Green tea goat milk with function of reducing blood pressure and preparation method of green tea goat milk
A technology for lowering blood pressure and green tea, which is applied in the field of green tea goat milk and its preparation, and achieves the effects of low production cost, wide application prospects, and mild reaction conditions
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Embodiment 1
[0031] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:
[0032] First: Weigh a certain amount of commercially available goat milk powder, add distilled water at a ratio of 0.5:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 15 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.
[0033] Second: take 100mL skimmed goat milk, add 1mol / L HCl dropwise at 45°C, stir with a magnetic stirrer with a stirring intensity of 1200rpm until pH=4, and obtain goat milk containing casein and whey protein.
[0034]Third: prepare a compound enzyme solution (trypsin and proteinase K, 1:1, v / v) according to a certain ratio, add 5% (v / v), carry out hydrolysis reaction at 45°C, and drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 6.5, stop after 70min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultraviolet spectr...
Embodiment 2
[0039] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:
[0040] First: Weigh a certain amount of commercially available goat milk powder, add distilled water at a ratio of 1:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 30 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.
[0041] Second: take 100mL skimmed goat milk, add 1mol / L HCl dropwise at 50°C, stir with a magnetic stirrer with a stirring intensity of 1200rpm until pH=5.5, and obtain goat milk containing casein and whey protein.
[0042] Third: prepare a compound enzyme solution (trypsin and proteinase K, 2:1, v / v) according to a certain ratio, add 8% (v / v), carry out hydrolysis reaction at 50°C, and drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 7, stop after 90min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultraviolet spectro...
Embodiment 3
[0047] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:
[0048] First: Weigh a certain amount of commercially available goat milk powder, add distilled water according to the ratio of 2:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 40 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.
[0049] Second: Take 100 mL of skimmed goat milk, add 1 mol / L HCl dropwise at 80° C., and stir with a magnetic stirrer with a stirring intensity of 1200 rpm until pH=7. Get goat milk with casein and whey protein.
[0050] Third: prepare a compound enzyme solution (trypsin and proteinase K, 3:1, v / v) according to a certain ratio, add 10% (v / v), carry out hydrolysis reaction at 80 ° C, drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 8.5, stop after 120min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultravi...
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