Method of extracting diosgenin in Japanese dioscorea by enzymolysis-supercritical carbon dioxide fluid

A diosgenin and supercritical technology is applied in the field of extraction of effective components of natural plants, which can solve the problems of low extraction rate, serious pollution, large consumption of organic solvents and the like, and achieve the effect of simplifying the operation process.

Inactive Publication Date: 2006-06-14
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time to extract, consumes a lot of organic solvents, causes serious pollution, and has a low extraction rate.

Method used

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  • Method of extracting diosgenin in Japanese dioscorea by enzymolysis-supercritical carbon dioxide fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Using cellulase to hydrolyze the supercritical CO of Pangshanlong decoction pieces 2 Fluid extraction diosgenin

[0018] Add 100U cellulase to each gram of P. pangasius decoction pieces, and enzymatically hydrolyze them in a constant temperature water bath at 40°C for 24 hours; hydrolyze the digested panzantines in a 2.0 mol / L sulfuric acid solution for 4.0 h, and then use anhydrous sodium carbonate And drying for standby; crush the dried powder to 40 mesh and add it to the supercritical fluid extraction tank. The process flow is as follows figure 1 Open CO as shown 2 In the steel cylinder, the gas enters the condenser for liquefaction after passing through the purifier I, and then pressurized by the plunger pump and then enters the purifier II for further liquefaction, and enters the bottom of the extractor through the pipeline. Set the pressure of the separator I to 6MPa and the temperature to 60℃ ; The pressure of the secondary separator II is 6MPa, the temper...

Embodiment 2

[0020] Example 2: Using α-amylase to hydrolyze the supercritical CO of Panshan long slices 2 Fluid extraction diosgenin

[0021] Add 100U α-amylase to each gram of raw material of Chinese herbal medicine, and enzymatically hydrolyze it in a constant temperature water bath at 40°C for 24 hours;

[0022] The following process is the same as that of Example 1, and the extraction rate is 2.66%, which is 0.43% higher than that of the dicetaceae slices without enzymatic hydrolysis treatment (the original extraction rate is 2.23%).

Embodiment 3

[0023] Example 3: Supercritical CO with absolute ethanol as carrier agent 2 Fluid extraction diosgenin

[0024] Anhydrous ethanol was used as the carrier, and the others were the same as in Example 1.

[0025] Supercritical CO of the present invention 2 The fluid extraction equipment is selected from Jiangsu Nantong Huaan Supercritical Extraction Co., Ltd., cellulase is produced by He Shibi Biotechnology Co., Ltd., and α-amylase is produced by Beijing Shuangxuan Microbial Culture Medium Products Factory.

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Abstract

The present invention relates to a method for extracting diosgenin from Japanese yam by means of enzymic hydrolysis and supercritical CO2 fluid extraction process. It is characterized by that firstly, it uses cellulose or alpha-diastase to hydrolyze Japanese yam raw material, then using CO2 fluid to make supercritical extraction for extracting diosgenin from Japanese yam.

Description

Technical field [0001] The present invention relates to an enzyme hydrolysis and supercritical CO 2 The fluid extraction technology combined with the method of extracting diosgenin from Dioscorea nigra belongs to the technical field of extraction methods of natural plant active ingredients. Background technique [0002] Diosgenin is a derivative of isospirostene, the molecular formula is C 27 H 42 O 3 With a relative molecular weight of 414.64, it is an important raw material for the synthesis of steroidal contraceptives in the pharmaceutical industry. It is also a raw material for gestational dienolone acetate, an intermediate of steroid hormone drugs. Cortisone, prednisone and norethinol can be synthesized from it. Various steroid hormone drugs, such as ketones, dexamethasone, and dexamethasone, are one of the natural compounds that have attracted much attention. [0003] The traditional extraction and separation technology of diosgenin is that Ma Jingzhong uses acid hydrolysis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
CPCY02P20/54
Inventor 魏福祥王焕
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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