Method for preparing D-pantothenic alcohol by using microbial enzyme method

A microbial enzyme and microbial technology, applied in the field of microbial enzymatic preparation of D-panthenol, can solve the problems of many operation steps, low recovery rate of resolving agent, environmental pollution, etc.

Inactive Publication Date: 2002-09-04
HANGZHOU XINFU TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of pantolactone by chemical method is resolved by resolution agents such as chiral amine, quinine compound, dimethyl strychnine and chloramphenicol. The biggest problem is that there are many operation steps, the product is troublesome to refine, and there are It may contain impurities such as chemicals and solvents that are harmful to human and animal health
Also have the resolving agent recovery rate to be low, cause a large amount of resolving agents, such as chloramphicillin, enter waste water, make chloramphicillin account for 70%~80% of the waste water total amount in the waste water, cause the waste water treatment capacity to be big, easily cause environmental pollution
[0005] Although the production of D-pantolactone by Japan's Daiichi Pharmaceutical Co., Ltd. adopts the microbial enzyme production method, there is no report on its synthesis of D-panthenol

Method used

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  • Method for preparing D-pantothenic alcohol by using microbial enzyme method
  • Method for preparing D-pantothenic alcohol by using microbial enzyme method

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Slant culture: the medium is 100ml of potato juice (20%), 2g of glucose, 2g of agar, pH6.5, sterilized at 121°C for 20 minutes, cooled and inoculated after sterilization, and the strain is Fusarium moniliforme (Fusarium moniliforme) SW- 902 CGMCC No.0536, cultured at 25°C for 5-7 days, used as slant activated seeds.

[0015] Enzyme production culture: the medium is 3% glycerol, 0.5% peptone, 0.5% yeast extract, 0.5% corn steep liquor, pH 6.0, the liquid volume is 500ml, 100ml of the triangular bottle, sterilized at 121°C for 20 minutes, and sterilized Afterwards, cool and inoculate the slant seeds, cultivate at 28° C. for 1 to 5 days, filter or centrifuge the fermented liquid, and obtain wet thallus (D-pantolactone hydrolase) as an enzyme source.

[0016] DL-pantolactone prepared by chemical synthesis, with 50mmol CaCl 2 The aqueous solution is prepared as a substrate with a concentration of 30%; the amount of enzyme added is 0.3U / g pantolactone, placed on a shaking ta...

Embodiment 2

[0018] In the reactor, add 2,000Kg of anhydrous methanol and 750Kg of β-aminopropanol, start stirring, put in 1,300Kg of D-pantolactone obtained by the method in Example 1, keep at 40-50°C, react for 24hrs, and depressurize Concentrate until no methanol is distilled off, discharge, and obtain 2,051Kg of colorless transparent viscous liquid D-panthenol, the mol yield to the input D-pantolactone is 99.93%, and the measured specific rotation is [α] D 20 =+30.1°.

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Abstract

The present invention relates to a method for producing D-panalcohol by using D-pantoic acid lactone prepared by microbial enzymic hydrolysis anid beta-aminopropanol and making them produce reaction.This invention utilizes the microbial strain fusarium monoliforme SW-902 (i,e, CGMCC No.0536) which can high-produce D-pantoic acid lactone hydrolase and screened and preserved by the inventor to make fermentation and enzyme production, and then to make hydrolysis of DL-pantoic acid lactone, after lactonization of D-pantoic acid, so as to obtain pure product D-pantoic acid lactone, and the D-pantoic acid lactone and beta-aminopropanal are reacted to obtain the invented D-panalcohol.

Description

technical field [0001] The invention relates to a method for preparing D-panthenol by microbial enzymatic method, in particular to using Fusarium moniliforme (Fusarium moniliforme) SW-902 CGMCC No.0536 to ferment and produce enzyme and carry out enzymatic hydrolysis and splitting to produce D-pantothenic acid Esters, and then react with β-aminopropanol to produce D-panthenol. Background technique [0002] D-pantothenic acid, also known as pantothenic acid, vitamin B 5 or B 3 , is an important drug, food additive and feed additive. Humans and animals have physiological needs for D-pantothenic acid, and D-pantothenic acid is extremely unstable, and its commercial form is D-pantothenate calcium, but D-pantothenate calcium is solid, and its application range is limited, while D-pantothenol, and It is called provitamin B5, which is the equivalent of calcium D-pantothenate in liquid form, which can make up for the deficiency in the application field of calcium D-pantothenate. D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02
Inventor 孙志浩过鑫富汪军
Owner HANGZHOU XINFU TECH CO LTD
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