hydrolysis, using specific lipase or partial glyceride-specific lipase wash and dry product re-esterify dried product.

The invention relates to a preparation method of epimedium aglycone, which comprises the steps that dried epimedium is pulverized or cut into segments, 30-95% ethanol or 30-100% methanol is added for reflux extraction, the extract solution is decompressed and concentrated to obtain paste, 10-100 ml of acetate-sodium acetate buffer solution with the pH of 3.5-9.0 is added into each gram of paste, snailase is added for hydrolysis, the mass ratio of enzyme to paste is 0.05-5, the reaction temperature is 15 to 80 DEG C, the reaction time is 2 to 72h, the reaction solution is centrifuged, the precipitate is dissolved with an organic solvent, filtration is carried out to obtain filtrate, the solvent is steamed away to obtain crude epimedium aglycone, and pure epimedium aglycone is obtained after recrystallization. The invention has the characteristic that the epimedium extract is directly converted into high-purity aglycone in one step by utilizing the strong enzymic hydrolysis of the snailase so as to simplify the operation steps with strong purposiveness and high conversion rate, and has the advantages of no pollution and large-scale production. The invention overcomes the shortcomings of large destruction on the aglycone, serious pollution, poor purposiveness, low conversion rate, difficult separation and purification and the like of the prior art.

The invention relates to a lactoferrin antibacterial peptide, the preparation method and the application thereof. The method utilizes the lactoferrin and the enzyme preparation in bovine colostrum to prepare the lactoferrin antibacterial peptide zymolyte through enzymic hydrolysis, and subjects the zymolyte to macroporous absorption static or dynamic desalination, gel filtration chromatography, half-preparation high efficiency liquid chromatography and analysis-type high efficiency liquid chromatography for separation and purification to obtain a pure lactoferrin antibacterial peptide product. The product has high-efficient and broad-spectrum antibacterial capability, thereby having broad prospects for application in food, feed and medical fields.

The invention relates to a method for preparing brain protein hydrolysate for injection and a preparation thereof. The brain protein hydrolysate is prepared from pig brain tissues passing quarantine through a specific controlled enzyme hydrolysis technique, and active ingredients of the prepared brain protein hydrolysate comprise polypeptide with a molecular weight of less than 10,000 Daltons and 16 free amino acids which are hydrolyzed amino acids; each milligram of total nitrogen of the obtained brain protein hydrolysate contains 4.68 to 7.02 milligrams of amino acids; the preparation method comprises the processes of homogenization, primary enzymolysis, inactivation, secondary enzymolysis, cation exchange column adsorption, desorption, blending, ultra-filtration, preparation and the like; when the obtained brain protein hydrolysate is used for preparing the preparation, an amino acid does not need adding; and pharmacological experiment results show that the prepared preparation has good treatment effect and good safety.

The present invention discloses a method for preparing astragalus methylglucoside by utilizing enzymatic hydrolysis of astragalus saponin glycosyl. It is characterized by that it uses an enzyme method to hydrolyze astragalus saponin glycosyl to prepare astragalus methylglucoside. The described enzyme is prepared by using astragalus saponin or flavone or isoflavone as fermentation enzyme-producing inductor and utilizing bacteria, streptomycete, mold, saccharomycetes and basidiomycetes to make fermentation. Said invention can be used for preparing astragalus tablet and powder products with high astragalus methylglucoside content.

The invention concerns concentrates of shea sterols in glycerides with more than 12.5 wt % shea sterols, the preparation thereof by enzymic hydrolysis of glycerides in shea oils or fractions thereof and the application of the concentrates in aerated food products.

The invention provides green tea goat milk with a function of reducing blood pressure and a preparation method of the green tea goat milk. Goat milk beverage which is rich in ACE inhibitory peptides is prepared by compound enzymolysis, is low in production cost, not limited by resource and small in environmental pollution, and can be mass-produced, and products are safe. On the basis, ultra-fine green tea powder is prepared by enzymic hydrolysis of cell walls and a low-temperature ultra-fine pulverizing technology, specific surface area is increased, and a large number of effective constituents are released. The ultra-fine green tea powder is added in the goat milk in proportion, and the obtained product not only has various nutrient substances and tea fragrance in green tea, but also has treatment effect of reducing high blood pressure, and has wide application prospect in the food industry. The tea powder is prepared by low-temperature ultra-fine pulverization, the original color, flavor and taste quality of tea leaves can be maintained effectively, after the tea leaves is pulverized into ultra-fine powder, the specific surface area of the tea powder is increased obviously, the effective constituents are released easily, human absorption can be improved, and therefore, nutrition absorptivity of human to the tea leaves is improved. Ultra-fine pulverization is a pure physical process, and pure natural property of processed products is maintained.

An extruded foodstuff having a protein content in the range of 45% to 80% protein by mass and specific density of 0.10-0.40 g/cm³, including: a vegetable protein isolate which has been at least partly hydrolysed by enzymic hydrolysis, which provides the majority of the protein content and which has a relatively low level of low molecular weight peptides and which has a relatively low water imbibing capacity; up to 30% by mass of wheat gluten; up to 5% by mass of the foodstuff is sodium bicarbonate.

The invention provides a method for detecting estrogens and estrogen metabolites in human urine, and relates to the technical field of estrogen and estrogen metabolite detection. According to the detection method, the human urine is mixed with an internal standard solution, and then a mixed solution is subjected to enzyme hydrolysis, organic solvent extraction, reversed phase medium solid-phase extraction purification and target product chemical derivatization in sequence. The full dissolution of the estrogens and the estrogen metabolites in the urine is guaranteed, and the interference of impurities in the urine is eliminated, so that the accuracy and stability of a detection result are ensured. Then, a liquid chromatography separation and mass spectrometry ion pair detection combined method is adopted for carrying out synchronous qualitative and quantitative analysis on the estrogens and the estrogen metabolites. The method is applicable to the synchronous batch detection of the estrogens and the estrogen metabolites with similar structures and chemical physical properties in the human urine, and has the advantages of high sensitivity, high precision, good repeatability, high detection speed and the like. The method fully meets the requirements of clinical examination, and has relatively high clinical application values.

The enzymic hydrolysis process of icariin glycosylate to prepare low sugar icariin or aglycone includes the following steps: taking enzyme capable of hydrolyzing icariin glycosylate; mixing the enzyme capable of hydrolyzing icariin glycosylate, icariin and buffering liquid for reaction for 1-36 hr at temperature 4-75 deg.c and pH 3-9; and extracting low sugar icariin or aglycone. The said process has the advantages of no pollution, high low sugar icariin or aglycone converting rate and less damage to effective component.

The present invention relates to a method for preparing hydrolyzed phospholipids by utilizing ultrasonic treatment and enzymic hydrolysis modification, belonging to the field of phospholipids modification technology. Said invention uses soybean hydrous oil foot as raw material, uses the phosphatidase A2 as enzyme for enzymic hydrolysis and adopts ultrasonic treatment method to make the soybean hydrous oil foot be modified in water phase so as to prepare the hydrolyzed phospholipids.

The present invention relates to a process for obtaining fuel ethanol by using agricultural and agroindustrial waste materials composed of lignocellulose, and especially sugar cane bagasse. These residues have significant contents of carbohydrates in the form of polysaccharides (cellulose and hemicellulose), which can be hydrolysed by chemical and enzymic processes. The hemicellulose fraction is submitted to mild hydrolysis with sulphuric acid, and the solid material from this hydrolysis is submitted to a process of saccharification (enzymic hydrolysis) with simultaneous rapid alcoholic fermentation under conditions which allow a significant increase in conversion to alcohol in a greatly shortened time.

The invention relates to composite seafood seasoning, and belongs to the technical field of seasoning. The composite seafood seasoning comprises enzymatic hydrolysate of clam juice, enzymatic hydrolysate of oyster juice, enzymatic hydrolysate of scallop skirts, and a shiitake mushroom extract, wherein the enzymic hydrolysis conditions of the clam juice, the oyster juice and the scallop skirts include that the temperature is 45-46 DEG C, the mass percentage of enzymes is 1%, the pH is 6.5-7.5, the enzymic hydrolysis time is more than 3h, and required enzymes are one or more of papain, neutral protease and flavored protease. The seasoning disclosed by the invention is delicious in taste, and has the peculiar fragrance of seafood; in addition, the preparation technology is simple and convenient, so that the seasoning has wider application and development prospects.

To provide a method for producing a seasoning liquid that is an improvement in a method for high-temperature salt-free brewing of a soy sauce Koji with which the hydrolytic time of unrefined soy sauce with a Koji mold enzyme is shortened and the nitrogen utilization ratio is remarkably increased to obtain the seasoning liquid without or scarcely having the aroma unique to the resultant soy sauce and without an off-taste and an off-odor for about 20 days while especially preventing the deterioration of the taste of the unrefined soy sauce obtained by mixing the soy sauce Koji with water (or a saline solution). The method for producing the seasoning liquid comprises mixingthe soy sauce Koji with hot water at 70-80[deg.]C, preparing the unrefined soy sauce at 50-57[deg.]C material temperature, intermittently or continuously stirring the unrefined soy sauce in the presence of common salt at 0-5 (wt./vol.)% concentration while maintaining the material temperature and carrying out enzymic hydrolysis for 15-30 h. (C)2004,JPO.

The invention relates to the technical field of immunoassay, in particular to a kit for preparing a CA125 surface Tn antigen by magnetic particle chemiluminescence immunoassay and a preparation methodthereof. The kit comprises a magnetic separation reagent coupled with a Fab fragment of a CA125 antibody, a biotin-lectin reagent, and a streptavidin-biotin-alkaline phosphatase coupling reagent, wherein the biotin-lectin reagent containing sialidase is capable of identifying the Tn antigen. The magnetic separation reagent is specifically bound to the CA125 antigen; the biotin-lectin specificallyrecognizes the Tn antigen of the CA125 antigen surface; the streptavidin-biotin-alkaline phosphatase coupling reagent is specifically bound to the biotin-lectin reagent to realize amplification of the detection signal; the sialidase hydrolyzes a glucosidic bond connected with the sialic acid on the Tn antigen surface and the sialic acid is released to expose the Tn antigen. Therefore, the detection accuracy, sensitivity and linear range are improved. The kit has advantages of high specificity and high sensitivity and is capable of realizing fully automated testing.

The invention provides an anti-tumor targeted drug sustained-release carrier, a preparation and a preparation method thereof. The preparation method comprises the following steps: S1, preparing a polyphosphoester and amino acid copolymer; S2, preparing a polylactic acid and glycolic acid copolymer solution; S3, preparing an amphiphilic compound; S4, preparing an amphiphilic compound connected witha targeting molecule; and S5, preparing the nano microspheres. The drug selectivity and positioning accuracy are greatly improved through the anti-tumor targeted drug sustained-release carrier, antitumor drugs are released after enzyme hydrolysis, cancer cells are efficiently killed, the drug effect is improved, the sustained release preparation can effectively avoid the blood concentration peakvalue after drug taking, the blood concentration is controlled to be in a stable curve, and the drug effect durability is improved.

The present invention relates to a method for extracting diosgenin from Japanese yam by means of enzymic hydrolysis and supercritical CO2 fluid extraction process. It is characterized by that firstly, it uses cellulose or alpha-diastase to hydrolyze Japanese yam raw material, then using CO2 fluid to make supercritical extraction for extracting diosgenin from Japanese yam.

The invention discloses a lipase mutant deriving from talaromyces thermophilus, a coding gene and application thereof in hydrolysis of racemic 2-carboxyethyl-3-cyano-5-methyl ethyl hexanoate to synthesize (3S)-2-carboxyethyl-3-cyano-5-methyl hexanoate. The mutant is obtained by mutation of one or more 206, 207 and 259 amino acids of an amino acid sequence shown in SEQ ID No.1. By means of protein molecule modification, the activity of TTL stereoselectivity CNDE hydrolysis is improved from 4.50U/mg to 160.55U/mg, and is much higher than the activity of lipase CNDE hydrolysis reported currently. Therefore, the mutant obtained in the invention has a great application prospect in high-efficient CNDE catalysis to synthesize pregabalin chiral intermediate (3S)-2-carboxyethyl-3-cyano-5-methyl hexanoate.

The invention provides a PLK1 inhibitor, and a preparation method and an application thereof. The PLK1 inhibitor is a compound represented by general formula (I) shown in the description, and stereoisomers or pharmaceutically acceptable salts thereof. All groups in the formula (I) are defined as in the description. The PLK1 inhibitor can specifically bind to a DNA sequence in a PLK1 gene transcription promoter region, represented by SEQ: NO:1, in a high strength manner, inhibits transcription of the PLK1 gene, inhibits the expression of a PLK1 protein, causes tumor cell growth inhibition or apoptosis, can traverse through a cell membrane and a nuclear membrane and resist nuclease hydrolysis, and also solves the drug resistance problem of micro-molecular kinases inhibitors.

The invention relates to a preparation method of a metal-matrix self-lubricating material and belongs to the field of preparation of self-lubricating materials. The method comprises the following steps of performing compound enzymic hydrolysis on an eel rich in amino acid and a pineapple to extract enzymatic hydrolysate; performing surface modification on graphite powder by using the enzymatic hydrolysate to improve the hydrophilicity of the graphite powder; then coating the surface of the graphite powder with a layer of metal palladium to realize effects of catalyzing and crystallizing a nucleation center; finally, chemically plating a layer of copper, thus obtaining the metal-matrix self-lubricating material. According to the preparation method disclosed by the invention, by performing surface modification on graphite, an interface structure and the wettability between a matrix material and the graphite are improved, interface bonding between the matrix and the graphite is enhanced, and comprehensive mechanical properties and tribological properties of a copper-matrix graphite self-lubricating composite material are favorably improved; the problems that the wettability of the graphite and the metal matrix is poorer, the interface bonding strength is low, stress concentration is easily caused, and the mechanical and properties and the tribological properties of the composite material are weakened are solved; the preparation method has a broad application prospect.

The invention relates to the technical field of DNA self-assembled membranes, particularly to a method for quantitative evaluating the HP DNA hairpin configuration on a substrate surface based on enzymic hydrolysis ability and a background signal eliminating method based on enzymic hydrolysis ability. The specific steps comprise: removing dimers through high temperature denaturation; immersing a substrate in an exonuclease I buffer solution having an enzyme amount of 100-300 U, and carrying out hydrolysis; and determining the proportion of the HP DNA hairpin configuration on the surface of anelectrode by using an electricity integration technology. According to the present invention, exonuclease has difference between in hydrolysis of ssDNA and in hydrolysis of HP DNA hairpin configuration, and the electrochemical method is combined to quantitatively evaluate the proportion of the HP DNA hairpin configuration on the surface of the substrate; and with the exonuclease I, the backgroundsignal caused by non-hairpin configuration can be effectively eliminated so as to substantially improve the detection sensitivity of the Hairpin DNA-based biosensor.

The invention provides a high-absorptivity compound protein composition as well as a preparation method and application thereof, and belongs to the technical field of protein. The preparation method comprises the following steps: adding whey protein powder into a disodium hydrogen phosphate-citric acid buffer solution, inoculating lactobacillus plantarum, bacillus coagulans and bifidobacterium adolescentis for fermentation, adding compound protease into fermentation liquor for enzymolysis to obtain enzyme hydrolysate, further performing synchronous ultrasonic wave and ozone treatment, performing enzymolysis through cross-linked compound enzyme, and performing freeze-drying to obtain the whey protein powder. And adding a metal ion solution, heating, stirring and reacting to prepare the high-absorptivity composite protein composition. The high-absorptivity composite protein composition prepared by the invention is moderate in molecular weight, does not cause a first-pass effect of liver, has high absorption and utilization rate, is rich in protein function, and has obvious effects of resisting aging, resisting oxidation, promoting fat burning and promoting metabolism.

The invention relates to the technical field of measuring and testing and discloses an enzymological detection method for detecting concentrations of citrulline, asymmetric dimethylarginine and the like in a reaction system by ATP (adenosine triphosphate) detection. According to the basic principle of reaction, ADMA (asymmetric dimethylarginine) is hydrolyzed by dimethylarginine dimethylaminohydrolase to obtain citrulline; in the presence of ADP (adenosine diphosphate) and Mg2+, the citrulline is subjected to coupling catalysis through ornithine carbamoyl transferase and carbamyl phosphokinase to finally obtain ornithine, carbon dioxide, ammonia, and ATP, and the released ATP is detectable by reagents. The enzymological detection method has the main advantages that reaction is sensitive, quick and highly accurate, the price is low, the ADMA or citrulline with the least concentration of 0.1 micromol/L can be detected, the detected concentration is approximate to the concentration of the ADMA in normal physiological serum and is far lower than the concentration of citrulline in the normal physiological serum, and a new available method for clinical ADMA detection is provided.