Green tea goat milk with function of reducing blood pressure and preparation method of green tea goat milk

Abstract

The invention provides green tea goat milk with a function of reducing blood pressure and a preparation method of the green tea goat milk. Goat milk beverage which is rich in ACE inhibitory peptides is prepared by compound enzymolysis, is low in production cost, not limited by resource and small in environmental pollution, and can be mass-produced, and products are safe. On the basis, ultra-fine green tea powder is prepared by enzymic hydrolysis of cell walls and a low-temperature ultra-fine pulverizing technology, specific surface area is increased, and a large number of effective constituents are released. The ultra-fine green tea powder is added in the goat milk in proportion, and the obtained product not only has various nutrient substances and tea fragrance in green tea, but also has treatment effect of reducing high blood pressure, and has wide application prospect in the food industry. The tea powder is prepared by low-temperature ultra-fine pulverization, the original color, flavor and taste quality of tea leaves can be maintained effectively, after the tea leaves is pulverized into ultra-fine powder, the specific surface area of the tea powder is increased obviously, the effective constituents are released easily, human absorption can be improved, and therefore, nutrition absorptivity of human to the tea leaves is improved. Ultra-fine pulverization is a pure physical process, and pure natural property of processed products is maintained.

Description

technical field

[0001] The invention relates to a functional beverage and a preparation method thereof, in particular to a green tea goat's milk with the function of lowering blood pressure and a preparation method thereof. Background technique

[0002] Hypertension seriously endangers human health, causing various cardiovascular and cerebrovascular diseases and kidney diseases, and easily causing stroke and coronary heart disease. The number of hypertensive patients in my country has reached 330 million, and it is becoming younger. The prevalence of hypertension among men aged 25 to 34 has reached 20.4%. Therefore, the prevention and treatment of hypertension is imminent. Angiotensin-converting enzyme (ACE) can degrade bradycarnosine and make it inactive, thereby affecting capillary relaxation and causing blood pressure to increase. The study found that ACE inhibitory peptide, a small molecular polypeptide formed after hydrolyzing food protein, can significantly reduce hi...

Examples

Embodiment 1

[0031] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:

[0032] First: Weigh a certain amount of commercially available goat milk powder, add distilled water at a ratio of 0.5:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 15 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.

[0033] Second: take 100mL skimmed goat milk, add 1mol / L HCl dropwise at 45°C, stir with a magnetic stirrer with a stirring intensity of 1200rpm until pH=4, and obtain goat milk containing casein and whey protein.

[0034]Third: prepare a compound enzyme solution (trypsin and proteinase K, 1:1, v / v) according to a certain ratio, add 5% (v / v), carry out hydrolysis reaction at 45°C, and drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 6.5, stop after 70min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultraviolet spectr...

Embodiment 2

[0039] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:

[0040] First: Weigh a certain amount of commercially available goat milk powder, add distilled water at a ratio of 1:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 30 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.

[0041] Second: take 100mL skimmed goat milk, add 1mol / L HCl dropwise at 50°C, stir with a magnetic stirrer with a stirring intensity of 1200rpm until pH=5.5, and obtain goat milk containing casein and whey protein.

[0042] Third: prepare a compound enzyme solution (trypsin and proteinase K, 2:1, v / v) according to a certain ratio, add 8% (v / v), carry out hydrolysis reaction at 50°C, and drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 7, stop after 90min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultraviolet spectro...

Embodiment 3

[0047] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:

[0048] First: Weigh a certain amount of commercially available goat milk powder, add distilled water according to the ratio of 2:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 40 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.

[0049] Second: Take 100 mL of skimmed goat milk, add 1 mol / L HCl dropwise at 80° C., and stir with a magnetic stirrer with a stirring intensity of 1200 rpm until pH=7. Get goat milk with casein and whey protein.

[0050] Third: prepare a compound enzyme solution (trypsin and proteinase K, 3:1, v / v) according to a certain ratio, add 10% (v / v), carry out hydrolysis reaction at 80 ° C, drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 8.5, stop after 120min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultravi...

technical field

[0001] The invention relates to a functional beverage and a preparation method thereof, in particular to a green tea goat's milk with the function of lowering blood pressure and a preparation method thereof. Background technique

[0002] Hypertension seriously endangers human health, causing various cardiovascular and cerebrovascular diseases and kidney diseases, and easily causing stroke and coronary heart disease. The number of hypertensive patients in my country has reached 330 million, and it is becoming younger. The prevalence of hypertension among men aged 25 to 34 has reached 20.4%. Therefore, the prevention and treatment of hypertension is imminent. Angiotensin-converting enzyme (ACE) can degrade bradycarnosine and make it inactive, thereby affecting capillary relaxation and causing blood pressure to increase. The study found that ACE inhibitory peptide, a small molecular polypeptide formed after hydrolyzing food protein, can significantly reduce hi...

Examples

Embodiment 1

[0031] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:

[0032] First: Weigh a certain amount of commercially available goat milk powder, add distilled water at a ratio of 0.5:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 15 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.

[0033] Second: take 100mL skimmed goat milk, add 1mol / L HCl dropwise at 45°C, stir with a magnetic stirrer with a stirring intensity of 1200rpm until pH=4, and obtain goat milk containing casein and whey protein.

[0034]Third: prepare a compound enzyme solution (trypsin and proteinase K, 1:1, v / v) according to a certain ratio, add 5% (v / v), carry out hydrolysis reaction at 45°C, and drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 6.5, stop after 70min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultraviolet spectr...

Embodiment 2

[0039] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:

[0040] First: Weigh a certain amount of commercially available goat milk powder, add distilled water at a ratio of 1:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 30 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.

[0041] Second: take 100mL skimmed goat milk, add 1mol / L HCl dropwise at 50°C, stir with a magnetic stirrer with a stirring intensity of 1200rpm until pH=5.5, and obtain goat milk containing casein and whey protein.

[0042] Third: prepare a compound enzyme solution (trypsin and proteinase K, 2:1, v / v) according to a certain ratio, add 8% (v / v), carry out hydrolysis reaction at 50°C, and drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 7, stop after 90min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultraviolet spectro...

Embodiment 3

[0047] A method for preparing green tea goat milk rich in ACE inhibitory peptides, comprising the steps of:

[0048] First: Weigh a certain amount of commercially available goat milk powder, add distilled water according to the ratio of 2:9 (w / v) to make reconstituted goat milk, centrifuge at 6000 rpm for 40 minutes at high speed to obtain skim goat milk, and store it at 4°C for later use.

[0049] Second: Take 100 mL of skimmed goat milk, add 1 mol / L HCl dropwise at 80° C., and stir with a magnetic stirrer with a stirring intensity of 1200 rpm until pH=7. Get goat milk with casein and whey protein.

[0050] Third: prepare a compound enzyme solution (trypsin and proteinase K, 3:1, v / v) according to a certain ratio, add 10% (v / v), carry out hydrolysis reaction at 80 ° C, drop in the reaction solution Add 0.1mol / L NaOH to adjust to pH = 8.5, stop after 120min. That is, goat milk rich in ACE inhibitory peptides is obtained, and the ACE inhibitory rate can be measured by ultravi...

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