Method for preparing brain protein hydrolysate for injection and preparation thereof

A technology of protein hydrolyzate and hydrolyzate, which is applied in the field of preparation of cerebroprotein hydrolyzate for injection, and can solve problems such as existing problems in the preparation process

Inactive Publication Date: 2010-12-15
刘燎原 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It also shows that there are still problems in the preparation proc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] (1) Add 20kg of fresh pig brain tissue that has passed the quarantine into 2 times purified water to homogenize,

[0081] (2) Incubate the homogenate at 80°C for 20 minutes, cool to 40°C, add hydrochloric acid to adjust the pH to 3.0, stir and add 600g pepsin, stir, keep at 30-50°C for 4 hours, collect the supernatant, precipitate and centrifuge, and combine the clear liquid;

[0082] (3) The supernatant was concentrated under reduced pressure to 1 / 5 of the weight of the pig's brain. After adjusting the pH value, it was inactivated at 110°C for 6 hours to obtain 4kg of inactivated clear liquid. Add water to the weight of the pig's brain and use Ca(OH) 2 The liquid is adjusted to pH 7.0, bag filtering, activated carbon adsorption, decarbonization, and a clear liquid is obtained.

[0083] (4) Add 400g of pancreatin to the inactivated clear liquid, keep it at 40°C for 3 hours, boil for 30 minutes, cool, adjust the pH to 3.0, stand still, filter the precipitate to obtain the hydro...

Embodiment 2

[0091] (1) Add 40 kg of fresh pig brain tissue qualified for quarantine to 2 times purified water to homogenize,

[0092] (2) Incubate the homogenate at 80°C for 20 minutes, cool to 40°C, add hydrochloric acid to adjust the pH to 3.0, stir and add 1200g pepsin, stir, keep at 42±2°C for 4 hours, collect the supernatant, precipitate and centrifuge, and combine the clear liquid;

[0093] (3) The supernatant was concentrated under reduced pressure to 1 / 5 of the weight of the pig brain. After adjusting the pH value, it was inactivated at 110°C for 6 hours to obtain 8kg of the inactivated clear liquid. Add water to the weight of the pig brain. Use Ca(OH) 2 The liquid is adjusted to pH 7.0, bag filtering, activated carbon adsorption, decarbonization, and a clear liquid is obtained.

[0094] (4) Add 800g of pancreatin to the inactivated clear liquid, keep it at 40±2°C for 3 hours, boil for 30 minutes, cool, adjust the pH to 3.0, stand still, filter the precipitate to obtain the hydrolysate; ...

Embodiment 3

[0102] (1) Add 20kg of fresh pig brain tissue that has passed the quarantine into 2 times purified water to homogenize,

[0103] (2) Incubate the homogenate at 80°C for 20 minutes, cool to 40°C, add hydrochloric acid to adjust the pH to 3.0, stir and add 600g pepsin, stir, keep at 30-50°C for 4 hours, collect the supernatant, precipitate and centrifuge, and combine the clear liquid;

[0104] (3) The supernatant was concentrated under reduced pressure to 1 / 5 of the weight of the pig's brain. After adjusting the pH value, it was inactivated at 110°C for 6 hours to obtain 4kg of inactivated clear liquid. Add water to the weight of the pig's brain and use Ca(OH) 2 The liquid is adjusted to pH 7.0, bag filtering, activated carbon adsorption, decarbonization, and a clear liquid is obtained.

[0105] (4) Add 400g of pancreatin to the inactivated clear liquid, keep it at 40°C for 3 hours, boil for 30 minutes, cool, adjust the pH to 3.0, stand still, filter the precipitate to obtain the hydro...

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Abstract

The invention relates to a method for preparing brain protein hydrolysate for injection and a preparation thereof. The brain protein hydrolysate is prepared from pig brain tissues passing quarantine through a specific controlled enzyme hydrolysis technique, and active ingredients of the prepared brain protein hydrolysate comprise polypeptide with a molecular weight of less than 10,000 Daltons and 16 free amino acids which are hydrolyzed amino acids; each milligram of total nitrogen of the obtained brain protein hydrolysate contains 4.68 to 7.02 milligrams of amino acids; the preparation method comprises the processes of homogenization, primary enzymolysis, inactivation, secondary enzymolysis, cation exchange column adsorption, desorption, blending, ultra-filtration, preparation and the like; when the obtained brain protein hydrolysate is used for preparing the preparation, an amino acid does not need adding; and pharmacological experiment results show that the prepared preparation has good treatment effect and good safety.

Description

technical field [0001] The invention relates to the technical field of biochemical drugs, and more specifically relates to a preparation method and preparation of cerebroprotein hydrolyzate for injection. Background technique [0002] Cerebrolysin hydrolyzate for injection was first sold by the Austrian EBEWE pharmaceutical company under the trade name of Cerebrolysin. Cerebroprotein hydrolyzate is a biochemical drug obtained from healthy pig brain through enzymatic hydrolysis and purification. Its main components are a mixture of various low-molecular-weight peptides and various amino acids. It has a remarkable effect on treating brain functional diseases. [0003] The development of cerebroprotein hydrolyzate started in the 1990s in China. By the end of 2009, 28 patent applications related to the preparation and process of cerebroprotein hydrolyzate had been published. However, almost all preparation processes require external addition of corresponding amino acids in the...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K9/08A61K9/19A61P25/00
Inventor 刘燎原李宏生
Owner 刘燎原
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