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An identification method and species technology, applied in biochemical equipment and methods, microbe determination/inspection, chemical instruments and methods, etc., can solve problems such as difficult identification of animal species
Pending Publication Date: 2021-06-08
KOBE UNIV +1
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Although this identification method can be used as an indicator substance for identifying natural leather, it can detect pyrolysis products derived from Hyp equivalent to dipeptides that are not affected by decomposition inhibition caused by pollution, but these pyrolysis products will not be caused by animals. Significant differences occur between different species, making it difficult to identify animal species
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Embodiment 1
[0067] In this example, an example of identification of natural leather of 6 kinds of animals including cattle, horses, pigs, sheep, goats, and deer will be described.
[0068]First, the tissue of the skin of an animal species of natural leather and the collagen of the main fiber will be described. Animal skin consists of the epidermis, dermis and underlying subcutaneous connective tissue. The epidermis is composed of 4 layers, including the cuticle and the granular layer, and the layers that are not used by leather are removed. In addition, the elastin fibers in the papillary layer and subcutaneous tissue are almost completely removed during mechanical manipulation or drug handling during manufacturing. In addition, keratin, which makes up hair, is useless when making leather except for fur, and is decomposed and removed by lime treatment. Therefore, the fibers that make up leather are mostly collagen fibers. The dermis is divided into the papillary layer (silver layer) an...
Embodiment 2
[0111] In this example, an example of identification of animal species of fur such as foxes, raccoons, and minks will be described.
[0112] About the process flow of the identification method of the animal species of the fur of fox, raccoon, mink etc., also same as embodiment 1, follow figure 1 process.
[0113] First, keratin is extracted from the fur of fox, raccoon, mink, etc. (step S101). The extracted keratin is decomposed with keratinase (step S102). As the keratin-decomposing enzyme, trypsin can be used.
[0114]Next, in the same manner as in Example 1, the amino acid sequences of the peptides are analyzed (step S103), the detected peptides that are identical to all identified animal species are removed (step S104), and the peptides detected from the animal species are selected. specific peptides (step S105). Then, use the selected peptides to identify the animal species of the sample (step S106).
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Abstract
The present invention provides an objective method and device for identifying animal species by analyzing peptides to identify animal species. The method includes an analysis step of hydrolyzing animal fibrinome (collagen or the like) extracted from a sample to be identified into peptide fragments with a proteolytic enzyme (protease) and then analyzing the amino acid sequence of the peptide fragments, and a step of removing the same detected peptide fragments as all animal species to be identified from all the analyzed peptide fragments, and a selection step for selecting a peptide fragment detected to have specificity from at least one animal species; and an identification step for identifying the animal species of the sample using the selected peptide fragment. Among the selected peptide fragments, first, the animal species of the sample is identified on the basis of the specific peptide fragment detected only in one specific animal species, and then the animal species can be identified using the specific peptide fragments detected from at least two or more animal species for the sample that has not been identified.
Description
[0001] This application is a divisional application with an application date of March 30, 2015, an application number of 201580078920.8, and an invention title of "Method for Identification of Animal Species". technical field [0002] The present invention relates to a technology related to an objective identification method of animal species, especially a method for identifying leather species. Background technique [0003] According to the Household Goods Quality Indication Law, there are regulations on the quality indication of miscellaneous industrial goods for leather bags, clothes, gloves, and furniture. Among them, for clothing that uses leather or synthetic leather as all or part of the product, identification of the six types of animals (cattle, pigs, sheep, goats, horses, and deer) that are indicated is required. [0004] Recently, in order to express the quality correctly, more and more products are required to have accurate animal identification in the distributi...
Claims
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